Archives of Gynecology and Obstetrics

, Volume 297, Issue 5, pp 1175–1179 | Cite as

Expression of semaphorin class 3 is higher in the proliferative phase on the human endometrium

  • Gustavo Dias Ferreira
  • Edison Capp
  • Julia Jauckus
  • Thomas Strowitzki
  • Ariane Germeyer
General Gynecology



The semaphorins are related to angiogenesis and cell proliferation depending on the tissue. The purpose of this study was to assess gene expression of class 3 semaphorin (SEMA3A-F) and protein expression of semaphorin 3A (SEMA3A) within human endometrium throughout the menstrual cycle.


Gene expression of SEMA3A-F was analyzed by real-time PCR (qRT-PCR) and protein expression of SEMA3A was analyzed by ELISA in endometrial biopsies in the proliferative and secretory phase of the menstrual cycle.


Gene expression of SEMA3A, SEMA3C, SEMA3D, and SEMA3E was statistically significant decreased in secretory compared to proliferative phase endometrium (p < 0.05). Accordingly, SEMA3A protein expression in the secretory phase was lower than protein expression in proliferative phase endometrium (p ≤ 0.05).


SEMA3A, 3C, 3D, and 3E are possibly related to cell proliferation in the endometrium, being more expressed in the proliferative phase of the cycle. This finding may stimulate studies of class 3 semaphorins as a possible target for treatment of endometrial pathologies.


Endometrium Semaphorin Menstrual cycle SEMA3 



Gustavo Dias Ferreira was scholarship recipient from CAPES—Process Number 18471-12-9.

Author contributions

GDF project development, data management, data analysis, and manuscript writing. EC and AG project development, data analysis, and manuscript editing. JJ project development and data management. TS project development, data collection, and manuscript editing.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. This project was approved by the Ethics Committee of the Medical Faculty of the Heidelberg University (Number 239-05).


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of PhysiologyFederal University of Rio Grande do SulPorto AlegreBrazil
  2. 2.Department of Physiology and PharmacologyFederal University of PelotasPelotasBrazil
  3. 3.Department of Gynecological Endocrinology and Reproductive MedicineHeidelberg UniversityHeidelbergGermany

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