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Radiation and Environmental Biophysics

, Volume 57, Issue 3, pp 215–222 | Cite as

Alterations in histone acetylation following exposure to 60Co γ-rays and their relationship with chromosome damage in human lymphoblastoid cells

  • Xue-Lei Tian
  • Xue Lu
  • Jiang-Bin Feng
  • Tian-Jing Cai
  • Shuang Li
  • Mei Tian
  • Qing-Jie Liu
Original Article
  • 62 Downloads

Abstract

Chromosome damage is related to DNA damage and erroneous repair. It can cause cell dysfunction and ultimately induce carcinogenesis. Histone acetylation is crucial for regulating chromatin structure and DNA damage repair. Ionizing radiation (IR) can alter histone acetylation. However, variations in histone acetylation in response to IR exposure and the relationship between histone acetylation and IR-induced chromosome damage remains unclear. Hence, this study investigated the variation in the total acetylation levels of H3 and H4 in human lymphocytes exposed to 0–2 Gy 60Co γ-rays. Suberoylanilide hydroxamic acid (SAHA), a histone deacetylase (HDAC) inhibitor, was added to modify the histone acetylation state of irradiated cells. Then, the total acetylation level, enzyme activity, dicentric plus centric rings (dic + r) frequencies, and micronucleus (MN) frequencies of the treated cells were analyzed. Results indicated that the acetylation levels of H3 and H4 significantly decreased at 1 and 24 h, respectively, after radiation exposure. The acetylation levels of H3 and H4 in irradiated groups treated with SAHA were significantly higher than those in irradiated groups that were not treated with SAHA. SAHA treatment inhibited HDAC activity in cells exposed to 0–1 Gy 60Co γ-rays. SAHA treatment significantly decreased dic + r/cell and MN/cell in cells exposed to 0.5 or 1.0 Gy 60Co γ-rays relative to that in cells that did not receive SAHA treatment. In conclusion, histone acetylation is significantly affected by IR and is involved in chromosome damage induced by 60Co γ-radiation.

Keywords

Histone acetylation Chromosome damage Histone deacetylase inhibitor Suberoylanilide hydroxamic acid Ionizing radiation 

Notes

Acknowledgements

All authors wish to thank Drs. De-Qing Chen and Ling Gao for their important suggestions. This study was funded by National Natural Science Foundation of China (No. 81573081 to Q.-J. L.) and Youth Science Research Foundation of NIRP, China CDC (No. 2017-002 to X.-L. T.).

Funding

This study was funded by National Natural Science Foundation of China (No. 81573081 to Q.-J. L.) and Youth Science Research Foundation of NIRP, China CDC (No. 2017-002 to X.-L. T.).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

411_2018_742_MOESM1_ESM.docx (19 kb)
Supplementary material 1 (DOCX 19 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Xue-Lei Tian
    • 1
  • Xue Lu
    • 1
  • Jiang-Bin Feng
    • 1
  • Tian-Jing Cai
    • 1
  • Shuang Li
    • 1
  • Mei Tian
    • 1
  • Qing-Jie Liu
    • 1
  1. 1.China CDC Key Laboratory of Radiation Protection and Nuclear Emergency, National Institute for Radiological ProtectionChinese Center for Disease Control and PreventionBeijingPeople’s Republic of China

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