Advertisement

Colloid and Polymer Science

, Volume 296, Issue 5, pp 917–926 | Cite as

Effect of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/gelatin ratios on the characteristics of biomimetic composite nanofibrous scaffolds

  • Mi-Ok Choi
  • Young-Jin Kim
Original Contribution

Abstract

Biomimetic composite nanofibrous scaffolds were fabricated via the growth of calcium phosphate (CaP) crystals on electrospun poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)/gelatin (PHGE) nanofibers with different polymer ratio to mimic the composite nature of bone tissue as well as the nanoscale features of extracellular matrix (ECM). The resulting composite scaffolds exhibited three-dimensionally interconnected microporous structures. The CaP crystals were successfully formed on not only the external surface but also the interior of the scaffolds. The amount of CaP crystals fabricated and the surface roughness of the scaffolds increased with increasing the PHBV content because of the formation of bead-typed CaP aggregates. Higher amount of CaP crystals significantly accelerated the deposit rate of bone-like apatite on the surface of composite membrane. The results of cytocompatibility tests demonstrated that PHGE41 scaffold, composed of PHBV/gelatin (4:1), promoted more rapid MC3T3-E1 proliferation and differentiation compared with other scaffolds. These results suggest that the PHGE composite scaffolds are ideal biomaterials for bone tissue engineering.

