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Developing a mouse model of acute bacterial rhinosinusitis

  • Rhinology
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Abstract

The objective of this study is to establish a mouse model of acute bacterial rhinosinusitis. 179 healthy male BALB/c mice were divided into four groups in this randomized and controlled study. Sponge slivers impregnated with methicillin-resistant Staphylococcus aureus (MRSA COL) suspension were inserted into the right nasal cavities for group A; sponge slivers impregnated with sterile saline were inserted into the right nasal cavities for group B; group C mice were inoculated with MRSA COL suspension in right nasal cavities; group D was control group without any treatment. Mice were killed on days 1, 4, 7 and 14, respectively. Nasal lavage fluid was prepared for microbiological culture. Histological examinations of nasal specimens were performed to observe the severity of inflammatory reaction. Acute bacterial rhinosinusitis was induced in all group A mice. Less severe inflammation was seen in partial group B mice compared with that in group A mice (P ≤ 0.05). No inflammatory reaction was found in group C and D mice. In conclusion, a mouse model of acute bacterial rhinosinusitis has been developed successfully using an easier, less invasive and potentially more reversible technique than those used in previous studies.

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Acknowledgments

This study was supported by the National Nature Science Foundation of China (NSFC) 30872849.

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Authors and Affiliations

  1. Department of Otolaryngology, Shengjing Hospital of China Medical University, Sanhao Street 36, Shenyang, Liaoning, China

    Mingzhu Jin, Zhaowei Gu, Zhigang Bian, Jing Yang, Zhiwei Cao & Xiaofeng Yu

  2. Department of Radiotherapy, The Forth Affiliated Hospital of China Medical University, Chongshan North Road 4, Shenyang, Liaoning, China

    Genyan Guo

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  1. Mingzhu Jin
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  2. Zhaowei Gu
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  3. Zhigang Bian
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  4. Jing Yang
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  5. Zhiwei Cao
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  6. Xiaofeng Yu
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  7. Genyan Guo
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Correspondence to Zhiwei Cao.

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Jin, M., Gu, Z., Bian, Z. et al. Developing a mouse model of acute bacterial rhinosinusitis. Eur Arch Otorhinolaryngol 268, 857–861 (2011). https://doi.org/10.1007/s00405-011-1483-4

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  • DOI: https://doi.org/10.1007/s00405-011-1483-4

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