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Development of ABA Antagonists to Overcome ABA- and Low Temperature-Induced Inhibition of Seed Germination in Canola, Lentil, and Soybean

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Abstract

ABA antagonists have potential application as growth regulators to improve germination and seedling growth at low temperatures for oilseeds and pulses grown in regions with short seasons such as those in western Canada. Towards development of practical ABA antagonists, a series of 3′-substituted ABA analogs were synthesized and screened in seed germination assays in canola (Brassica napus), lentil (Lens culinaris), and soybean (Glycine max) at low temperature and in overcoming exogenous ABA. The most promising analog, ABA 1009, was selected for further germination testing of dose responses in canola, lentil, and soybean. Analog ABA 1009 at 100 µM was effective in overcoming ABA (10 µM)-induced inhibition for canola, lentil, and soybean germination at ambient temperature, and also promoted germination at low temperature for canola (5 °C).

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Abbreviations

ABA:

Abscisic acid

PYL:

Pyrabactin resistance1-like ABA receptor

PP2C:

Type 2C protein phosphatase

NRCC:

National Research Council of Canada

AAFC:

Agriculture and Agri-Food Canada

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Acknowledgements

This research was undertaken thanks in part to funding from the Canada First Research Excellence Fund (project 347165), by Agriculture Development Fund (ADF) from Government of Saskatchewan (project 20150111), a research grant from Saskatchewan Pulse Growers (project BRE1520). We thank Dr. Sally Vail AAFC for providing canola cv. Excel seeds and Dr. Steve Robinson AAFC for his guidance on the canola pouch germination assays.

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Authors and Affiliations

  1. Department of Plant Sciences, University of Saskatchewan, Saskatoon, SK, Canada

    Dongyan Song, Junsheng Zhou & Bunyamin Tar’an

  2. Department of Chemistry, University of Saskatchewan, Saskatoon, SK, Canada

    Leon Lai, Idralyn Alarcon & Suzanne Abrams

Authors
  1. Dongyan Song
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  2. Junsheng Zhou
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  3. Leon Lai
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  4. Idralyn Alarcon
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  5. Bunyamin Tar’an
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  6. Suzanne Abrams
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Correspondence to Suzanne Abrams.

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With the exception of S. Abrams who has shares in ABAzyne BioSciences, the authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Song, D., Zhou, J., Lai, L. et al. Development of ABA Antagonists to Overcome ABA- and Low Temperature-Induced Inhibition of Seed Germination in Canola, Lentil, and Soybean. J Plant Growth Regul 39, 1403–1413 (2020). https://doi.org/10.1007/s00344-019-10036-9

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  • DOI: https://doi.org/10.1007/s00344-019-10036-9

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