Association of main folate metabolic pathway gene polymorphisms with neural tube defects in Han population of Northern China
Neural tube defects (NTDs) are one of the most prevalent and the most severe congenital malformations worldwide. Studies have confirmed that folic acid supplementation could effectively reduce NTDs risk, but the genetic mechanism remains unclear. In this study, we explored association of single nucleotide polymorphisms (SNP) within folate metabolic pathway genes with NTDs in Han population of Northern China.
We performed a case-control study to compare genotype and allele distributions of SNPs in 152 patients with NTDs and 169 controls. A total of 16 SNPs within five genes were genotyped by the Sequenom MassARRAY assay.
Our results indicated that three SNPs associated significantly with NTDs (P<0.05). For rs2236225 within MTHFD1, children with allele A or genotype AA had a high NTDs risk (OR=1.500, 95%CI=1.061~2.120; OR=2.862, 95%CI=1.022~8.015, respectively). For rs1801133 within MTHFR, NTDs risk markedly increased in patients with allele T or genotype TT (OR=1.552, 95%CI=1.130~2.131; OR=2.344, 95%CI=1.233~4.457, respectively). For rs1801394 within MTRR, children carrying allele G and genotype GG had a higher NTDs risk (OR=1.533, 95%CI=1.102~2.188; OR=2.355, 95%CI=1.044~5.312, respectively).
Our results suggest that rs2236225 of MTHFD1 gene, rs1801133 of MTHFR gene and rs1801394 of MTRR gene were associated with NTDs in Han population of Northern China.
KeywordsNeural tube defects Polymorphisms Folate metabolism Susceptibility
We are grateful to all the families for their NTDs-affected children enrolled in this study. We also extend our thanks to the staff of Tianjin Children’s Hospital for their cooperation and support in the collection of samples.
This research was supported by the National 973 Program on Population and Health (no. 2013CB945404), the Natural Science Foundation of Tianjin City of China (no. 14JCYBJC25000), the Ministry of Science and Technology of P.R. China, and the Project of Tianjin Health Care Professionals (no. 15KR12), the Key Project of Tianjin Health Care Professionals (no.16KG166), and the National Natural Science of Foundation of China (no. 81770612).
Compliance with ethical standards
This study was approved by the Ethics Committee of Tianjin Children’s Hospital and written informed consent for the use of clinical data and blood samples was signed by all participants.
Conflict of interest
On behalf of all authors, the corresponding author states that there is no conflict of interest.
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