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Soil disturbance and water stress interact to influence arbuscular mycorrhizal fungi, rhizosphere bacteria and potential for N and C cycling in an agricultural soil

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Abstract

The objective of this study was to determine how soil disturbance and soil water deficit alter colonisation of roots by naturally occurring arbuscular mycorrhizal (AM) fungi and rhizosphere bacteria. Soil cores were collected at the end of summer from a cropped paddock with a 5-year history of no-tillage in south-western Australia which has a Mediterranean climate. Well-watered and water-stressed treatments were maintained at 70 and 35% field capacity, respectively. AM fungal colonisation was determined using microscopy, rhizosphere bacterial community composition was assessed using barcoded PCR-amplified bacterial 16S rRNA genes, and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis of functional gene prediction was used to characterise the rhizosphere functionality for some nitrogen (N) cycling and soil carbon (C) degradation. Mycorrhizal colonisation and plant growth were reduced by disturbance under water stress but not for well-watered soil cores. The rhizosphere bacterial community composition shifted with both soil disturbance and soil moisture, and there was an interaction between them. Disturbance decreased the relative abundance of Proteobacteria and Acidobacteria and increased the relative abundance of Actinobacteria and Firmicutes in water-stressed soil. The predicted abundance of some genes involved in N reactions was negatively influenced by both disturbance and soil moisture, but the predicted abundance of C degrading predicted genes was only marginally affected. This study highlighted how soil disturbance prior to seeding can alter soil biological processes that influence plant growth and that this could be most pronounced when water is limiting.

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References

  • Abbott LK, Robson AD (1981) Infectivity and effectiveness of vesicular arbuscular mycorrhizal fungi: effect of inoculum type. Aust J Agric Res 32:631–639

    Google Scholar 

  • Abbott LK, Robson AD (1985) Formation of external hyphae in soil by four species of vesicular-arbuscular mycorrhizal fungi. New Phytol 99:245–255

    Google Scholar 

  • Ashworth AJ, DeBruyn JM, Allen FL, Radosevich M, Owens PR (2017) Microbial community structure is affected by cropping sequences and poultry litter under long-term no-tillage. Soil Biol Biochem 114:210–219

    CAS  Google Scholar 

  • Al-Kaisi MM, Yin X (2005) Tillage and crop residue effects on soil carbon and carbon dioxide emission in corn–soybean rotations. J Env 34:437–445

    CAS  Google Scholar 

  • Artursson V, Finlay RD, Jansson JK (2006) Interactions between arbuscular mycorrhizal fungi and bacteria and their potential for stimulating plant growth. Environ Microbiol 8:1–10

    CAS  PubMed  Google Scholar 

  • Augé RM (2001) Water relations, drought and vesicular-arbuscular mycorrhizal symbiosis. Mycorrhiza 11:3–42

    Google Scholar 

  • Bálint M, Bartha L, O'Hara RB, Olson MS, Otte J, Pfenninger M, Schmitt I (2015) Relocation, high-latitude warming and host genetic identity shape the foliar fungal microbiome of poplars. Mol Ecol 24:235–248

    PubMed  Google Scholar 

  • Barnard RL, Osborne CA, Firestone MK (2013) Responses of soil bacterial and fungal communities to extreme desiccation and rewetting. ISME J 7:2229–2241

    CAS  PubMed  PubMed Central  Google Scholar 

  • Barton L, Murphy DV, Butterbach-Bahl K (2013) Influence of crop rotation and liming on greenhouse gas emissions from a semi-arid soil. Ecosyst Env 167:23–32

    CAS  Google Scholar 

  • Boddington CL, Dodd JC (2000) The effect of agricultural practices on the development of indigenous arbuscular mycorrhizal fungi II Studies in experimental microcosms. Plant Soil 218:145–157

    CAS  Google Scholar 

  • Bulgarelli D, Schlaeppi K, Spaepen S, van Themaat EVL, Schulze-Lefert P (2013) Structure and functions of the bacterial microbiota of plants. Annu Rev Plant Biol 64:807–838

