Abstract
Introduction
Integrins and vascular endothelial growth factor (VEGF) are crucially involved in interaction, proliferation, migration, and survival of the cells. However, there is no report in the literature about β1 integrin and VEGF expression in heterotopic brain tissue.
Purpose
The aim of this study was to assess β1 integrin and VEGF expression in experimental brain tissue heterotopia in the lung during both fetal and neonatal periods.
Materials and methods
Twenty-four pregnant female Swiss mice were used to induce brain tissue heterotopia on the 15th gestational day. Briefly, the brain of one fetus of each dam was extracted, disaggregated, and injected into the right hemithorax of siblings. Six of these fetuses with pulmonary brain tissue implantation were collected on the 18th gestational day (group E18) and six other on the eighth postnatal day (group P8).
Results
Immunohistochemistry of the fetal trunks showed implantation of glial fibrillary acidic protein- and neuronal nuclei-positive heterotopic brain tissue, which were also positive for β1 integrin and VEGF in both groups E18 and P8.
Conclusion
These results indicate that brain tissue heterotopia during fetal and postnatal period is able to complete integration with the lung tissue as well as to induce vascular proliferation which are the necessary steps for a successful implantation.
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Fundação de Amparo à Pesquisa do Estado de São Paulo and Conselho Nacional de Desenvolvimento Científico e Tecnológico.
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Quemelo, P.R.V., Simões, D.L.C. & Peres, L.C. β1 Integrin and VEGF expression in an experimental model of brain tissue heterotopia in the lung. Childs Nerv Syst 26, 807–810 (2010). https://doi.org/10.1007/s00381-009-1053-x
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DOI: https://doi.org/10.1007/s00381-009-1053-x