Acta Neuropathologica

, Volume 131, Issue 1, pp 151–154 | Cite as

Non-prion-type transmission in A53T α-synuclein transgenic mice: a normal component of spinal homogenates from naïve non-transgenic mice induces robust α-synuclein pathology

  • Amanda N. Sacino
  • Jacob I. Ayers
  • Mieu M. T. Brooks
  • Paramita Chakrabarty
  • Vincent J. HudsonIII
  • Jasie K. Howard
  • Todd E. Golde
  • Benoit I. Giasson
  • David R. Borchelt
Correspondence

Supplementary material

401_2015_1505_MOESM1_ESM.docx (42 kb)
Supplementary material 1 (DOCX 42 kb)
401_2015_1505_MOESM2_ESM.tif (5.4 mb)
Supplementary material 2 (TIFF 5573 kb)
401_2015_1505_MOESM3_ESM.tif (79.8 mb)
Supplementary material 3 (TIFF 81760 kb)
401_2015_1505_MOESM4_ESM.tif (10.5 mb)
Supplementary material 4 (TIFF 10753 kb)

References

  1. 1.
    Betemps D, Verchere J, Brot S, Morignat E, Bousset L, Gaillard D, Lakhdar L, Melki R, Baron T (2014) Alpha-synuclein spreading in M83 mice brain revealed by detection of pathological alpha-synuclein by enhanced ELISA. Acta Neuropathol Commun 2:29. doi:10.1186/2051-5960-2-29 PubMedPubMedCentralCrossRefGoogle Scholar
  2. 2.
    Giasson BI, Duda JE, Quinn SM, Zhang B, Trojanowski JQ, Lee VM (2002) Neuronal alpha-synucleinopathy with severe movement disorder in mice expressing A53T human alpha-synuclein. Neuron 34:521–533 (S0896627302006827 [pii]) PubMedCrossRefGoogle Scholar
  3. 3.
    Luk KC, Kehm VM, Zhang B, O’Brien P, Trojanowski JQ, Lee VM (2012) Intracerebral inoculation of pathological alpha-synuclein initiates a rapidly progressive neurodegenerative alpha-synucleinopathy in mice. J Exp Med 209:975–986. doi:10.1084/jem.20112457 PubMedPubMedCentralCrossRefGoogle Scholar
  4. 4.
    Mougenot AL, Nicot S, Bencsik A, Morignat E, Verchere J, Lakhdar L, Legastelois S, Baron T (2012) Prion-like acceleration of a synucleinopathy in a transgenic mouse model. Neurobiol Aging 33:2225–2228. doi:10.1016/j.neurobiolaging.2011.06.022 PubMedCrossRefGoogle Scholar
  5. 5.
    Prusiner SB, Woerman AL, Mordes DA, Watts JC, Rampersaud R, Berry DB, Patel S, Oehler A, Lowe JK, Kravitz SN et al (2015) Evidence for alpha-synuclein prions causing multiple system atrophy in humans with parkinsonism. Proc Natl Acad Sci USA. doi:10.1073/pnas.1514475112
  6. 6.
    Rutherford NJ, Sacino AN, Brooks M, Ceballos-Diaz C, Ladd TB, Howard JK, Golde TE, Giasson BI (2015) Studies of lipopolysaccharide effects on the induction of alpha-synuclein pathology by exogenous fibrils in transgenic mice. Mol Neurodegener 10:32. doi:10.1186/s13024-015-0029-4 PubMedPubMedCentralCrossRefGoogle Scholar
  7. 7.
    Sacino AN, Brooks M, Thomas MA, McKinney AB, Lee S, Regenhardt RW, McGarvey NH, Ayers JI, Notterpek L, Borchelt DR et al (2014) Intramuscular injection of alpha-synuclein induces CNS alpha-synuclein pathology and a rapid-onset motor phenotype in transgenic mice. Proc Natl Acad Sci USA 111:10732–10737. doi:10.1073/pnas.1321785111 PubMedPubMedCentralCrossRefGoogle Scholar
  8. 8.
    Sacino AN, Brooks M, Thomas MA, McKinney AB, McGarvey NH, Rutherford NJ, Ceballos-Diaz C, Robertson J, Golde TE, Giasson BI (2014) Amyloidogenic alpha-synuclein seeds do not invariably induce rapid, widespread pathology in mice. Acta Neuropathol 127:645–665. doi:10.1007/s00401-014-1268-0 PubMedCrossRefGoogle Scholar
  9. 9.
    Watts JC, Giles K, Oehler A, Middleton L, Dexter DT, Gentleman SM, DeArmond SJ, Prusiner SB (2013) Transmission of multiple system atrophy prions to transgenic mice. Proc Natl Acad Sci USA 110:19555–19560. doi:10.1073/pnas.1318268110 PubMedPubMedCentralCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Amanda N. Sacino
    • 1
    • 2
  • Jacob I. Ayers
    • 1
    • 2
    • 3
  • Mieu M. T. Brooks
    • 1
    • 2
  • Paramita Chakrabarty
    • 1
    • 2
    • 3
  • Vincent J. HudsonIII
    • 1
    • 2
  • Jasie K. Howard
    • 1
    • 2
  • Todd E. Golde
    • 1
    • 2
    • 3
  • Benoit I. Giasson
    • 1
    • 2
    • 3
  • David R. Borchelt
    • 1
    • 2
    • 3
  1. 1.Department of NeuroscienceCollege of Medicine University of FloridaGainesvilleUSA
  2. 2.Center for Translational Research in Neurodegenerative DiseaseCollege of Medicine University of FloridaGainesvilleUSA
  3. 3.McKnight Brain InstituteCollege of Medicine University of FloridaGainesvilleUSA

Personalised recommendations