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MALDI-Massenspektrometrie am Meniskus

Objektivierung morphologischer Befunde

MALDI mass spectrometry of the meniscus

Objectification of morphological findings

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Zeitschrift für Rheumatologie Aims and scope Submit manuscript

Zusammenfassung

Hintergrund

Die Einordnung von Meniskusläsionen erfordert in erster Linie adäquate klinische Angaben. Zur Vereinheitlichung des histopathologischen Befundberichts ist eine Einteilung in normal, Low-grade- und High-grade-Läsionen praktikabel und sinnvoll. Diese Einordung kann durch weitere Methoden präzisiert werden.

Material und Methoden

Formalinfixierte und in Paraffin eingebettete Meniskusgewebsproben von 68 Patienten wurden mittels matrixunterstützter Laser-Desorption/Ionisierung(MALDI)-Bildgebung analysiert.

Ergebnisse

Eine Klassifikation zur Unterscheidung von „low grade“ vs. „high grade“ bzw. zwischen akuter und nichtakuter Meniskusdegeneration konnte durch differenziell exprimierte Masse-Ladungs-Verhältnisse (m/z) anhand eines statistischen Vergleichs mittels Wilcoxon-/Kruskal-Wallis-Test und eines zuvor definierten durchschnittlich 2-fach erhöhten Intensitätsunterschieds determiniert werden.

Schlussfolgerung

Zur Befunddokumentation wird ein „Meniskusreport“ vorgestellt, der die histologischen, histochemischen und proteomischen Daten einschließt und somit Aussagen zur Degeneration und zur Frage einer akuten oder älteren Rissbildung präzisiert. Massenspektrometrische Methoden tragen zur Objektivierung der histopathologischen Diagnostik der Meniskusläsionen bei. Eine gutachterliche Äußerung ist nur möglich, wenn alle klinischen und morphologischen Daten vorliegen und korreliert werden.

Abstract

Background

The classification of meniscal lesions requires correlation with clinical data. For the standardization of histopathology reports a discrimination between normal, low-grade lesions and high-grade lesions is feasible. This classification can be further specified using other methods.

Material and methods

Formalin-fixed, paraffin-embedded specimens of meniscal tissue from 68 patients were analyzed by matrix-assisted laser desorption ionization (MALDI) imaging.

Results

The classification of meniscal lesions and differentiation between low-grade and high-grade and acute versus non-acute degeneration is possible by determination of the differential expression of mass-to-charge ratios by statistical comparisons using the P-value from combined Wilcoxon and Kruskal-Wallis (PWKW) tests and a predefined average two-fold difference in intensity.

Conclusion

The concept of a “meniscus report” is introduced for documentation of meniscus tissue specimens integrating histological, histochemical and proteomic data, thereby specifying the degree of degeneration and the assessment of acute or non-acute lesions. Mass spectrometry contributes to an objective histopathology report. An advisory opinion should always be based on close correlation of clinical and morphological evaluations.

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Einhaltung ethischer Richtlinien

Interessenkonflikt. J. Petzold, R. Casadonte, M. Otto, M. Kriegsmann, M. Granrath, A. Baltzer, J. Vogel, P. Drees, S. Deininger, M. Becker und J. Kriegsmann geben an, dass kein Interessenkonflikt besteht. Dieser Beitrag beinhaltet keine Studien an Menschen oder Tieren.

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Authors and Affiliations

  1. Zentrum für Molekulare Orthopädie, Gemeinschaftspraxis Königsallee, Düsseldorf, Deutschland

    J. Petzold, M. Granrath & A. Baltzer

  2. Proteopath GbR, Trier, Deutschland

    R. Casadonte, M. Otto &  J. Kriegsmann

  3. MVZ für Histologie, Zytologie und Molekulare Diagnostik Trier, Max-Planck-Str. 5, 54296, Trier, Deutschland

    M. Otto &  J. Kriegsmann

  4. Institut für Pathologie, Ruprecht-Karls-Universität Heidelberg, Heidelberg, Deutschland

    M. Kriegsmann

  5. Orthopaedicum, Trier, Deutschland

    J. Vogel

  6. Zentrum für Orthopädie und Unfallchirurgie, Universitätsklinikum der Johannes-Gutenberg-Universität Mainz, Mainz, Deutschland

    P. Drees

  7. Bruker Daltonik GmbH, Bremen, Deutschland

    S. Deininger & M. Becker

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Petzold, J., Casadonte, R., Otto, M. et al. MALDI-Massenspektrometrie am Meniskus. Z Rheumatol 74, 438–446 (2015). https://doi.org/10.1007/s00393-014-1468-y

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  • DOI: https://doi.org/10.1007/s00393-014-1468-y

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