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Sources of Ca2+ for contraction of the heart tube of Tenebrio molitor (Coleoptera: Tenebrionidae)

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Abstract

Insect and vertebrate hearts share the ability to generate spontaneously their rhythmic electrical activity, which triggers the fluid-propelling mechanical activity. Although insects have been used as models in studies on the impact of genetic alterations on cardiac function, there is surprisingly little information on the generation of the inotropic activity in their hearts. The main goal of this study was to investigate the sources of Ca2+ for contraction in Tenebrio molitor hearts perfused in situ, in which inotropic activity was assessed by the systolic variation of the cardiac luminal diameter. Increasing the pacing rate from 1.0 to 2.5 Hz depressed contraction amplitude and accelerated relaxation. To avoid inotropic interference of variations in spontaneous rate, which have been shown to occur in insect heart during maneuvers that affect Ca2+ cycling, experiments were performed under electrical pacing at near-physiological rates. Raising the extracellular Ca2+ concentration from 0.5 to 8 mM increased contraction amplitude in a manner sensitive to L-type Ca2+ channel blockade by D600. Inotropic depression was observed after treatment with caffeine or thapsigargin, which impair Ca2+ accumulation by the sarcoplasmic reticulum (SR). D600, but not inhibition of the sarcolemmal Na+/Ca2+ exchanger by KB-R7943, further depressed inotropic activity in thapsigargin-treated hearts. From these results, it is possible to conclude that in T. molitor heart, as in vertebrates: (a) inotropic and lusitropic activities are modulated by the heart rate; and (b) Ca2+ availability for contraction depends on both Ca2+ influx via L-type channels and Ca2+ release from the SR.

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Abbreviations

[Ca2+]o :

Extracellular Ca2+ concentration

FS:

Fractional shortening

HR:

Heart rate

NCX:

Na+–Ca2+ exchanger

SR:

Sarcoplasmic reticulum

T 0.5−rel :

Half-time of relaxation

T pk :

Time to peak contraction amplitude

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Acknowledgements

We are indebted to Ms. Elizângela S. Oliveira and Mr. Renato S. Moura (Center for Biomedical Engineering/UNICAMP) for technical support. This study was supported by the Conselho Nacional de Pesquisa e Desenvolvimento (CNPq), Grants no 302996/2011-7 (JWMB), no 155508/2010-5 and no 163911/2012-6 (AFN).

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Authors and Affiliations

  1. Department of Biomedical Engineering, School of Electrical and Computer Engineering, and Center for Biomedical Engineering, University of Campinas (UNICAMP), R. Alexander Fleming, 163, Cidade Universitária Zeferino Vaz, Campinas, SP, 13083-881, Brazil

    Arnaldo Fim Neto, Rosana A. Bassani, Pedro X. de Oliveira & José W. M. Bassani

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  1. Arnaldo Fim Neto
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  2. Rosana A. Bassani
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  3. Pedro X. de Oliveira
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  4. José W. M. Bassani
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Correspondence to Arnaldo Fim Neto.

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Communicated by G. Heldmaier.

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Fim Neto, A., Bassani, R.A., de Oliveira, P.X. et al. Sources of Ca2+ for contraction of the heart tube of Tenebrio molitor (Coleoptera: Tenebrionidae). J Comp Physiol B 188, 929–937 (2018). https://doi.org/10.1007/s00360-018-1183-0

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