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Diazotrophyc rhizobacteria isolated from sugarcane can release amino acids in a synthetic culture medium

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Abstract

The active release of amino acids by diazotrophic rhizobacteria into the natural environment or under in vitro conditions is poorly described in the literature. This capacity could be an important trait in the plant–bacteria interaction and in plant growth promotion. The ability of releasing amino acids into a medium free of N-combined was studied in cultures of five diazotrophic genera, Beijerinckia, Burkholderia, Enterobacter, Klebsiella, and Pseudomonas, isolated from the sugarcane rhizosphere. Eleven different amino acids were excreted into the culture media by 40% of the isolates. The highest amino acid diversities were found in Beijerinckia (ICBR 177) and Enterobacter (ICBR 200). The highest quantities were excreted by Beijerinckia (ICBR 177), Enterobacter (ICBR 200), Pseudomonas (ICBR 56), and Klebsiella (ICBR 183). The production of amino acids by rhizobacteria may play an important role in the growth of plants and might also have a direct application in agricultural technologies. The release of amino acids could explain one of the positive effects of plant growth-promoting bacteria and encourage further studies of this subject.

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References

  • Barbosa HR, Rodrigues MFA, Campos CC, Chaves ME, Nunes I, Juliano Y, Novo NF (1995) Counting of viable cluster-forming and non cluster-forming bacteria: a comparison between the drop and the spread methods. J Microbiol Meth 22:39–50

    Article  Google Scholar 

  • Biggs IM (2003) An investigation of sugarcane nitrogen physiology: sources, uptake and metabolism. Dissertation. School of Life Sciences, The University of Queensland, Brisbane, Australia, p 139

  • Cattelán AJ, Hartel PG, Fuhrmann JJ (1999) Screening for plant growth promoting rhizobacteria to promote early soybean growth. Soil Sci Soc Am J 63:1670–1680

    Article  Google Scholar 

  • Chapin FS, Moilanem L, Kiellander K (1993) Preferential use of organic nitrogen for growth by a non-mycorrhizal arctic sedge. Nature 361:150–153

    Article  CAS  Google Scholar 

  • Döbereiner J (1980) Forages grasses and grain crops. In: Bergersen FJ (ed) Methods for evaluating biological nitrogen fixation. Willey, New York, pp 535–555

    Google Scholar 

  • Döbereiner J (1997) Biological nitrogen fixation in the tropics: social and economic contributions. Soil Biol Biochem 29:771–774

    Article  Google Scholar 

  • Eggeling L, Sahm H (2003) New ubiquitous translocators: amino acid export by Corynebacterium glutamicum and Escherichia coli. Arch Microbiol 180:155–160

    Article  PubMed  CAS  Google Scholar 

  • González-Lopez J, Rodelas B, Pozo C, Salmeron-Lopez V, Martınez-Toledo MV, Salmerón-Lopez V (2005) Liberation of amino acids by heterotrophic nitrogen fixing bactéria. Amino Acids 28:363–367

    Article  PubMed  Google Scholar 

  • Kato T (2006) Major nitrogen compounds transported in xylem vessels from roots to top in Citrus trees. Physiol Plant 52:275–279

    Article  Google Scholar 

  • Kielland K (1994) Amino acid absorption by arctic plants: implications for plant nutrition and nitrogen cycling. Ecology 75:2373–2383

    Article  Google Scholar 

  • Liba CM, Ferrara FIS, Manfio GP, Fantinatti-Garboggini F, Albuquerque RC, Pavan C, Ramos PL, Moreira-Filho CA, Barbosa HR (2006) Nitrogen-fixing chemoorganotrophic bacteria isolated from cyanobacteria-deprived lichens and their ability to solubilise phosphate and to release amino acids and phytormones. J Appl Microbiol 101:1076–1086

    Article  PubMed  CAS  Google Scholar 

  • Lipson DA, Näsholm T (2001) The unexpected versatility of plants: organic nitrogen use and availability in terrestrial ecosystems. Oecologia 128:305–316

    Article  Google Scholar 

  • Lipson DA, Raab TK, Schmidt SK, Monson RK (1999) Variation in competitive abilities of plants and microbes for specific amino acids. Biol Fert Soils 29:257–261

    Article  CAS  Google Scholar 

  • Loiret FG, Grimm B, Hajirezaei MR, Kleiner D, Ortega E (2009) Inoculation of sugarcane with Pantoea sp. increases amino acid contents in shoot tissues; serine, alanine, glutamine and asparagine permit concomitantly ammonium excretion and nitrogenase activity of the bacterium. J Plant Physiol 166:1152–1161

    Article  PubMed  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Näsholm T, Kielland K, Ganeteg U (2009) Uptake of organic nitrogen by plants. New Phytol 182:31–48

