The effect of lyophilized platelet rich-plasma on skin aging: a non-randomized, controlled, pilot trial


Platelet-rich plasma (PRP) showed positive results in the improvement of skin aging. Lyophilized PRP can be interesting in clinical practice due to the facility to obtain many samples in a single blood collection and can be used in multiple injections. To evaluate the effect of lyophilized PRP in the treatment of skin aging, through a Phase II pilot study. Nineteen women (54 years ± 7 years) with Glogau photoaging II and III types were select for this non-randomized, split-face controlled study. They received monthly intradermal injections of lyophilized PRP and saline solution (as control) into the facial skin, during a period of 2 months. The evaluation was performed by imaging method, histological techniques, and multiphoton microscopy. Although lyophilized PRP presented 10 times the platelet baseline value (P < .0001) and growth factors in adequate levels, only saline solution showed an increase of dermis thickness (p = .0009). Collagen pre and post-application remained the same for both types of treatments. The use of lyophilized PRP by mesotherapy showed no improvement on skin aging.

Trial registration approval

RBR-3n9wxw, UTN U1111-1226-6093-retrospectively registered.

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Availability of data and material

Data are available on request from the corresponding author.


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The authors would like to thank the access to equipment and assistance provided by the National Institute of Science and Technology on Photonics Applied to Cell Biology (INFABIC) at UNICAMP; INFABIC is co-funded by Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP) (2014/50938-8) and Conselho Nacional de Desenvolvimento Cientifico e Tecnológico (CNPq) (465699/2014-6). We are also thankful to Professor PA Boer for the support of histological analysis and her knowledge; and R Foglio for correcting our English in the manuscript.


This study was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) – Finance code 2016/14172–6.

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Study concept and design: LQ-S, RBB-C and JMA-B; Acquisition of data and analysis: LQ-S, RBB-C, G-VD and RM-T; Interpretation of data: All authors; Statistical analysis: LQ-S; Critical revision of the manuscript: All authors; Drafting of the manuscript: LQ-S, RBB-C, SAL-M, SC-H and JMA-B; Study supervision: JMA-B; All authors read and approved the final manuscript.

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Correspondence to Letícia Queiroz da Silva.

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Reviewed and approved by Western IRB; approval #30826314.0.0000.5404.

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da Silva, L.Q., Cancela, R.B.B., de Lima Montalvão, S.A. et al. The effect of lyophilized platelet rich-plasma on skin aging: a non-randomized, controlled, pilot trial. Arch Dermatol Res (2021).

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  • Dermatology
  • Clinical research
  • Old-aging
  • Platelet-rich plasma
  • Lyophilization
  • Rejuvenation