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Inter-individual variability in the production of flavan-3-ol colonic metabolites: preliminary elucidation of urinary metabotypes

  • Pedro Mena
  • Iziar A. Ludwig
  • Virginia B. Tomatis
  • Animesh Acharjee
  • Luca Calani
  • Alice Rosi
  • Furio Brighenti
  • Sumantra Ray
  • Julian L. Griffin
  • Les J. Bluck
  • Daniele Del Rio
Original Contribution

Abstract

Purpose

There is much information on the bioavailability of (poly)phenolic compounds following acute intake of various foods. However, there are only limited data on the effects of repeated and combined exposure to specific (poly)phenol food sources and the inter-individual variability in their bioavailability. This study evaluated the combined urinary excretion of (poly)phenols from green tea and coffee following daily consumption by healthy subjects in free-living conditions. The inter-individual variability in the production of phenolic metabolites was also investigated.

Methods

Eleven participants consumed both tablets of green tea and green coffee bean extracts daily for 8 weeks and 24-h urine was collected on five different occasions. The urinary profile of phenolic metabolites and a set of multivariate statistical tests were used to investigate the putative existence of characteristic metabotypes in the production of flavan-3-ol microbial metabolites.

Results

(Poly)phenolic compounds in the green tea and green coffee bean extracts were absorbed and excreted after simultaneous consumption, with green tea resulting in more inter-individual variability in urinary excretion of phenolic metabolites. Three metabotypes in the production of flavan-3-ol microbial metabolites were tentatively defined, characterized by the excretion of different amounts of trihydroxyphenyl-γ-valerolactones, dihydroxyphenyl-γ-valerolactones, and hydroxyphenylpropionic acids.

Conclusions

The selective production of microbiota-derived metabolites from flavan-3-ols and the putative existence of characteristic metabotypes in their production represent an important development in the study of the bioavailability of plant bioactives. These observations will contribute to better understand the health effects and individual differences associated with consumption of flavan-3-ols, arguably the main class of flavonoids in the human diet.

Keywords

Polyphenols Green tea catechins Coffee caffeoylquinic acids Colonic microbiota Urinary phenotype Metabotypes 

Notes

Acknowledgements

We thank the volunteers who participated in the study, Polly Page for her key role in study steering and management oversight, and the Volunteer Studies and Clinical Services and Sample Management Teams at MRC EWL for their assistance in the conduction of the study. We acknowledge Prof. Alan Crozier (University of California, Davis, USA) for his help with manuscript revision and data discussion. We are also grateful to Gary Williamson (University of Leeds, UK), Denis Barron (Nestle Research Center, Lausanne, Switzerland), and Takao Yokota (Teikyo University, Japan) for the generous gift of a number of phase II metabolites. Dr. Les Bluck, joint senior author for this work, played a fundamental role in the design of the original study; it is with much sadness that his death prevented him from seeing the research come to fruition.

Author contributions

PM and IL designed and conducted research, analyzed data, performed statistical analysis, and wrote the paper; VT designed and conducted research, analyzed data, and performed statistical analysis; AA performed statistical analysis; LC conducted research; AR, FB, and JLG provided critical review of the manuscript; SR designed and conducted research, and provided critical review; LJB designed research; DDR designed research and had primary responsibility for final content. All authors read and approved the final manuscript.

Funding

This work was partially funded by MRC core funding (Physiological Modelling of Metabolic Risk, MC_UP_A090_1005, and Nutrition, Surveys and Studies, MC_U105960384) and University of Parma core funding (FIL 2014-2017). P.M. was partially funded by a Grant of the Postdoctoral Fellowship Program from Fundación Séneca (Murcia Region, Spain). I.A.L. was supported by a postdoctoral fellowship funded by the Spanish Ministry of Economy and Competitiveness (IJCI-2014-20689).

Compliance with ethical standards

Conflict of interest

Authors declare no conflict of interest.

Supplementary material

394_2018_1683_MOESM1_ESM.docx (2.8 mb)
Supplementary material 1 (DOCX 2909 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Human Nutrition Unit, Department of Food and DrugsUniversity of ParmaParmaItaly
  2. 2.Food Technology DepartmentUniversitat de Lleida-Agrotecnio CenterLleidaSpain
  3. 3.UK Medical Research Council Elsie Widdowson Laboratory (formerly MRC Human Nutrition Research)CambridgeUK
  4. 4.Department of BiochemistryUniversity of CambridgeCambridgeUK
  5. 5.Institute of Cancer and Genomic Sciences, Centre for Computational BiologyUniversity of BirminghamBirminghamUK
  6. 6.Institute of Translational MedicineUniversity Hospitals Birmingham NHS Foundation TrustBirminghamUK

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