    CAS  PubMed  Google Scholar 

  • Cambardella CA, Elliott ET (1992) Particulate soil organic-matter changes across a grassland cultivation sequence. Soil Sci Soc Amer J 56:777–783

    Google Scholar 

  • Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N, Pena AG, Goodrich JK, Gordon JI, Huttley GA, Kelley ST, Knights D, Koenig JE, Ley RE, Lozupone CA, McDonald D, Muegge BD, Pirrung M, Reeder J, Sevinsky JR, Tumbaug PJ, Walters WA, Widmann J, Yatsunenko T, Zaneveld J, Knight R (2010) QIIME allows analysis of high-throughput community sequencing data. Nat Methods 7:335–336

    CAS  PubMed  PubMed Central  Google Scholar 

  • Caporaso JG, Lauber CL, Walters WA, Berg-Lyons D, Huntley J, Fierer N, Owens SM, Betley J, Fraser L, Bauer M, Gormley N (2012) Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms. ISME J 6:1621–1624

    CAS  PubMed  PubMed Central  Google Scholar 

  • Chávez-Romero Y, Navarro-Noya YE, Reynoso-Martínez SC, Sarria-Guzmán Y, Govaerts B, Verhulst N, Dendooven L, Luna-Guido M (2016) 16S metagenomics reveals changes in the soil bacterial community driven by soil organic C, N-fertilizer and tillage-crop residue management. Soil Tillage Res 159:1–8

    Google Scholar 

  • Cleveland CC, Nemergut DR, Schmidt SK, Townsend AR (2007) Increases in soil respiration following labile carbon additions linked to rapid shifts in soil microbial community composition. Biogeochem 82:229–240

    CAS  Google Scholar 

  • Compant S, Van Der Heijden MGA, Sessitsch A (2010) Climate change effects on beneficial plant–microorganism interactions. FEMS Microbiol Ecol 73:197–214

    CAS  PubMed  Google Scholar 

  • Diffenbaugh NS, Giorgi F (2012) Climate change hotspots in the CMIP5 global climate model ensemble. Clim Chang 114:813–822

    Google Scholar 

  • DeVries FT, Liiri ME, Bjørnlund L, Bowker MA, Christensen S, Setälä HM, Bardgett RD (2012) Land use alters the resistance and resilience of soil food webs to drought. Nat Clim Chang 2:276–280

    Google Scholar 

  • Dong W, Liu E, Yan C, Tian J, Zhang H, Zhang Y (2017) Impact of no tillage vs. conventional tillage on the soil bacterial community structure in a winter wheat cropping succession in northern China. Eur J Soil Biol 80:35–42

    Google Scholar 

  • Dubrovský M, Hayes M, Duce P, Trnka M, Svoboda M, Zara P (2014) Multi-GCM projections of future drought and climate variability indicators for the Mediterranean region. Reg Env Change 14:1907–1919

    Google Scholar 

  • Edgar RC, Haas BJ, Clemente JC, Quince C, Knight R (2011) UCHIME improves sensitivity and speed of chimera detection. Bioinform 27:2194–2200

    CAS  Google Scholar 

  • Evans DG, Miller MH (1990) The role of the external mycelial network in the effect of soil disturbance upon vesicular—arbuscular mycorrhizal colonization of maize. New Phytol 114:65–71

    PubMed  Google Scholar 

  • Fierer N, Bradford MA, Jackson RB (2007) Toward an ecological classification of soil bacteria. Ecology 88:1354–1364

    PubMed  Google Scholar 

  • Fisk LM, Barton L, Jones DL, Glanville HC, Murphy DV (2015) Root exudate carbon mitigates nitrogen loss in a semi-arid soil. Soil Biol Biochem 88:380–389

    CAS  Google Scholar 

  • Frank D, Reichstein M, Bahn M, Thonicke K, Frank D, Mahecha MD, Pete S, Velde M, Vicca S, Babst F, Beer C, Buchmann N, Canadell JG, Ciais P, Cramer W, Ibrom A, Miglietta F, Poulter B, Rammig A, Seneviratne S, Walz A, Wattenbach M, Zavala MA, Zscheischler J (2015) Effects of climate extremes on the terrestrial carbon cycle: concepts, processes and potential future impacts. Glob Chang Biol 21:2861–2880

