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Archives of Dermatological Research

, Volume 310, Issue 3, pp 241–243 | Cite as

Construction of a plasmid vector containing epidermal growth factor receptor and C-Jun shRNA

  • Hong Xiao
  • Ruinian Yang
  • Fang Yang
  • Yanan Zhao
  • Yin Liu
Concise Communication
  • 152 Downloads

Abstract

The objective of this study was to construct a plasmid vector for EGFR-hm-1 and C-Junh-825 small interfering RNA (siRNA). EGFR-hm-1 and C-Jun-hm-825 oligonucleotide fragments were synthesized and short hairpin RNA (shRNA) were amplified by PCR. Plasmids were isolated from E. coli TOP10 bacterium by restriction enzyme digestion using pst1 and BamH1 and oligonucleotide fragments were cloned into the pSilencer plasmid containing the U6 promoter. Recombinant clones were generated by transforming JM109 competent cells with plasmid vectors containing shRNA molecules. 58 base-paired EGFR-hm-1 and 59 base-paired C-Jun-hm-825 oligonucleotide fragments were isolated. The fragments were 100% homologous with human sequences available on GenBank. The recombinant pSilencer1.0 vector containing a 58-bp EGFR-hm-1 and 59-bp C-Jun-hm-825 fragment was constructed. These vectors have the potential to be used as treatment to combat skin photoaging under UV exposure.

Keywords

Plasmid C-Jun Epidermal growth factor receptor ShRNA pSilencer1.0 Restriction digestion 

Notes

Funding

There is no funding source.

Compliance with ethical standards

Conflict of interest

The authors have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Hong Xiao
    • 1
  • Ruinian Yang
    • 2
  • Fang Yang
    • 1
  • Yanan Zhao
    • 1
  • Yin Liu
    • 1
  1. 1.Department of Plastic SurgeryThe Second Affiliated Hospital of Kunming Medical UniversityKunmingChina
  2. 2.Department of Plastic SurgeryYESTAR Aesthetic Plastic HospitalKunmingChina

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