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Properties and histochemical application of a novel antibody against trichohyalin granules

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We obtained an antibody, anti-inner root sheath cells antibody (anti-IRSC Ab), that reacted with the inner root sheath (IRS) cells especially trichohyalin granules (THG). In order to compare the properties of anti-IRSC Ab and AE15, which is a specific monoclonal antibody against THG, histochemical and biochemical examinations were performed. In vivo localization with anti-IRSC Ab and AE15 indicated that both antibodies reacted with THG, but anti-IRSC Ab reacted with THG in the suprabulbar region of the Huxley layer, whereas AE15 reacted with THG in the suprabulbar region and upper bulbar portion of the Huxley layer, as shown by immunohistochemical and immunoelectron microscopic analyses. The results of immunoblot analysis showed that anti-IRSC Ab reacted with a protein spot at 45 kDa, pI 6.5, but AE15 reacted with high molecular weight proteins at pI 5.5. Furthermore, anti-IRSC Ab reacted with specimens of squamous cell carcinoma (SCC) but did not react with those of basal cell carcinoma (BCC). In contrast, AE15 reacted with neither SCC nor BCC. These findings suggest that anti-IRSC Ab and AE 15 recognized different component proteins in THG, and therefore indicated that THG, like as keratohyalin granules, might consist of several proteins. It is the novel finding that the anti-IRSC Ab positive substance in THG in the normal hair and SCC cells.

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Abbreviations

THG:

Trichohyalin granules

SCAMP:

Secretory carrier membrane protein

IRS:

Inner root sheath

ORS:

Outer root sheath

Ab:

Aantibody

SCC:

Squamous cell carcinoma

BCC:

Basal cell carcinoma

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Acknowledgment

We thank Prof. M. Manabe, Department of Dermatology, Akita University School of Medicine, for providing AE15.

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Authors and Affiliations

  1. Institute for Health Sciences, Tokushima Bunri University, Yamashiro, Tokushima, 770-8514, Japan

    Masae Takahashi

  2. Life Science Institute, Kinki University, 377-2 Ohno-Higashi, Osaka-Sayama, Osaka, 589-8511, Japan

    Yoshitaka Horiuchi

  3. Department of Dermatology, Kinki University School of Medicine, 377-2 Ohno-Higashi, Osaka-Sayama, Osaka, 589-8511, Japan

    Masuki Yoshida, Rieko Isogai & Akira Kawada

  4. Nakatsu Skin Clinic, Kitaku, Osaka, 531-0072, Japan

    Tadashi Tezuka

Authors
  1. Masae Takahashi
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  2. Yoshitaka Horiuchi
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  3. Masuki Yoshida
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  4. Rieko Isogai
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  5. Akira Kawada
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  6. Tadashi Tezuka
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Correspondence to Masae Takahashi.

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Takahashi, M., Horiuchi, Y., Yoshida, M. et al. Properties and histochemical application of a novel antibody against trichohyalin granules. Arch Dermatol Res 299, 33–39 (2007). https://doi.org/10.1007/s00403-007-0736-y

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  • DOI: https://doi.org/10.1007/s00403-007-0736-y

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