    PubMed  PubMed Central  Google Scholar 

  • Fu L, Ruan Y, Tao C, Li R, Shen Q (2016) Continuous application of bioorganic fertilizer induced resilient culturable bacteria community associated with banana Fusarium wilt suppression. Sci Rep 6:27731

    CAS  PubMed  PubMed Central  Google Scholar 

  • Fuchslueger L, Bahn M, Fritz K, Hasibeder R, Richter A (2014) Experimental drought reduces the transfer of recently fixed plant carbon to soil microbes and alters the bacterial community composition in a mountain meadow. New Phytol 201:916–927

    CAS  PubMed  Google Scholar 

  • Gihring TM, Green SJ, Schadt CW (2011) Massively parallel rRNA gene sequencing exacerbates the potential for biased community diversity comparisons due to variable library sizes. Environ Microbiol 14:285–290

    PubMed  Google Scholar 

  • Giovannetti M, Mosse B (1980) An evaluation of techniques for measuring vesicular arbuscular mycorrhizal infection in roots. New Phytol 84:489–500

    Google Scholar 

  • Giorgi F (2006) Climate change hot-spots. Geophys Res Lett 33

  • Gleeson D, Mathes F, Farrell M, Leopold M (2016) Environmental drivers of soil microbial community structure and function at the Avon River critical zone observatory. Sci Total Environ 571:1407–1418

    CAS  PubMed  Google Scholar 

  • Glick BR (1995) The enhancement of plant growth by free-living bacteria. Can J Microbiol 41:109–117

    CAS  Google Scholar 

  • Good IJ (1953) The population frequencies of species and the estimation of population parameters. Biometrika 40:237–264

    Google Scholar 

  • Green SJ, Inbar E, Michel FC, Hadar Y, Minz D (2006) Succession of bacterial communities during early plant development: transition from seed to root and effect of compost amendment. Appl Environ Microbiol 72:3975–3983

    CAS  PubMed  PubMed Central  Google Scholar 

  • Green SJ, Michel FC Jr, Hadar Y, Minz D (2007) Contrasting patterns of seed and root colonization by bacteria from the genus Chryseobacterium and from the family Oxalobacteraceae. The ISME J 1:291–299

    CAS  PubMed  Google Scholar 

  • Grice EA, Segre JA (2012) The human microbiome: our second genome. Annu Rev Genomics Hum Genet 13:151–170

    CAS  PubMed  PubMed Central  Google Scholar 

  • Hayden HL, Drake J, Imhof M, Oxley AP, Norng S, Mele PM (2010) The abundance of nitrogen cycle genes amoA and nifH depends on land-uses and soil types in south-eastern Australia. Soil Biol Biochem 42:1774–1783

    CAS  Google Scholar 

  • Hart MM, Zaitsoff PD, van der Heyde M, Pither J (2016) Testing life history and trait-based predictions of AM fungal community assembly. Pedobiologia 59:203–213

    Google Scholar 

  • van der Heyde M, Ohsowski B, Abbott LK, Hart M (2017) Arbuscular mycorrhizal fungus responses to disturbance are context-dependent. Mycorrhiza 27:431–440

    PubMed  Google Scholar 

  • Helgason BL, Walley FL, Germida JJ (2010) No-till soil management increase microbial biomass and later community profiles in soil aggregates. Appl Soil Ecol 46:390–397

    Google Scholar 

  • Holmes AJ, Bowyer J, Holley MP, O'Donoghue M, Montgomery M, Gillings MR (2000) Diverse, yet-to-be-cultured members of the Rubrobacter subdivision of the Actinobacteria are widespread in Australian arid soils. FEMS Microbiol Ecol 33:111–120

    CAS  PubMed  Google Scholar 

  • Hoyle FC, Murphy DV (2006) Seasonal changes in microbial function and diversity associated with stubble retention versus burning. Soil Res 44:407–423

