Co-ingestion of NUTRALYS® pea protein and a high-carbohydrate beverage influences the glycaemic, insulinaemic, glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) responses: preliminary results of a randomised controlled trial

Abstract

Purpose

Plant-based proteins may have the potential to improve glycaemic and gastrointestinal hormone responses to foods and beverages. The aim of this study was to investigate the effect of two doses of pea protein on postprandial glycaemic, insulinaemic, glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) response following a high-carbohydrate beverage intake in healthy individuals.

Methods

In a single-blind, randomised, controlled, repeat measure, crossover design trial, thirty-one participants were randomly assigned to ingest 50 g glucose (Control), 50 g glucose with 25 g pea protein (Test 1) and 50 g glucose with 50 g pea protein (Test 2) on three separate days. Capillary blood samples (blood glucose and plasma insulin measurements) and venous blood samples (GIP and GLP-1 concentrations) were taken before each test and at fixed intervals for 180 min. The data were compared using repeated-measures ANOVA or the Friedman test.

Results

Glucose incremental Area under the Curve (iAUC180) was significantly lower (p < 0.001) after Test 2 compared with Control (− 53%), after Test 1 compared with Control (− 31%) and after Test 2 compared with Test 1 (−32%). Insulin iAUC 180 was significantly higher (p < 0.001) for Test 1 (+ 28%) and Test 2 (+ 40%) compared with Control and for Test 2 (+ 17%) compared with Test 1 (p = 0.003). GIP and GLP-1 release showed no clear difference between Control and Pea protein drinks.

Conclusion

The consumption of pea protein reduced postprandial glycaemia and stimulated insulin release in healthy adults with a dose–response effect, supporting its role in regulating glycaemic and insulinaemic responses.

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References

  1. 1.

    Barclay AW, Petocz P, McMillan-Price J et al (2008) Glycemic index, glycemic load and chronic disease risk – a meta-analysis of observational studies. Am J Clin Nutr 87:627–637

    CAS  Article  Google Scholar 

  2. 2.

    Riccardi G, Rivellese AA, Giacco R (2008) Role of glycemic index and glycemic load in the healthy state, in prediabetes and diabetes. Am J Clin Nutr 87:269S-274S

    CAS  Article  Google Scholar 

  3. 3.

    Sloth B, Krog-Mikkelsen I, Flint A et al (2004) No difference in body weight decrease between a low-glycemic-index and a high-glycemic-index diet but reduced LDL cholesterol after 10-wk ad libitum intake of the low-glycemic-index diet. Am J Clin Nutr 80:337–347

    CAS  Article  Google Scholar 

  4. 4.

    Jenkins DJA, Kendall CWC, McKeown-Eyssen G et al (2008) Effect of a low glycemic index or a high cereal fibre diet on type 2 diabetes A randomized trial. JAMA 300:2742–2753

    CAS  Article  Google Scholar 

  5. 5.

    Burani J, Longo PJ (2006) Low-glycemic index carbohydrates: an effective behavioral change for glycemic control and weight management in patients with type 1 and 2 diabetes. Diabetes Educ 32:78–88

    Article  Google Scholar 

  6. 6.

    Wu CL, Williams C (2006) A low glycemic index meal before exercise improves endurance running capacity in men. Int J Sport Nutr Exercise Metabol 16:510–527

    CAS  Article  Google Scholar 

  7. 7.

    Solomon TPJ, Haus JM, Kelly KR et al (2010) A low glycemic index diet combined with exercise reduced insulin resistance, postprandial hyperinsulinemia and glucose-dependent insulinotropic polypeptide responses in obese prediabetic humans. Am J Clin Nutr 92:1359–1368

    CAS  Article  Google Scholar 

  8. 8.

    Nilsson AC, Ostman EM, Granfeldt Y, Bjorck IME (2008) Effect of cereal test breakfasts differing in glycemic index and content of indigestible carbohydrates on daylong glucose tolerance in healthy subjects. Am J Clin Nutr 87:645–654

    CAS  Article  Google Scholar 

  9. 9.

    Hermansen MF, Eriksen NMB, Mortensen LS, Holm L, Hermansen K (2006) Can the glycemic index (GI) be used as a tool in the prevention and management of type 2 diabetes? Rev Diabet Stud 3:61–71

    Article  Google Scholar 

  10. 10.

