The effect of lyophilized platelet rich-plasma on skin aging: a non-randomized, controlled, pilot trial

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Abstract

Platelet-rich plasma (PRP) showed positive results in the improvement of skin aging. Lyophilized PRP can be interesting in clinical practice due to the facility to obtain many samples in a single blood collection and can be used in multiple injections. To evaluate the effect of lyophilized PRP in the treatment of skin aging, through a Phase II pilot study. Nineteen women (54 years ± 7 years) with Glogau photoaging II and III types were select for this non-randomized, split-face controlled study. They received monthly intradermal injections of lyophilized PRP and saline solution (as control) into the facial skin, during a period of 2 months. The evaluation was performed by imaging method, histological techniques, and multiphoton microscopy. Although lyophilized PRP presented 10 times the platelet baseline value (P < .0001) and growth factors in adequate levels, only saline solution showed an increase of dermis thickness (p = .0009). Collagen pre and post-application remained the same for both types of treatments. The use of lyophilized PRP by mesotherapy showed no improvement on skin aging.

Trial registration approval

RBR-3n9wxw, UTN U1111-1226-6093-retrospectively registered.

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Data are available on request from the corresponding author.

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Acknowledgements

The authors would like to thank the access to equipment and assistance provided by the National Institute of Science and Technology on Photonics Applied to Cell Biology (INFABIC) at UNICAMP; INFABIC is co-funded by Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP) (2014/50938-8) and Conselho Nacional de Desenvolvimento Cientifico e Tecnológico (CNPq) (465699/2014-6). We are also thankful to Professor PA Boer for the support of histological analysis and her knowledge; and R Foglio for correcting our English in the manuscript.

Funding

This study was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) – Finance code 2016/14172–6.

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Author notes

  1. Letícia Queiroz da Silva and Rebeca Brito Bonani Cancela authors contributed equally to this publication.

Affiliations

  1. Hemostasis Laboratory - Hemocentro, University of Campinas, 480, Carlos Chagas Street, Campinas, SP, Brazil

    Letícia Queiroz da Silva, Rebeca Brito Bonani Cancela, Silmara Aparecida de Lima Montalvão, Stephany Cares Huber & Joyce Maria Annichino-Bizzacchi

  2. Department of Medicine, Medical Sciences School, University of Campinas, 126, Tessália Vieira de Camargo Street, Campinas, SP, Brazil

    Gislaine Vieira-Damiani

  3. Department of Pathological Anatomy, Hospital das Clínicas, University of Campinas, 251, Vital Brazil Street, Campinas, SP, Brazil

    Renata Marchi Triglia

Authors
  1. Letícia Queiroz da Silva
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  2. Rebeca Brito Bonani Cancela
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Contributions

Study concept and design: LQ-S, RBB-C and JMA-B; Acquisition of data and analysis: LQ-S, RBB-C, G-VD and RM-T; Interpretation of data: All authors; Statistical analysis: LQ-S; Critical revision of the manuscript: All authors; Drafting of the manuscript: LQ-S, RBB-C, SAL-M, SC-H and JMA-B; Study supervision: JMA-B; All authors read and approved the final manuscript.

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Correspondence to Letícia Queiroz da Silva.

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Reviewed and approved by Western IRB; approval #30826314.0.0000.5404.

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da Silva, L.Q., Cancela, R.B.B., de Lima Montalvão, S.A. et al. The effect of lyophilized platelet rich-plasma on skin aging: a non-randomized, controlled, pilot trial. Arch Dermatol Res (2021). https://doi.org/10.1007/s00403-021-02186-2

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Keywords

  • Dermatology
  • Clinical research
  • Old-aging
  • Platelet-rich plasma
  • Lyophilization
  • Rejuvenation