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Effect of testicular ischemia-reperfusion on recruitment of neutrophils, E-selectin expression and germ cell apoptosis in the contralateral testis in a rat

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Abstract

Recent evidence suggests that neutrophil recruitment may initiate germ cell apoptosis in the ischemic testis. The purpose of the present study was to examine the relationship between germ cell apoptosis and neutrophil recruitment in the contralateral testis following testicular ischemia-reperfusion (IR) injury in a rat. Adult male Sprague–Dawley rats were divided randomly into two experimental groups: Group A: Sham operated animals; Group B: IR rats underwent 90 min of unilateral testicular ischemia following by 96 h of reperfusion. The rats were sacrificed and testes were harvested. Johnsen’s criteria and the number of germinal cell layers were measured to categorize the spermatogenesis. TUNEL assay was used to determine germ cell apoptosis in both the ischemic and contralateral testis. The recruitment of neutrophils was calculated per 100 venules. Expression of E-selectin was determined using immunohistochemical analysis. Statistical analysis was performed using Student's t test, with P less than 0.05 considered statistically significant. Germ cell apoptosis in both the ischemic and the contralateral testis increased significantly after IR. E-selectin expression was significantly greater in ischemic testis from IR rats compared to sham animals. The small increase in E-selectin expression and the concomitant increase in neutrophil recruitment in the contralateral testis of the IR rats (vs. sham animals) were not statistically significant. In conclusion, testicular ischemia causes an increase in germ cell apoptosis in the contralateral testis. Mechanisms other than neutrophil recruitment apparently initiate this process.

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Authors and Affiliations

  1. Department of Pediatric Surgery and Pathology, Bnai Zion Medical Center, Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, 47 Golomb St., P.O.B. 4940, Haifa, 31048, Israel

    Igor Sukhotnik, Katya Voskoboinik, Michael Lurie, Robert Greenblatt, Samuel Eldar & Jorge G. Mogilner

  2. Department of Surgery, Carmel Medical Center, 7 Mihal St., Haifa, 34362, Israel

    Eitan Shiloni

  3. Section of Pediatric Surgery, F3970, Mott Children’s Hospital, University of Michigan, 1500 East Medical Center Drive, Ann Arbor, MI, 48109-0245, USA

    Arnold G. Coran

Authors
  1. Igor Sukhotnik
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  2. Katya Voskoboinik
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  3. Michael Lurie
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  4. Arnold G. Coran
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  5. Robert Greenblatt
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  6. Eitan Shiloni
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  7. Samuel Eldar
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  8. Jorge G. Mogilner
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Corresponding author

Correspondence to Jorge G. Mogilner.

Additional information

Igor Sukhotnik and Katya Voskoboinik contributed equally to the preparation of this manuscript.

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Sukhotnik, I., Voskoboinik, K., Lurie, M. et al. Effect of testicular ischemia-reperfusion on recruitment of neutrophils, E-selectin expression and germ cell apoptosis in the contralateral testis in a rat. Pediatr Surg Int 23, 479–485 (2007). https://doi.org/10.1007/s00383-006-1854-x

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  • DOI: https://doi.org/10.1007/s00383-006-1854-x

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