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Journal of Comparative Physiology B

, Volume 188, Issue 3, pp 541–551 | Cite as

Changes in thermoregulation and monoamine release in freely moving rats during cold exposure and inhibition of the ventromedial, dorsomedial, or posterior hypothalamus

  • Takayuki Ishiwata
  • Benjamin N. Greenwood
Original Paper

Abstract

The hypothalamus is critical for regulating thermogenesis, but the role of monoamines in specific hypothalamic subregions in thermogenesis is not thoroughly established. The purpose of this study was to confirm changes of body temperature (T b) and thermoregulatory parameters upon inhibition of neural activity in hypothalamic subregions in freely moving rats. In addition, the pattern of monoamine release in these nuclei was measured during active thermoregulation using microdialysis. Tetrodotoxin (TTX) was perfused into the ventromedial hypothalamus (VMH), dorsomedial hypothalamus (DMH), or posterior hypothalamus (PH) at two different ambient temperatures (5 or 23 °C). Using telemetry, we continuously measured the T b and the heart rate (HR) as an index of heat production as well as locomotor activity (Act). Tail skin temperature (T tail) was also continuously measured as an index of heat loss. Although the perfusion of TTX into hypothalamic subregions had no effect on any of the measured thermoregulatory parameters at an ambient temperature of 23 °C, it induced significant T b decrease under cold conditions only when perfused into the DMH and the PH. In contrast, the HR decreased only after perfusion of TTX into the PH during cold conditions, while the T tail and Act remained unchanged. Serotonin (5-HT) in the DMH and dopamine (DA) metabolite 3,4-Dihydroxyphenylacetic acid in the PH, but not noradrenaline, increased significantly during exposure to cold temperatures. Our results indicate that the DMH and the PH, but not the VMH, are particularly involved in heat production under cold conditions. In addition, 5-HT in the DMH and DA in the PH may be involved in thermogenesis.

Keywords

Dorsomedial hypothalamus Freely moving rats Microdialysis Posterior hypothalamus Thermoregulation Ventromedial hypothalamus 

Abbreviations

5-HT

Serotonin

5-HIAA

5-Hydroxyindoleacetic acid

Ad

Adrenaline

Act

Locomotor activity

DA

Dopamine

DMH

Dorsomedial hypothalamus

DOPAC

3,4-Dihydroxyphenylacetic acid

HPLC

High-performance liquid chromatography

HR

Heart rate

HVA

Homovanillic acid

IBAT

Interscapular brown adipose tissue

NA

Noradrenaline

PH

Posterior hypothalamus

PO/AH

Preoptic area and anterior hypothalamus

PRV

Pseudorabies virus

Tb

Body temperature

Ttail

Tail skin temperature

TTX

Tetrodotoxin

VMH

Ventromedial hypothalamus

Notes

Acknowledgements

The present study was partly supported by JSPS KAKENHI Grant Number JP23700684 (T.I.). We thank Drs. Yasutsugu Aihara (Tokyo Metropolitan University), Hiroshi Hasegawa (Hiroshima University), and Takehito Saito (Kyoei University) for valuable discussion throughout the study.

Compliance with ethical standards

Conflict of interest

The authors of the present study declare no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  1. 1.Graduate School of Community and Human ServicesRikkyo UniversityNiizaJapan
  2. 2.Department of Psychology, College of Liberal Arts and SciencesUniversity of Colorado DenverDenverUSA

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