    Collier GR, Greenberg GR, Wolever TM, Jenkins DJ (1988) The acute effect of fat on insulin secretion. J Clin Endocrinol Metab 66:323–326

    CAS  Article  Google Scholar 

  11. 11.

    Frid AH, Nilsson M, Holst JJ, Bjorck IME (2005) Effect of whey on blood glucose and insulin responses to composite breakfast and lunch meals in type 2 diabetic subjects. Am J Clin Nutr 82:69–75

    CAS  Article  Google Scholar 

  12. 12.

    Wachters-Hagedoorn RE, Priebe MG, Heimweg JA et al (2006) The rate of intestinal glucose absorption is correlated with plasma glucose-dependent insulinotropic polypeptide concentrations in healthy men. J Nutr 136:1511–1516

    CAS  Article  Google Scholar 

  13. 13.

    Eelderink C, Schepers M, Preston T, Vonk RJ, Oudhuis L, Priebe MG (2012) Slowly and rapidly digestible starchy foods can elicit a similar glycemic response because of differential tissue glucose uptake in healthy men. Am J Clin Nutr 96:1017–1024

    CAS  Article  Google Scholar 

  14. 14.

    Péronnet F, Meynier A, Sauvinet V et al (2015) Plasma glucose kinetics and response of insulin and GIP following a cereal breakfast in female subjects: effect of starch digestibility. Eur J Clin Nutr 69:740–745

    Article  Google Scholar 

  15. 15.

    Keyhani-Nejad F, Irmler M, Isken F et al (2015) Nutritional strategy to prevent fatty liver and insulin resistance independent of obesity by reducing glucose-dependent insulinotropic polypeptide responses in mice. Diabetologia 58:374–383

    CAS  Article  Google Scholar 

  16. 16.

    Drucker D (2006) The biology of incretin hormones. Cell Metab 1:3153–3165

    Google Scholar 

  17. 17.

    Holst J (2007) The physiology of glucagon-like peptide 1. Physiol Rev 87:1409–1439

    CAS  Article  Google Scholar 

  18. 18.

    Dahl WJ, Foster LM, Tyler RT (2012) Review of the health benefits of peas (Pisum sativum L.). Br J Nutr 108:S3–S10

    CAS  Article  Google Scholar 

  19. 19.

    Mollard RC, Luhovyy BL, Smith C, Anderson GH (2014) Acute effects of pea protein and hull fibre alone and combined on blood glucose, appetite, and food intake in healthy young men - a randomized crossover trial. Appl Physiol Nutr Metab 39:1360–1365

    CAS  Article  Google Scholar 

  20. 20.

    Tan S-Y, Siow PC, Peh E, Henry CJ (2018) Influence of rice, pea and oat proteins in attenuating glycemic response of sugar-sweetened beverages. Eur J Nutr 57:2795–2803

    CAS  Article  Google Scholar 

  21. 21.

    Re R, Pombo S, Calame W, Lefranc-Millot C, Guérin-Deremaux L (2016) The satiating effect of NUTRALYS® pea protein leads to reduced energy intake in healthy humans. J Nutr Health Food Sci 4:1–10

    Google Scholar 

  22. 22.

    Smith CE, Mollard RC, Luhovyy BL, Anderson GH (2012) The effect of yellow pea protein and fibre on short-term food intake, subjective appetite and glycaemic response in healthy young men. Br J Nutr 108:S74–S80

    CAS  Article  Google Scholar 

  23. 23.

    Karamanlis A, Chaikomin R, Doran S, Bellon M, Bartholomeusz FD, Wishart JM, Jones KL, Horowitz M, Rayner CK (2007) Effects of protein on glycemic and incretin responses and gastric emptying after oral glucose in healthy subjects. Am J Clin Nutr 86:1364–1368

    CAS  Article  Google Scholar 

  24. 24.

    Manders RJ, Little JP, Forbes SC, Candow DG (2012) Insulinotropic and muscle protein synthetic effects of branched-chain amino acids: potential therapy for type 2 diabetes and sarcopenia. Nutr 4:1664–1678

    CAS  Google Scholar 

  25. 25.

    Rietman A, Schwarz J, Tome D, Kok FJ, Mensink M (2014) High dietary protein intake, reducing or eliciting insulin resistance? Eur J Clin Nutr 68:973–979

    CAS  Article  Google Scholar 

  26. 26.

