Archives of Dermatological Research

, Volume 310, Issue 3, pp 209–220 | Cite as

Evaluating the accuracy of microRNA27b and microRNA137 as biomarkers of activity and potential malignant transformation in oral lichen planus patients

  • Sana Maher Hasan Aghbari
  • Soheir Mohamed Gaafar
  • Olfat Gamil Shaker
  • Shahira El Ashiry
  • Shaimaa Omar Zayed
Original Paper


Oral lichen planus (OLP) is a chronic inflammatory mucocutaneous disease with a potential malignant transformation, characterized by cytotoxic T cells against basal epithelial cells. MicroRNAs (MiRNAs) are short non-coding RNA that plays critical role in gene expression at post-transcriptional levels. Much evidence showed that miRNAs play an important role in regulating immune response and cancer development. The purpose of the present study was to compare the expression of miRNA 27b and miRNA 137 in tissues and saliva between OLP patients and controls by using RT-qPCR and to evaluate their use as biomarkers of disease activity and potential malignant transformation. Our results showed down expression of miRNA 27b and miRNA 137 in tissue and saliva of OLP patients compared to controls; among OLP subgroups, erosive-type miRNA 137 revealed the lowest level in tissue and saliva. In conclusion, alteration of miRNA 27b and miRNA 137 gene expression signify their use as biomarkers for diseases activity and tendency of malignant transformation, and down expression of miRNA 137 especially in erosive-type favors the use of saliva sample as a noninvasive method for monitoring a potential malignant transformation of OLP.


Oral lichen planus MicroRNA miRNA 27b and miRNA 137 



I would like to express my deepest appreciation to my professors and colleagues in Faculty of Oral and Dental Medicine and in Faculty of Medicine, Cairo University-Egypt.


The study was funded by personal resources.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article was approved by the Faculty of Oral and Dental Medicine Research Ethics committee, Cairo University in September 2013.

Informed consent

Following an explanation of the study as well as information about the sampling procedures, each subject signed a written informed consent form approved by the Faculty Research Ethics committee.


