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The impact of the sepsis on female urogenital system: the role of pregabalin



The aim of the study was to investigate the oxidative damage and inflammatory effects of sepsis on the urogenital system in the Lipopolysaccharide (LPS)-induced sepsis model and ameliorating role of Pregabalin (PGB).


Twenty-four female Wistar Albino rats (12 months old) were divided into 3 groups as follows: Sepsis group (Group S) (5 mg/kg LPS, i.p, single dose); Sepsis+ PGB group (Group SP) (5 mg/kg LPS, i.p, single dose and 30 mg/kg PGB); Control group (Group C) (0.1 ml/oral and i.p. saline, single dose), 6 h after LPS administration, the animals were killed. Subsequently, analyses of urogenital tissue oxidant/antioxidant status, histopathological and immunohistochemical analyses were performed.


Total oxidative status (TOS) and oxidative stress index (OSI) values in the urogenital tissues were increased in Group S (Total anti-oxidative status (TAS) decreased) compared to the Control group (p < 0.05). PGB improved these values (p < 0.05). The immunohistochemical markers [Caspase-3, granulocyte colony-stimulating factor (G-CSF), interleukin-6 (IL-6), Serum Amyloid A (SAA) and inducible nitric oxide synthase (iNOS)] were significantly increased in Group S except for bladder (p < 0.001). Statistically significant immunohistochemical positiveness was found only for IL-6 in urinary bladder, though all the others values were negative. With the administration of PGB (Group SP), the expressions of these immunoreactions were markedly decreased (p < 0.001).


These findings demonstrated that sepsis caused oxidative stress and inflammation in the urogenital tissues. We have revealed that PGB ameliorated tissue damage caused by sepsis.

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The authors thank the participants of this study.


This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.

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GI contributed in the literature search, project development, manuscript writing/editing, data collection and interpretation, experimental rat study, rat killing and revised the article; SM contributed in biochemical analysis, statistical analysis, rat killing; OO contributed in histopathological and immunohistochemical analyses, management data analysis, and statistical analysis.

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Correspondence to Ilker Gunyeli.

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Gunyeli, I., Saygin, M. & Ozmen, O. The impact of the sepsis on female urogenital system: the role of pregabalin. Arch Gynecol Obstet 300, 1067–1082 (2019).

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  • Urogenital system
  • Lps-induced rat sepsis
  • Pregabalin