Evaluating the accuracy of microRNA27b and microRNA137 as biomarkers of activity and potential malignant transformation in oral lichen planus patients
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Oral lichen planus (OLP) is a chronic inflammatory mucocutaneous disease with a potential malignant transformation, characterized by cytotoxic T cells against basal epithelial cells. MicroRNAs (MiRNAs) are short non-coding RNA that plays critical role in gene expression at post-transcriptional levels. Much evidence showed that miRNAs play an important role in regulating immune response and cancer development. The purpose of the present study was to compare the expression of miRNA 27b and miRNA 137 in tissues and saliva between OLP patients and controls by using RT-qPCR and to evaluate their use as biomarkers of disease activity and potential malignant transformation. Our results showed down expression of miRNA 27b and miRNA 137 in tissue and saliva of OLP patients compared to controls; among OLP subgroups, erosive-type miRNA 137 revealed the lowest level in tissue and saliva. In conclusion, alteration of miRNA 27b and miRNA 137 gene expression signify their use as biomarkers for diseases activity and tendency of malignant transformation, and down expression of miRNA 137 especially in erosive-type favors the use of saliva sample as a noninvasive method for monitoring a potential malignant transformation of OLP.
KeywordsOral lichen planus MicroRNA miRNA 27b and miRNA 137
I would like to express my deepest appreciation to my professors and colleagues in Faculty of Oral and Dental Medicine and in Faculty of Medicine, Cairo University-Egypt.
The study was funded by personal resources.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article was approved by the Faculty of Oral and Dental Medicine Research Ethics committee, Cairo University in September 2013.
Following an explanation of the study as well as information about the sampling procedures, each subject signed a written informed consent form approved by the Faculty Research Ethics committee.
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