    Google Scholar 

  • Isbell R (2002) The Australian Soil Classification. CSIRO Publishing, Canberra

    Google Scholar 

  • Jasper DA, Abbott LK, Robson AD (1989) Soil disturbance reduces the infectivity of external hyphae of vesicular—arbuscular mycorrhizal fungi. New Phytol 112:93–99

    Google Scholar 

  • Jasper DA, Abbott LK, Robson AD (1991) The effect of soil disturbance on vesicular—arbuscular mycorrhizal fungi in soils from different vegetation types. New Phytol 118:471–476

    Google Scholar 

  • Jenkins SN, Rushton SP, Lanyon CV, Whiteley AS, Waite IS, Brookes PC, Kemmitt S, Evershed RP, O’Donnell AG (2010) Taxon-specific responses of soil bacteria to the addition of low level C inputs. Soil Biol Biochem 42:1624–1631

    CAS  Google Scholar 

  • Jenkins SN, Waite IS, Blackburn A, Husband R, Rushton SP, Manning DC, O’Donnell AG (2009) Actinobacterial community dynamics in long term managed grasslands. Antonie Van Leeuwenhoek 95:319–334

    PubMed  Google Scholar 

  • Jones DL, Nguyen C, Finlay RD (2009) Carbon flow in the rhizosphere: carbon trading at the soil–root interface. Plant Soil 321:5–33

    CAS  Google Scholar 

  • Keil D, Niklaus PA, von Riedmatten LR, Boeddinghaus RS, Dormann CF, Scherer-Lorenzen M et al (2015) Effects of warming and drought on potential N2O emissions and denitrifying bacteria abundance in grasslands with different land-use. FEMS Microbiol Ecol 91:7

    Google Scholar 

  • Knopke P, O'Donnell V, Shepherd A (2000) Productivity growth in the Australian grains industry. ABARE Res Report, (2000.1)

  • Kwon MJ, Haraguchi A, Kang H (2013) Long-term water regime differentiates changes in decomposition and microbial properties in tropical peat soils exposed to the short-term drought. Soil Biol Biochem 60:33–44

    CAS  Google Scholar 

  • Langille MG, Zaneveld J, Caporaso JG, McDonald D, Knights D, Reyes JA, Clemente JC, Burkepile DE, Vega Thurber RL, Knight R, Beiko RG, Huttenhower C (2013) Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences. Nat Biotechnol 31:814–821

    CAS  PubMed  PubMed Central  Google Scholar 

  • Llewellyn RS, D’Emden FH, Kuehne G (2012) Extensive use of no-tillage in grain growing regions of Australia. Field Crop Res 132:204–212

    Google Scholar 

  • Manzoni S, Schimel JP, Porporato A (2012) Responses of soil microbial communities to water stress: results from a meta-analysis. Ecology 93:930–938

    PubMed  Google Scholar 

  • McDonald RIR, Speight J, Walker J, Hopkins M (1998) Australian Soil and Land Survey Field Handbook. (CSIRO Publishing, Canberra)

  • Mehlich A (1984) Mehlich 3 soil test extractant: a modification of Mehlich 2 extractant. Commun Soil Sci Plant Anal 15:1409–1416

    CAS  Google Scholar 

  • Mickan BS, Abbott LK, Stefanova K, Solaiman ZM (2016) Interactions between biochar and mycorrhizal fungi in a water-stressed agricultural soil. Mycorrhiza 26:565–574

    CAS  PubMed  Google Scholar 

  • Mickan BS, Abbott LK, Fan J, Hart MM, Siddique KH, Solaiman ZM, Jenkins SN (2017) Application of compost and clay under water-stressed conditions influences functional diversity of rhizosphere bacteria. Biol Fertil Soils 54:55–70

    Google Scholar 

  • Mickan BS (2014) Mechanisms for alleviation of plant water stress involving arbuscular mycorrhizas. In: Solaiman ZM, Abbott LK, Ajit V (Ed); Mycorrhizal Fungi: In Use in Sustainable Agriculture and Land Restoration. Springer Berlin Heidelberg pp. 225–239