    Article  PubMed  Google Scholar 

  • Neff JC, Chapin FS, Vitousek FSPM (2003) Breaks in the cycle: dissolved organic nitrogen in terrestrial ecosystems. Front Ecol Environ 1:205–211

    Article  Google Scholar 

  • Olivares FL, Baldani VLD, Reis VM, Baldani JI, Döbereiner J (1996) Occurrence of the endophytic diazotrophs Herbaspirillum spp. in roots, stems and leaves predominantly of graminea. Biol Fertil Soils 21:197–200

    Article  Google Scholar 

  • Owen AG, Jones DL (2001) Competition for amino acids between wheat roots and rhizosphere microorganisms and role of amino acids in plant N acquisition. Soil Biol Biochem 33:651–657

    Article  CAS  Google Scholar 

  • Pariona-Llanos R, Ferrara FIS, Soto HH, Barbosa HR (2010) Influence of organic fertilization on the number of culturable diazotrophic endophytic bacteria isolated from sugarcane. Eur J Soil Biol 46:387–393

    Article  Google Scholar 

  • Pati BR, Sengupta S, Chandra AK (1994) Studies on the amino acids released by phyllosphere diazotrophic bacteria. Microbiol Res 149:287–290

    CAS  Google Scholar 

  • Persson J, Näsholm T (2001) Amino acid uptake: a widespread ability among boreal forest plants. Ecol Lett 4:434–438

    Article  Google Scholar 

  • Reeve JR, Smith JL, Carpenter-Boggs L, Reganold JP (2009) Glycine, nitrate and ammonioum uptake by classic and modern wheat varieties in a short-term microcosm study. Biol Fertil Soils 45:723–732

    Article  CAS  Google Scholar 

  • Ruschel AP, Vose PB (1984) Biological nitrogen fixation in sugar cane. In: Subba R (ed) Current developments in biological nitrogen fixation. Edward Arnold (Publishers) Ltd, London, pp 219–235

    Google Scholar 

  • Salmeron-Lopez V, Martinez-Toledo MV, Salmeron-Miron V, Pozo C, González-Lopez J (2004) Production of amino acids by Rhizobium, Mesorhizobium and Sinorhizobium strains in chemically defined media. Amino Acids 27:169–174

    Article  PubMed  CAS  Google Scholar 

  • Seefeldt LC, Dance IG, Dean DR (2004) Substrate interactions with nitrogenase: Fe versus Mo. Biochem 43:1401–1409

    Article  CAS  Google Scholar 

  • Subasinghe R (2007) Effect of external nitrogen and potassium supply on xylem sap composition of sugarcane. J Plant Nutr 30:187–201

    Article  CAS  Google Scholar 

  • Taiz L, Zeiger E (2002) Plant physiology. 3ed. Sinauer Associates. Sunderland, MA, USA, p 690

    Google Scholar 

  • Thuler DS, Floh EIS, Handro W, Barbosa HR (2003a) Beijerinckia derxii releases plant growth regulators and amino acids on synthetic media independent of nitrogenase activity. J Appl Microbiol 95:799–806

    Article  PubMed  CAS  Google Scholar 

  • Thuler DS, Floh EIS, Handro W, Barbosa HR (2003b) Plant growth regulators and amino acids released by Azospirillum sp. in chemically defined media. Lett Appl Microbiol 37:174–178

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

The authors wish to thank FAPESP for grants, Fazenda São Francisco for kindly providing the sugarcane samples and Dr. Marcel Bouquet for volunteering his technical and linguistic skills to the English version of this paper.

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Authors and Affiliations

  1. Departamento de Microbiologia, Laboratório de Fisiologia de Microrganismos, Instituto de Ciências Biomedicas, Universidade de São Paulo, Avenida Prof. Lineu Prestes, 1374, São Paulo, CEP 05508-000, Brazil

    Zilda M. de Oliveira, Felipe I. S. Ferrara & Heloiza R. Barbosa

  2. Departamento de Botânica, Laboratório de Biologia Celular de Plantas, Instituto de Biociências, Universidade de São Paulo, Rua do Matão, 277, São Paulo, CEP 05508-090, Brazil

    Eny I. S. Floh

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  1. Zilda M. de Oliveira
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  2. Eny I. S. Floh
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  3. Felipe I. S. Ferrara
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  4. Heloiza R. Barbosa
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Correspondence to Felipe I. S. Ferrara.

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de Oliveira, Z.M., Floh, E.I.S., Ferrara, F.I.S. et al. Diazotrophyc rhizobacteria isolated from sugarcane can release amino acids in a synthetic culture medium. Biol Fertil Soils 47, 957–962 (2011). https://doi.org/10.1007/s00374-011-0557-1

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  • DOI: https://doi.org/10.1007/s00374-011-0557-1

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