  1. 1.
    Aghbari SM, Abushouk AI, Attia A, Elmaraezy. A, Menshawy. A, Ahmed MS, Elsaadany BA, Ahmed EM (2017) Malignant transformation of oral lichen planus and oral lichenoid lesions: a meta-analysis of 20095 patient data. Oral Oncol 68:92–102. CrossRefPubMedGoogle Scholar
  2. 2.
    Akhtar N, Rasheed Z, Ramamurthy S, Anbazhagan AN, Voss FR, Haqqi TM (2010) MicroRNA-27b regulates the expression of matrix metalloproteinase 13 in human osteoarthritis chondrocytes. Arthritis Rheum 62(5):1361–1371. CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Arão TC, Guimarães ALS, de Paula AMB, Gomes CC, Gomez RS (2012) Increased miRNA-146a and miRNA-155 expressions in oral lichen planus. Arch Dermatol Res 304(5):371–375. CrossRefPubMedGoogle Scholar
  4. 4.
    Bartel DP, Lee R, Feinbaum R (2004) MicroRNAs: genomics, biogenesis, mechanism and function. Cell 116(2):281–297CrossRefPubMedGoogle Scholar
  5. 5.
    Bombeccari GP, Guzzi G, Tettamanti M, Giannì AB, Baj A, Pallotti F, Spadari F (2011) Oral lichen planus and malignant transformation: a longitudinal cohort study. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 112(3):328–334. CrossRefPubMedGoogle Scholar
  6. 6.
    Buck aMYH, Perot J, Chisholm M, Kumar DS, Cognat RIE, Marcinowski L, Tuddenham LEE (2010). Post-transcriptional regulation of miR-27 in murine cytomegalovirus infection. RNA 16(2):307–315. CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Dai R, Ahmed S (2011) microRNA, a new paradigm for underestanding immunoregulation, inflamation an autoimmune diseases. Transl Res 157(4):163–179. CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Dang J, Bian Y, Sun JY, Chen F, Dong G, Liu Q, Wang Q et al. (2013) MicroRNA-137 promoter methylation in oral lichen planus and oral squamous cell carcinoma. J Oral Pathol Med 42(4):315–321. CrossRefPubMedGoogle Scholar
  9. 9.
    Danielsson K, Ebrahimi M, Wahlin YB, Nylander K, Boldrup L (2012) Increased levels of COX-2 in oral lichen planus supports an autoimmune cause of the disease. J Eur Acad Dermatol Venereol 26(11):1415–1419. CrossRefPubMedGoogle Scholar
  10. 10.
    Danielsson K, Wahlin YB, Gu X, Boldrup L, Nylander K (2012) Altered expression of miR-21, miR-125b, and miR-203 indicates a role for these microRNAs in oral lichen planus. J Oral Pathol Med 41(1):90–95. CrossRefPubMedGoogle Scholar
  11. 11.
    Graham JR, Williams CMM, Yang Z (2014) MicroRNA-27b targets gremlin 1 to modulate fibrotic responses in pulmonary cells. J Cell Biochem 115(9):1539–1548. CrossRefPubMedGoogle Scholar
  12. 12.
    Guerra EN, Acevedo AC, Leite AF, Gozal D, Chardin H, De Luca Canto G (2015) Diagnostic capability of salivary biomarkers in the assessment of head and neck cancer: a systematic review and meta-analysis. Oral Oncol 51(9):805–818. CrossRefPubMedGoogle Scholar
  13. 13.
    Hildebrand J, Rütze M, Walz N, Gallinat S, Wenck H, Deppert W, Knott A (2011) A comprehensive analysis of microRNA expression during human keratinocyte differentiation in vitro and in vivo. J Invest Dermatol 131(1):20–29. CrossRefPubMedGoogle Scholar
  14. 14.
    Karbiener M, Fischer C, Nowitsch S, Opriessnig P, Papak C, Ailhaud G, Scheideler M et al. (2009) microRNA miR-27b impairs human adipocyte differentiation and targets PPAR gamma. Biochem Biophys Res Commun 390(2):247–251. CrossRefPubMedGoogle Scholar
  15. 15.
    Kozaki KI, Imoto I, Mogi S, Omura K, Inazawa J (2008) Exploration of tumor-suppressive MicroRNAs silenced by DNA hypermethylation in oral cancer. Cancer Res 68(7):2094–2105. CrossRefPubMedGoogle Scholar
  16. 16.
    Langevin SM, Stone RA, Bunker H, Grandis JR, Sobol RW (2010) MicroRNA-137 promoter methylation in oral rinses from patients with squamous cell carcinoma of the head and neck is associated with gender and body mass index. Carcinogenesis 31(5):864–870. CrossRefPubMedPubMedCentralGoogle Scholar
  17. 17.
    Liu G-X, Sun J-T, Yang M-X, Qi X-M, Shao Q-Q, Xie Q, Sun S-Z et al. (2011) OPN promotes survival of activated T cells by up-regulating CD44 in patients with oral lichen planus. Clin Immunol 138(3):291–298. CrossRefPubMedGoogle Scholar
  18. 18.
    Navazesh M (1993) Methods for collecting saliva. Ann N Y Acad Sci 694:72–77. CrossRefPubMedGoogle Scholar
  19. 19.
    Nylander E, Ebrahimi M, Wahlin YB, Boldrup L, Nylander K (2012) Changes in miRNA expression in sera and correlation to duration of disease in patients with multifocal mucosal lichen planus. J Oral Pathol Med 41(1):86–89. CrossRefPubMedGoogle Scholar
  20. 20.
    Pritchard CC, Cheng HH, Tewari M (2012) MicroRNA profiling: approaches and considerations. Nat Rev Genet 13(5):358–369. CrossRefPubMedPubMedCentralGoogle Scholar
  21. 21.
    Rad M, Hashemipoor MA, Mojtahedi A, Zarei MR, Chamani G, Kakoei S, Izadi N (2009). Correlation between clinical and histopathologic diagnoses of oral lichen planus based on modified WHO diagnostic criteria. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 107(6):796–800. CrossRefPubMedGoogle Scholar
  22. 22.
    Rogler CE, LeVoci L, Ader T, Massimi A, Tchaikovskaya T, Norel R, Rogler LE (2009). MicroRNA-23b cluster microRNAs regulate transforming growth factor-beta/bone morphogenetic protein signaling and liver stem cell differentiation by targeting Smads. Hepatology, 50(2):575–584. CrossRefPubMedGoogle Scholar
  23. 23.
    Schwartz EI, Smilenov LB, Price MA, Osredkar T, Baker RA, Ghosh S, Kruman II (2007). Cell cycle activation in postmitotic neurons is essential for DNA repair. Cell Cycle 6(3):318–329. CrossRefPubMedGoogle Scholar
  24. 24.
    Smith AR, Marquez RT, Tsao W, Pathak S, Roy A, Ping J, Kristi L (2015) Tumor suppressive microRNA-137 negatively Musashi-1 and colorectal cancer progression regulates. Oncotarget 6(14):12558–12573. CrossRefPubMedPubMedCentralGoogle Scholar
  25. 25.
    Tao X-A, Li C-Y, Xia J, Yang X, Chen X-H, Jian Y-T, Cheng B (2009) Differential gene expression profiles of whole lesions from patients with oral lichen planus. J Oral Pathol Med 38(5):427–433. CrossRefPubMedGoogle Scholar
  26. 26.
    Wang J-M, Tao J, Chen D-D, Cai J-J, Irani K, Wang Q, Chen AF (2014) MicroRNA miR-27b rescues bone marrow-derived angiogenic cell function and accelerates wound healing in type 2 diabetes mellitus. Arterioscler Thromb Vasc Biol 34(1):99–109. CrossRefPubMedGoogle Scholar
  27. 27.
    Zhang W, Liu W, Zhou Y, Shen X, Wang Y, Tang G (2012) Altered microRNA expression profile with miR-27b down-regulation correlated with disease activity of oral lichen planus. Oral Dis 18(3):265–270. CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Sana Maher Hasan Aghbari
    • 1
  • Soheir Mohamed Gaafar
    • 2
  • Olfat Gamil Shaker
    • 3
  • Shahira El Ashiry
    • 2
  • Shaimaa Omar Zayed
    • 4
  1. 1.Faculty of DentistryAden UniversityAdenYemen
  2. 2.Faculty of Oral and Dental MedicineCairo UniversityCairoEgypt
  3. 3.Faculty of MedicineCairo UniversityCairoEgypt
  4. 4.Department of Oral PathologyFaculty of Oral and Dental Medicine, Cairo UniversityCairoEgypt

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