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Radiation and Environmental Biophysics

, Volume 57, Issue 2, pp 143–152 | Cite as

In vitro studies of DNA damage and repair mechanisms induced by BNCT in a poorly differentiated thyroid carcinoma cell line

  • C. Rodriguez
  • M. Carpano
  • P. Curotto
  • S. Thorp
  • M. Casal
  • G. Juvenal
  • M. Pisarev
  • M. A. Dagrosa
Original Article
  • 180 Downloads

Abstract

Boron neutron capture therapy (BNCT) for aggressive tumors is based on nuclear reaction [10B (n, α) 7Li]. Previously, we demonstrated that BNCT could be applied for the treatment of undifferentiated thyroid carcinoma. The aim of the present study was to describe the DNA damage pattern and the repair pathways that are activated by BNCT in thyroid cells. We analyzed γH2AX foci and the expression of Ku70, Rad51 and Rad54, main effector enzymes of non-homologous end joining (NHEJ) and homologous recombination repair (HRR) pathways, respectively, in thyroid follicular carcinoma cells. The studied groups were: (1) C [no irradiation], (2) gamma [60Co source], (3) N [neutron beam alone], (4) BNCT [neutron beam plus 10 µg 10B/ml of boronphenylalanine (10BPA)]. The total absorbed dose was always 3 Gy. The results showed that the number of nuclear γH2AX foci was higher in the gamma group than in the N and BNCT groups (30 min–24 h) (p < 0.001). However, the focus size was significantly larger in BNCT compared to other groups (p < 0.01). The analysis of repair enzymes showed a significant increase in Rad51 and Rad54 mRNA at 4 and 6 h, respectively; in both N and BNCT groups and the expression of Ku70 did not show significant differences between groups. These findings are consistent with an activation of HRR mechanism in thyroid cells. A melanoma cell line showed different DNA damage pattern and activation of both repair pathways. These results will allow us to evaluate different blocking points, to potentiate the damage induced by BNCT.

Keywords

Damage Repair Pathways BNCT Thyroid cancer 

Notes

Acknowledgements

A part of these studies was supported by grants from the Scientific and Technical Research National Council (CONICET) and Secretary of Science and Technology (SEPCYT).

Compliance with ethical standards

Conflict of interest

We declare that there is no conflict of interest in this paper.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • C. Rodriguez
    • 1
  • M. Carpano
    • 1
  • P. Curotto
    • 3
  • S. Thorp
    • 2
  • M. Casal
    • 4
  • G. Juvenal
    • 1
    • 5
  • M. Pisarev
    • 1
    • 5
  • M. A. Dagrosa
    • 1
    • 5
  1. 1.Radiobiology Department (CAC, CNEA)Buenos AiresArgentina
  2. 2.Instrumentation and Control Department (CAE, CNEA)Buenos AiresArgentina
  3. 3.RA-3-Investigation and Production Reactors (CAE, CNEA)Buenos AiresArgentina
  4. 4.Oncology Institute “Ángel H. Roffo”-University of Buenos AiresCiudad Autónoma de Buenos AiresArgentina
  5. 5.Scientific and Technical Research National Council (CONICET)Ciudad Autónoma de Buenos AiresArgentina

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