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Interest of Human Papillomavirus DNA quantification and genotyping in paired cervical and urine samples to detect cervical lesions

  • General Gynecology
  • Published:
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Abstract

Background

Cervical cancer is caused by persistent infection with high-risk human papillomavirus (HR-HPV). Conventional human papillomavirus (HPV) testing requires cervical sampling. However, vaginal and urine self-sampling methods are more acceptable for patients and result in increased participation when they are available in screening programs. In this context, we have developed a non-invasive screening method via the detection of HPV DNA in urine samples.

Purpose

To compare HPV viral loads and genotypes in paired cervical and urine samples, and to assess correlation between virological and cytological results in women seeking gynecological consultation.

Methods

Paired urine and cervical specimens were collected and analyzed from 230 of 245 women participating in the previously described prospective PapU study. HPV DNA detection and quantification were performed using a real-time PCR method with short fragment PCR primers. Genotyping was carried out using the INNO-LiPA HPV genotyping assay.

Results

The prevalence of HPV in the 230 paired urine and cervical smear samples was 42 and 49 %, respectively. Overall agreement for HPV positivity and negativity between the paired samples was 90 % (κ = 0.80). High HPV viral load in both cervical and urine samples was associated with cytological abnormalities. HPV-positive women were mostly infected with HR-HPV types. The agreement between high- and low-risk HPV (LR-HPV) detection in both samples was 97 % (κ = 0.95 for HR-HPV and κ = 0.97 for LR-HPV).

Conclusions

High concordance rates for HPV-DNA quantification and high/low-risk HPV genotyping in paired urine/cervical samples suggest that urinary HPV DNA testing could be useful for cervical lesion screening.

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Acknowledgments

We express our thanks to D. Jolivet (Angers) and C. Dolou (Brest) for their helpful assistance in management, A. Pivert, B Mosset for their technical help, and K. L. Erwin for proofreading the English manuscript. The PapU study was initiated by the Angers University hospital with grants from the French Ligue Contre le Cancer, Maine-et-Loire committee (Professor Larra).

Conflict of interest

All authors declared having no conflicts of interest.

Author information

Authors and Affiliations

  1. Laboratoire de Virologie, UPRES EA 3859, Centre Hospitalier Universitaire Angers, 4 rue Larrey, 49000, Angers, France

    A. Ducancelle, A. Pivert, P. Veillon, H. Le Guillou-Guillemette & F. Lunel

  2. Laboratoire de Virologie-LUBEM, Centre Hospitalier Régional Universitaire Brest, Bd Tanguy Prigent, 29609, Brest Cedex, France

    M. C. Legrand & C. Payan

  3. Laboratoire de Cytologie, Centre Hospitalier Universitaire Angers, 4 rue Larrey, 49000, Angers, France

    M. A. De Brux

  4. Laboratoire de Virologie, Centre Hospitalier Universitaire La Milletrie, 2, Rue de La Miletrie BP 577, 86021, Poitiers Cedex, France

    A. Beby-Defaux & G. Agius

  5. Laboratoire de Virologie, Centre Hospitalier Universitaire Dupuytren, 2 Avenue Martin Luther King, 87042, Limoges Cedex, France

    S. Hantz & S. Alain

  6. Service de Gynécologie-Obstétrique, Centre Hospitalier Universitaire Angers, 4 rue Larrey, 49000, Angers, France

    L. Catala & P. Descamps

  7. Service de Gynécologie-Obstétrique, Centre Hospitalier Régional Universitaire Brest, Bd Tanguy Prigent, 29609, Brest Cedex, France

    E. Postec

  8. Service de Gynécologie-Obstétrique, Centre Hospitalier Universitaire Dupuytren, 2 Avenue Martin Luther King, 87042, Limoges Cedex, France

    H. Caly

  9. Laboratoire de Cytologie, Centre Hospitalier Régional Universitaire Brest, Bd Tanguy Prigent, 29609, Brest Cedex, France

    F. Charles-Pétillon

  10. Service D’anatomo-pathologie, Centre Hospitalier Universitaire Dupuytren, 2 Avenue Martin Luther King, 87042, Limoges Cedex, France

    F. Labrousse

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  1. A. Ducancelle
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  2. M. C. Legrand
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  3. A. Pivert
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  17. F. Lunel
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  18. C. Payan
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Corresponding author

Correspondence to A. Ducancelle.

Additional information

F. Lunel and C. Payan contributed equally to this work.

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Ducancelle, A., Legrand, M.C., Pivert, A. et al. Interest of Human Papillomavirus DNA quantification and genotyping in paired cervical and urine samples to detect cervical lesions. Arch Gynecol Obstet 290, 299–308 (2014). https://doi.org/10.1007/s00404-014-3191-y

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  • DOI: https://doi.org/10.1007/s00404-014-3191-y

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