  • Mori H, Maruyama F, Kato H, Toyoda A, Dozono A, Ohtsubo Y, Nagata Y, Fujiyama A, Tsuda M, Kurokawa K (2014) Design and experimental application of a novel non-degenerate universal primer set that amplifies prokaryotic 16S rRNA genes with a low possibility to amplify eukaryotic rRNA genes. DNA Res 21:217–227

    CAS  PubMed  Google Scholar 

  • Moriondo M, Giannakopoulos C, Bindi M (2011) Climate change impact assessment: the role of climate extremes in crop yield simulation. Clim Chang 104:679–701

    Google Scholar 

  • Moyano FE, Manzoni S, Chenu C (2013) Responses of soil heterotrophic respiration to moisture availability: an exploration of processes and models. Soil Biol Biochem 59:72–85

    CAS  Google Scholar 

  • Neumann E, Schmid B, Römheld V, George E (2010) Extraradical development and contribution to plant performance of an arbuscular mycorrhizal symbiosis exposed to complete or partial rootzone drying. Mycorrhiza 20:13–23

    Google Scholar 

  • Newman EI (1966) A method of estimating the total length of root in a sample. J Appl Ecol 3:139–145

    Google Scholar 

  • Ofek M, Hadar Y, Minz D (2012) Ecology of root colonizing Massilia (Oxalobacteraceae). PLoS One 7:e40117

    CAS  PubMed  PubMed Central  Google Scholar 

  • Oksanen J, Blanchet FG, Kindt R, Legendre P, Minchin PR, O’Hara RB, Simpson GL, Solymos P, Stevens MHH, Wagner H, (2013) Package ‘vegan’. Community Ecology Package, version, 2(9)

  • Pascault N, Ranjard L, Kaisermann A, Bachar D, Christen R, Terrat S et al (2013) Stimulation of different functional groups of bacteria by various plant residues as a driver of soil priming effect. Ecosyst 16:810–822

    CAS  Google Scholar 

  • Pinheiro J, Bates D, DebRoy S, Sarkar D & Team RC (2017) nlme: Linear and nonlinear mixed effects models (R package version 3.1–128, 2016). R software

  • Pruesse E, Quast C, Knittel K, Fuchs BM, Ludwig W, Peplies J, Glöckner FO (2007) SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB. Nucleic Acids Res 35:7188–7196

    CAS  PubMed  PubMed Central  Google Scholar 

  • Rayment G, Lyons D (2011) Soil chemical methods—Australasia. CSIRO Publishing, Melbourne

    Google Scholar 

  • Ruiz-Lozano JM, Porcel R, Bárzana G, Azcon R, Aroca R (2012) Contribution of arbuscular mycorrhizal symbiosis to plant drought tolerance: state of the art. In: Aroca P (ed) Plant responses to drought stress. Springer, Berlin, pp 335–362

    Google Scholar 

  • R Core Team (2015) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria URL http://www.R-project.org/

    Google Scholar 

  • Ruth B, Khalvati M, Schmidhalter U (2011) Quantification of mycorrhizal water uptake via high-resolution on-line water content sensors. Plant Soil 342:459–468

    CAS  Google Scholar 

  • Scheublin TR, Sanders IR, Keel C, van der Meer JR (2010) Characterisation of microbial communities colonising the hyphal surfaces of arbuscular mycorrhizal fungi. The ISME J 4:752–763

    PubMed  Google Scholar 

  • Schimel J, Balser TC, Wallenstein M (2007) Microbial stress-response physiology and its implications for ecosystem function. Ecology 88:1386–1394

    PubMed  Google Scholar 

  • Schloss PD, Gevers D, Westcott SL (2011) Reducing the effects of PCR amplification and sequencing artifacts on 16S rRNA-based studies. PLoS One 6:e27310

    CAS  PubMed  PubMed Central  Google Scholar 

  • Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, Lesniewski RA, Oakley BB, Parks DH, Robinson CJ, Sahl JW, Stres B, Thallinger GG, Van Horn DJ, Weber CF (2009) Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol 75:7537–7541

