Tuscany Sangiovese grape juice imparts cardioprotection by regulating gene expression of cardioprotective C-type natriuretic peptide



A regular intake of red grape juice has cardioprotective properties, but its role on the modulation of natriuretic peptides (NPs), in particular of C-type NP (CNP), has not yet been proven. The aims were to evaluate: (1) in vivo the effects of long-term intake of Tuscany Sangiovese grape juice (SGJ) on the NPs system in a mouse model of myocardial infarction (MI); (2) in vitro the response to SGJ small RNAs of murine MCEC-1 under physiological and ischemic condition; (3) the activation of CNP/NPR-B/NPR-C in healthy human subjects after 7 days’ SGJ regular intake.


(1) C57BL/6J male and female mice (n = 33) were randomly subdivided into: SHAM (n = 7), MI (n = 15) and MI fed for 4 weeks with a normal chow supplemented with Tuscany SGJ (25% vol/vol, 200 µl/per day) (MI + SGJ, n = 11). Echocardiography and histological analyses were performed. Myocardial NPs transcriptional profile was investigated by Real-Time PCR. (2) MCEC-1 were treated for 24 h with a pool of SGJ small RNAs and cell viability under 24 h exposure to H2O2 was evaluated by MTT assay. (3) Human blood samples were collected from seven subjects before and after the 7 days’ intake of Tuscany SGJ. NPs and miRNA transcriptional profile were investigated by Real-Time PCR in MCEC-1 and human blood.


Our experimental data, obtained in a multimodal pipeline, suggest that the long-term intake of SGJ promotes an adaptive response of the myocardium to the ischemic microenvironment through the modulation of the cardiac CNP/NPR-B/NPR-C system.


Our results open new avenue in the development of functional foods aimed at enhancing cardioprotection of infarcted hearts through action on the myocardial epigenome.

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This study was conducted within the context of the project entitled Cardio.MIR.San.To (Bando Nutraceutica DD 650/2014), supported by the Regione Toscana (Tuscany Region). We are grateful to Fattoria Viticcio (Greve in Chianti, Italy) for technical support.

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SDR, VL, BS, MEP: designed research; BS, MC, MM, CP: conducted research; SDR, VL, BS: analyzed and interpreted data and wrote the manuscript. SDR, VL, MEP had primary responsibility for the final content. All authors read and approved the final manuscript.

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Correspondence to S. Del Ry.

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Svezia, B., Cabiati, M., Matteucci, M. et al. Tuscany Sangiovese grape juice imparts cardioprotection by regulating gene expression of cardioprotective C-type natriuretic peptide. Eur J Nutr 59, 2953–2968 (2020). https://doi.org/10.1007/s00394-019-02134-x

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  • Sangiovese grape juice
  • Functional food
  • Natriuretic peptide system
  • C-type natriuretic peptide
  • Plant miRNAs
  • Myocardial infarction