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Table 1 Commonly used immunomarkers for the characterization of different microglial states in the healthy and diseased murine and human CNS are shown

From: Analyzing microglial phenotypes across neuropathologies: a practical guide

Epitope Description Usage Recommended antibody
CD11b Commonly used for flow cytometry (in combination with CD45) for the isolation of microglia cells/CAMs FC Human: Biolegend, cat. # 301310, 1:20
Mice: eBioscience, cat. # 17-0112-83, 1:200
CD163 Marker for perivascular macrophages. Parenchymal microglia may upregulate CD163 upon activation IHC Novus, cat. # NB110-40686, 1:100
CD206 Marker for perivascular macrophages IHC BioRad, cat. # MCA2235, 1:100
CD45 Commonly used for flow cytometry (in combination with CD11b) for the isolation of microglia cells/CAMs FC Human: Biolegend, cat. # 304008, 1:20
Mice: eBioscience, cat. # 12-0451-83, 1:200
CD68 Marker for lysosomal activity IHC Human: DAKO, cat. # M0876, 1:400
Mice: BioRad, cat. # MCA1957, 1:100
F4/80 Commonly used myeloid marker in mice IHC BioRad, cat. # MCA497GA, 1:100
Iba1 Pan-myeloid marker labelling microglia, perivascular macrophages, meningeal macrophages and infiltrating myeloid cells IHC abcam, cat. # ab178846, 1:1000
Mac-3 Marker for lysosomal activity IHC Becton Dickinson, 553322, 1:400
MHC-II Antigen-presenting cells IHC Human: Dako, cat. # M0775, 1:400
Mice: Abcam, cat. # ab23990, 1:400
P2RY12 Marker for microglia cells; absent in perivascular macrophages or meningeal macrophages. Of note, parenchymal microglia cells may downregulate P2RY12 upon activation. Consequently, a ramified P2RY12-positive cell in the brain parenchyma can be identified as a microglia cell. IHC Anaspec, Cat. # AS-55043A, 1:200
Pu.1 Nuclear pan-myeloid marker IHC, FC Cell signaling technology, cat. # 2258S, 1:200
SLC2A5 Glucose transporter type 5 labelling parenchymal myeloid cells IHC Abcam, Atlas Antibodies, HPA005449, 1:250
TMEM119 Marker for microglia cells; absent in perivascular macrophages or meningeal macrophages. Of note, parenchymal microglia cells may downregulate TMEM119 upon activation. Consequently, a ramified TMEM119-positive cell in the brain parenchyma can be identified as a microglia cell. IHC Human: Abcam, cat. # ab185333, 1:250
Mice: Abcam, cat. # ab209064, 1:500