Keywords

Biomimetic Calcium phosphate Composite scaffold Electrospinning Mineralization 

Notes

Funding information

This work was partially supported by the Industrial Technology Innovation Program of the Korea Institute for Advancement of Technology (KIAT) grant funded by the Ministry of Trade, Industry & Energy (MOTIE) (R0005364) and by the Technology Innovation Program (10053595, Development of functionalized hydrogel scaffold based on medical grade biomaterials with 30% or less of molecular weight reduction) funded by the MOTIE, Republic of Korea.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. 1.
    Amrita AA, Sharma P, Katti DS (2015) Pullulan-based composite scaffolds for bone tissue engineering: improved osteoconductivity by pore wall mineralization. Carbohydr Polym 123:180–189.  https://doi.org/10.1016/j.carbpol.2015.01.038 CrossRefGoogle Scholar
  2. 2.
    Zhang H, Fu QW, Sun TW, Chen F, Qi C, Wu J, Cai ZY, Qian QR, Zhu YJ (2015) Amorphous calcium phosphate, hydroxyapatite and poly(D,L-lactic acid) composite nanofibers: electrospinning preparation, mineralization and in vivo bone defect repair. Colloid Surf B: Biointerfaces 136:27–36.  https://doi.org/10.1016/j.colsurfb.2015.08.015 CrossRefGoogle Scholar
  3. 3.
    Liu X, Smith LA, Hu J, Ma PX (2009) Biomimetic nanofibrous gelatin/apatite composite scaffolds for bone tissue engineering. Biomaterials 30:2252–2258.  https://doi.org/10.1016/j.biomaterials.2008.12.068 CrossRefGoogle Scholar
  4. 4.
    Whited BM, Whitney JR, Hofmann MC, Xu Y, Rylander MN (2011) Pre-osteoblast infiltration and differentiation in highly porous apatite-coated PLGA electrospun scaffolds. Biomaterials 32:2294–2304.  https://doi.org/10.1016/j.biomaterials.2010.12.003 CrossRefGoogle Scholar
  5. 5.
    Fujiwara K, Okada M, Takeda S, Matsumoto N (2014) A novel strategy for preparing nanoporous biphasic calcium phosphate of controlled composition via a modified nanoparticle-assembly method. Mater. Sci. Eng. C 35:259–266.  https://doi.org/10.1016/j.msec.2013.11.019 CrossRefGoogle Scholar
  6. 6.
    Dhand C, Ong ST, Dwivedi N, Diaz SM, Venugopal JR, Navaneethan B, Fazil MHUT, Liu S, Seitz V, Wintermantel E, Beuerman RW, Ramakrishna S, Verma NK, Lakshminarayanan R (2016) Bio-inspired in situ crosslinking and mineralization of electrospun collagen scaffolds for bone tissue engineering. Biomaterials 104:323–338.  https://doi.org/10.1016/j.biomaterials.2016.07.007 CrossRefGoogle Scholar
  7. 7.
    Gupta D, Venugopal J, Mitra S, Giri Dev VR, Ramakrishna S (2009) Nanostructured biocomposite substrates by electrospinning and electrospraying for the mineralization of osteoblasts. Biomaterials 30:2085–2094.  https://doi.org/10.1016/j.biomaterials.2008.12.079 CrossRefGoogle Scholar
  8. 8.
    Bai J, Dai J, Li G (2015) Electrospun composites of PHBV/pearl powder for bone repairing. Prog Nat Sci 25:327–333.  https://doi.org/10.1016/j.pnsc.2015.07.004 CrossRefGoogle Scholar
  9. 9.
    Goonoo N, Bhaw-Luximon A, Passanha P, Esteves S, Schönherr H, Jhurry D (2017) Biomineralization potential and cellular response of PHB and PHBV blends with natural anionic polysaccharides. Mater. Sci. Eng. C 76:13–24.  https://doi.org/10.1016/j.msec.2017.02.156 CrossRefGoogle Scholar
  10. 10.
    Fayyazbakhsh F, Solati-Hashjin M, Keshtkar A, Shokrgozar MA, Dehghan MM, Larijani B (2017) Novel layered double hydroxides-hydroxyapatite/gelatin bone tissue engineering scaffolds: fabrication, characterization, and in vivo study. Mater. Sci. Eng. C 76:701–714.  https://doi.org/10.1016/j.msec.2017.02.172 CrossRefGoogle Scholar
  11. 11.
    Meng W, Xing ZC, Jung KH, Kim SY, Yuan J, Kang IK, Yoon SC, Shin HI (2008) Synthesis of gelatin-containing PHBV nanofiber mats for biomedical application. J Mater Sci Mater Med 19:2799–2807.  https://doi.org/10.1007/s10856-007-3356-3 CrossRefGoogle Scholar
  12. 12.
    Paşcu EI, Stokes J, McGuinness GB (2013) Electrospun composites of PHBV, silk fibroin and nano-hydroxyapatite for bone tissue engineering. Mater Sci Eng C 33:4905–4916.  https://doi.org/10.1016/j.msec.2013.08.012 CrossRefGoogle Scholar
  13. 13.
    Kokubo T (1991) Bioactive glass ceramics: properties and applications. Biomaterials 12:155–163CrossRefGoogle Scholar
  14. 14.
    Qian J, Ma J, Su J, Yan Y, Li H, Shin JW, Wei J, Zhao L (2016) PHBV-based ternary composite by intermixing of magnesium calcium phosphate nanoparticles and zein: in vitro bioactivity, degradability and cytocompatibility. Eur Polym J 75:291–302.  https://doi.org/10.1016/j.eurpolymj.2015.12.026 CrossRefGoogle Scholar
  15. 15.
    Ramírez-Rodríguez GB, Delgado-López JM, Iafisco M, Montesi M, Sandri M, Sprio S, Tampieri A (2016) Biomimetic mineralization of recombinant collagen type I derived protein to obtain hybrid matrices for bone regeneration. J Struct Biol 196:138–146.  https://doi.org/10.1016/j.jsb.2016.06.025 CrossRefGoogle Scholar
  16. 16.
    Zhang Y, Ai J, Wang D, Hong Z, Li W, Yokogawa Y (2013) Dissolution properties of different composition of biphasic calcium phosphate bimodal porous ceramics following immersion in simulated body fluid solution. Ceram Int 39:6751–6762.  https://doi.org/10.1016/j.ceramint.2013.02.004 CrossRefGoogle Scholar
  17. 17.
    Kumar A, Dhara S, Biswas K, Basu B (2013) In vitro bioactivity and cytocompatibility properties of spark plasma sintered HA-Ti composites. J Biomed Mater Res Part B 101B:223–236.  https://doi.org/10.1002/jbm.b.32829 CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Biomedical EngineeringDaegu Catholic UniversityGyeongsanRepublic of Korea

Personalised recommendations