    Azemati B, Rajaram S, Jaceldo-Siegl K, Sabate J, Shavlik D, Fraser GE, Haddad EH (2017) Animal-protein intake is associated with insulin resistance in adventist health study 2 (AHS-2) calibration substudy participants: a cross-sectional analysis. Curr Dev Nutr 1(4):000299. https://doi.org/10.3945/cdn.116.000299

    Article  Google Scholar 

  27. 27.

    Markova M, Hornemann S, Sucher S, Wegner K, Pivovarova O, Rudovich N, Thomann R, Schneeweiss R, Rohn S, Pfeiffer AFH (2018) Rate of appearance of amino acids after a meal regulates insulin and glucagon secretion in patients with type 2 diabetes: a randomized clinical trial. Am J Clin Nutr 108:279–291

    Article  Google Scholar 

  28. 28.

    Seino Y, Fukushima M, Yabe D (2010) GIP and GLP-1, the two incretin hormones: similarities and differences. J Diabetes Investig 1:8–23

    CAS  Article  Google Scholar 

  29. 29.

    Kahleova H, Tura A, Klementova M, Thieme L, Haluzik M, Pavlovicova R, Hill M, Pelikanova T (2019) A plant-based meal stimulated incretin and insulin secretion more than an energy- and macronutrient-matched standard meal in type 2 diabetes: a randomized crossover study. Nutrients 11:486

    CAS  Article  Google Scholar 

  30. 30.

    Smith K, Davies KAB, Stevenson EJ, West DJ (2020) The clinical application of mealtime whey protein for the treatment of postprandial hyperglycaemia for people with type 2 diabetes: a long whey to go. Front Nutr 7:587843

    Article  Google Scholar 

  31. 31.

    Pfeiffer AFH, Keyhani-Nejad F (2018) High glycemic index metabolic damage – a pivotal role of GIP and GLP-1. Trends Endocrinol Metab 29:289–299

    CAS  Article  Google Scholar 

  32. 32.

    Vosloo MC (2005) Some factors affecting the digestion of glycaemic carbohydrates and the blood glucose response. J Family Ecol Consumer Sci 33:1–9

    Google Scholar 

  33. 33.

    Anderson GH, Liu Y, Smith CE, Liu TT, Nunez MF, Mollard RC, Luhovyy BL (2014) The acute effect of commercially available pulse powders on postprandial glycaemic response in healthy young men. Br J Nutr 112:1966–1973

    CAS  Article  Google Scholar 

  34. 34.

    Beaudry KM, Devries MC (2019) Nutritional strategies to combat type 2 diabetes in aging adults: the importance of protein. Front Nutr 6:138. https://doi.org/10.3389/fnut.2019.00138

    CAS  Article  PubMed  PubMed Central  Google Scholar 

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Acknowledgments

We would like to thank all participants in this study. We would like to thank Nasim Soleymani Majd for her assistance with blood analysis and Jonathan Tammam for reviewing one of the drafts. This study was supported by a grant from Roquette Frères, France.

Funding

This study was supported by funding from Roquette Frères, France.

Author information

Affiliations

Authors

Contributions

HL, PST, EA, LG-D and CL-M contributed to the development of the study protocol. Material preparation and data collection were performed by PST, IA, AS and TM. Data analysis was performed by PST, IA and HL. The first draft of the manuscript was written by HL. PST, LG-D and CL-M revised the subsequent drafts of the manuscript. All authors have read and approved the final manuscript.

Corresponding author

Correspondence to Pariyarath Sangeetha Thondre.

Ethics declarations

Conflict of interest

L Guérin-Deremaux and C Lefranc-Millot are employees of Roquette Frères. Roquette Frères did not play a part in the execution of the study or analysis of the results.

Ethics approval

The study was carried out in accordance with the declaration of World Medical Association Declaration of Helsinki. Ethical approval was obtained from the University Research Ethics Committee (UREC) at Oxford Brookes University (UREC Registration No: 181259).

Informed consent

Participants were given full details of the study protocol and the opportunity to ask questions. All participants gave written informed consent prior to participation.

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Thondre, P.S., Achebe, I., Sampson, A. et al. Co-ingestion of NUTRALYS® pea protein and a high-carbohydrate beverage influences the glycaemic, insulinaemic, glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) responses: preliminary results of a randomised controlled trial. Eur J Nutr (2021). https://doi.org/10.1007/s00394-021-02481-8

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Keywords

  • Pea protein
  • Blood glucose
  • Insulin
  • Glucose-dependent insulinotropic polypeptide
  • Glucagon-like peptide-1