Radiation and Environmental Biophysics

, Volume 57, Issue 2, pp 153–162 | Cite as

Evaporation process in histological tissue sections for neutron autoradiography

  • Natalia M. Espector
  • Agustina Portu
  • Gustavo A. Santa Cruz
  • Gisela Saint Martin
Original Article


The analysis of the distribution and density of nuclear tracks forming an autoradiography in a nuclear track detector (NTD) allows the determination of 10B atoms concentration and location in tissue samples from Boron Neutron Capture Therapy (BNCT) protocols. This knowledge is of great importance for BNCT dosimetry and treatment planning. Tissue sections studied with this technique are obtained by cryosectioning frozen tissue specimens. After the slicing procedure, the tissue section is put on the NTD and the sample starts drying. The thickness varies from its original value allowing more particles to reach the detector and, as the mass of the sample decreases, the boron concentration in the sample increases. So in order to determine the concentration present in the hydrated tissue, the application of corrective coefficients is required. Evaporation mechanisms as well as various factors that could affect the process of mass variation are outlined in this work. Mass evolution for tissue samples coming from BDIX rats was registered with a semimicro analytical scale and measurements were analyzed with software developed to that end. Ambient conditions were simultaneously recorded, obtaining reproducible evaporation curves. Mathematical models found in the literature were applied for the first time to this type of samples and the best fit of the experimental data was determined. The correlation coefficients and the variability of the parameters were evaluated, pointing to Page’s model as the one that best represented the evaporation curves. These studies will contribute to a more precise assessment of boron concentration in tissue samples by the Neutron Autoradiography technique.


Evaporation dynamics Cryosectioning Neutron autoradiography BNCT 



The authors want to thank to Dr. A. Schwint and co-workers for providing biological samples. This work was partially supported by a Grant from Alberto J. Roemmers Foundation.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Facultad de IngenieríaUniversidad FavaloroBuenos AiresArgentina
  2. 2.Comisión de Investigaciones Científicas de la Pcia. de Buenos Aires (CIC)Buenos AiresArgentina
  3. 3.Departamento de RadiobiologíaComisión Nacional de Energía AtómicaSan MartinArgentina
  4. 4.Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)Buenos AiresArgentina
  5. 5.Instituto de Tecnología Jorge SábatoUNSAMBuenos AiresArgentina

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