    CAS  PubMed  PubMed Central  Google Scholar 

  • Schöler A, Jacquiod S, Vestergaard G, Schulz S, Schloter M (2017) Analysis of soil microbial communities based on amplicon sequencing of marker genes. Biol Fertil Soils 53:485–489

    Google Scholar 

  • Silva-Olaya AM, Cerri CEP, La Scala N Jr, Dias CTS, Cerri CC (2013) Carbon dioxide emissions under different soil tillage systems in mechanically harvested sugarcane. Environ Res Lett 8:015014

    Google Scholar 

  • Smith SE, Read DJ (2008) Mycorrhizal symbiosis, 3rd edn. Academic, New York

    Google Scholar 

  • So HB, Grabski A, Desborough P (2009) The impact of 14 years of conventional and no-till cultivation on the physical properties and crop yields of a loam soil at Grafton NSW, Australia. Soil Tillage Res 104:180–184

    Google Scholar 

  • Stevenson BA, Hunter DW, Rhodes PL (2014) Temporal and seasonal change in microbial community structure of an undisturbed, disturbed, and carbon-amended pasture soil. Soil Biol Biochem 75:175–185

    CAS  Google Scholar 

  • Taketani RG, Lançoni MD, Kavamura VN, Durrer A, Andreote FD, Melo IS (2016) Dry season constrains bacterial phylogenetic diversity in a semi-arid rhizosphere system. Microb Ecol 73:153–161

    PubMed  Google Scholar 

  • Vestergaard G, Schulz S, Schöler A, Schloter M (2017) Making big data smart—how to use metagenomics to understand soil quality. Biol Fertil Soils 53:479–484

    Google Scholar 

  • Verzeaux J, Roger D, Lacoux J, Nivelle E, Adam C, Habbib H, Hirel B, Dubois F, Tetu T (2016) In winter wheat, no-till increases mycorrhizal colonization thus reducing the need for nitrogen fertilization. Agronomy 6:38

    Google Scholar 

  • White JR, Nagarajan N, Pop M (2009) Statistical methods for detecting differentially abundant features in clinical metagenomic samples. PLoS Comp Biol 5:e1000352

    Google Scholar 

  • Whiteley AS, Jenkins S, Waite I, Kresoje N, Payne H, Mullan B, Allcock R, O'Donnell A (2012) Microbial 16S rRNA ion tag and community metagenome sequencing using the ion torrent (PGM) platform. J Microbiol Methods 91:80–88

    CAS  PubMed  Google Scholar 

  • Zenova GM, Gryadunova AA, Doroshenko EA, Likhacheva AA, Sudnitsyn II, Pochatkova TN, Zvyagintsev DG (2007) Influence of moisture on the vital activity of actinomycetes in a cultivated low-moor peat soil. Eurasian Soil Sci 40:560–564

    Google Scholar 

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Acknowledgements

We thank Peter Nixon for allowing us to collect soil from his farm at Moora, Western Australia; Ian Waite for his valuable laboratory assistance and Russell Martin and Brenton Leske for assisting in the glasshouse.

Funding

This research was funded by an Australian Post Graduate Scholarship, and The University of Western Australia Safety net top up.

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  1. UWA School of Agriculture and Environment (M079), The University of Western Australia, Perth, WA, 6009, Australia

    Bede S. Mickan, Lynette K. Abbott, Zakaria M. Solaiman, Falko Mathes & Sasha N. Jenkins

  2. The UWA Institute of Agriculture (M082), The University of Western Australia, Perth, WA, 6009, Australia

    Bede S. Mickan, Lynette K. Abbott, Zakaria M. Solaiman, Kadambot H. M. Siddique & Sasha N. Jenkins

  3. Richgro Garden Products, 203 Acourt Rd., Jandakot, Western Australia, 6164, Australia

    Bede S. Mickan

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Mickan, B.S., Abbott, L.K., Solaiman, Z.M. et al. Soil disturbance and water stress interact to influence arbuscular mycorrhizal fungi, rhizosphere bacteria and potential for N and C cycling in an agricultural soil. Biol Fertil Soils 55, 53–66 (2019). https://doi.org/10.1007/s00374-018-1328-z

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