Effect of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/gelatin ratios on the characteristics of biomimetic composite nanofibrous scaffolds
Biomimetic composite nanofibrous scaffolds were fabricated via the growth of calcium phosphate (CaP) crystals on electrospun poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)/gelatin (PHGE) nanofibers with different polymer ratio to mimic the composite nature of bone tissue as well as the nanoscale features of extracellular matrix (ECM). The resulting composite scaffolds exhibited three-dimensionally interconnected microporous structures. The CaP crystals were successfully formed on not only the external surface but also the interior of the scaffolds. The amount of CaP crystals fabricated and the surface roughness of the scaffolds increased with increasing the PHBV content because of the formation of bead-typed CaP aggregates. Higher amount of CaP crystals significantly accelerated the deposit rate of bone-like apatite on the surface of composite membrane. The results of cytocompatibility tests demonstrated that PHGE41 scaffold, composed of PHBV/gelatin (4:1), promoted more rapid MC3T3-E1 proliferation and differentiation compared with other scaffolds. These results suggest that the PHGE composite scaffolds are ideal biomaterials for bone tissue engineering.
KeywordsBiomimetic Calcium phosphate Composite scaffold Electrospinning Mineralization
This work was partially supported by the Industrial Technology Innovation Program of the Korea Institute for Advancement of Technology (KIAT) grant funded by the Ministry of Trade, Industry & Energy (MOTIE) (R0005364) and by the Technology Innovation Program (10053595, Development of functionalized hydrogel scaffold based on medical grade biomaterials with 30% or less of molecular weight reduction) funded by the MOTIE, Republic of Korea.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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