Chromosoma

, Volume 126, Issue 2, pp 213–222 | Cite as

Transcription-replication conflicts at chromosomal fragile sites—consequences in M phase and beyond

Mini-Review

Abstract

Collision between the molecular machineries responsible for transcription and replication is an important source of genome instability. Certain transcribed regions known as chromosomal fragile sites are particularly prone to recombine and mutate in a manner that correlates with specific transcription and replication patterns. At the same time, these chromosomal fragile sites engage in aberrant DNA structures in mitosis. Here, we discuss the mechanistic details of transcription–replication conflicts including putative scenarios for R-loop-induced replication inhibition to understand how transcription–replication conflicts transition from S phase into various aberrant DNA structures in mitosis.

Keywords

Chromosomal fragile sites Transcription Replication Template switching Mitosis Anaphase bridges 

Notes

Acknowledgments

This work was supported by The Danish Agency for Science, Technology and Innovation and the Villum Foundation. We thank Valeria Naim, Xin Shao, Vasileios Voutsinos, and Jakob Nilsson for comments on the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Human and animal rights and informed consent

This article does not contain any studies with human participant or animals performed by any of the authors.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.Department of BiologyUniversity of CopenhagenCopenhagen NDenmark

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