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Hematoxylin-stainability of keratohyalin granules is due to the novel component, fibrinogen γ-chain protein

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Abstract

Hematoxylin-stainability of keratohyalin granules (KHG) using biochemical and immunohistochemical techniques is due to the presence of a fibrinogen γ-chain protein. A protein with a molecular weight of 100 kDa was stained with anti-Ted-H-1 monoclonal antibody and hematoxylin solution (hematoxylin-stainable protein). Since the amino acid sequence of the hematoxylin-stainable protein was to that of fibrinogen γ-chain protein, a peptide was synthesized and an antibody against the peptide was produced. This antibody reacted with the hematoxylin-stainable protein and fibrinogen γ-chain protein on immunoblot analysis and with KHG on immunohistochemical examination. Furthermore, a commercial anti-fibrinogen γ-chain protein antibody (Ab) also reacted with the hematoxylin-stainable protein as well as fibrinogen. In contrast, anti-fibrinogen β-chain protein Ab did not react with the hematoxylin-stainable protein. The fibrinogen γ-chain protein also stained with hematoxylin. These findings suggested that fibrinogen γ-chain protein may be a novel component protein of KHG and may induce the hematoxylin-stainability of KHG.

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Abbreviations

KHG:

Keratohyalin granules

MoAb:

Monoclonal antibody

Ab:

Antibody

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Authors and Affiliations

  1. Institute for Health Sciences, Tokushima Bunri University, Yamashiro, Tokushima, Tokushima, 770-8514, Japan

    Masae Takahashi

  2. Life Science Institute, Kinki University, 377-2 Ohno-Higashi, Osaka-Sayama, Osaka, 589-8511, Japan

    Yoshitaka Horiuchi

  3. Department of Dermatology, Kinki University School of Medicine, 377-2 Ohno-Higashi, Osaka-Sayama, Osaka, 589-8511, Japan

    Tadashi Tezuka

Authors
  1. Masae Takahashi
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  2. Yoshitaka Horiuchi
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Correspondence to Masae Takahashi.

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Takahashi, M., Horiuchi, Y. & Tezuka, T. Hematoxylin-stainability of keratohyalin granules is due to the novel component, fibrinogen γ-chain protein. Arch Dermatol Res 302, 679–684 (2010). https://doi.org/10.1007/s00403-010-1077-9

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  • DOI: https://doi.org/10.1007/s00403-010-1077-9

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