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Assembly and nuclear export of pre-ribosomal particles in budding yeast

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Abstract

The ribosome is responsible for the final step of decoding genetic information into proteins. Therefore, correct assembly of ribosomes is a fundamental task for all living cells. In eukaryotes, the construction of the ribosome which begins in the nucleolus requires coordinated efforts of >350 specialized factors that associate with pre-ribosomal particles at distinct stages to perform specific assembly steps. On their way through the nucleus, diverse energy-consuming enzymes are thought to release assembly factors from maturing pre-ribosomal particles after accomplishing their task(s). Subsequently, recruitment of export factors prepares pre-ribosomal particles for transport through nuclear pore complexes. Pre-ribosomes are exported into the cytoplasm in a functionally inactive state, where they undergo final maturation before initiating translation. Accumulating evidence indicates a tight coupling between nuclear export, cytoplasmic maturation, and final proofreading of the ribosome. In this review, we summarize our current understanding of nuclear export of pre-ribosomal subunits and cytoplasmic maturation steps that render pre-ribosomal subunits translation-competent.

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Acknowledgments

We thank M. Altvater and U. Rothenbusch for comments and suggestions on the manuscript. J. J. Petkowski is supported by an ETH Zürich Postdoctoral Fellowship and the Marie Curie Actions for People COFUND program. V. G. Panse is supported by grants from the Swiss National Science Foundation, the ETH Zürich, and a Starting Grant Award (EURIBIO260676) from the European Research Council.

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  1. Institute of Biochemistry (IBC), ETH Zürich, Otto-Stern-Weg 3, 8093, Zürich, Switzerland

    Stefan Gerhardy, Anna Maria Menet, Cohue Peña, Janusz Jurand Petkowski & Vikram Govind Panse

  2. BSM Program, Life Science Zürich Graduate School, Winterthurerstrasse 190, 8057, Zürich, Switzerland

    Stefan Gerhardy

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  1. Stefan Gerhardy
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  2. Anna Maria Menet
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Correspondence to Vikram Govind Panse.

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Stefan Gerhardy, Anna Maria Menet, Cohue Peña, and Janusz Jurand Petkowski contributed equally to this work.

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Gerhardy, S., Menet, A.M., Peña, C. et al. Assembly and nuclear export of pre-ribosomal particles in budding yeast. Chromosoma 123, 327–344 (2014). https://doi.org/10.1007/s00412-014-0463-z

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