Lycoris is a genus of plants with flowering bulbs that have high ornamental, medicinal, and ecological value. The slow growth of Lycoris bulbs under natural conditions is a constraint to the Lycoris industry. Thus, accelerating the development of Lycoris bulbs is extremely important. Starch provides the energy and material basis for bulbs to enlarge. Previous studies have shown reduced starch metabolism capacity in Lycoris bulbs when dormant, but changes within bulbs during the vegetative growth stage remain poorly understood. In our present study, four groups of developing Lycoris radiata bulbs during the vegetative growth phase and with the same genetic background were created and used to investigate changes in carbohydrate content, starch synthesis, metabolic enzyme activity, and the gene expression patterns of several genes encoding these enzymes. Increases in enzyme activity during starch synthesis, especially for AGPase, were observed and correlated with the expression levels of the AGPase genes. Starch levels initially increased and then declined. This may be due to the increasing activity of amylase, which resulted in a rapid increase in sugar concentration during bulb development. We also detected changes in starch synthesis and metabolism from different layers of the bulbs during development, with increases in starch synthesis and starch degradation activity being more significant in the middle scales. The sugar within the middle scales may be transported to the inner scales, which increases the inner scale sugar content, promotes the expression levels of CycD genes, and accelerates cell division during bulb development.
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Chang L, Xiao YM, She LF, Xia YP (2013) Analysis of gene expression and enzyme activities related to starch metabolism in Lycoris sprengeri bulbs of different sizes. Sci Hortic 161:118–124
Francis D (2007) The plant cell cycle 15 years on. New Phytol 174(2):261–278
Goietsenoven GV, Andolfi A, Lallemand B, Cimmino A, Lamoral-Theys D, Gras T (2010) Amaryllidaceae alkaloids belonging to different structural subgroups display activity against apoptosis-resistant cancer cells. J Nat Prod 3:1223–1237
He Q, Shen Y, Wang M, Huang M, Yang R, Zhu S, Wu R (2011) Natural variation in petal color in Lycoris longituba revealed by anthocyanin components. PLoS ONE 6(8):e22098
Inze D (2005) Green light for the cell cycle. EMBO J 24(4):657–662
Janssen BJ, Thodey K, Schaffer RJ, Alba R, Balakrishnan L, Bishop R, Bowen JH, Crowhurst RN, Gleave AP, Ledger S, McArtney S, Pichler FB, Snowden KC, Ward S (2008) Global gene expression analysis of apple fruit development from the floral bud to ripe fruit. BMC Plant Biol 8(1):16
Jin Z (2009) Amaryllidaceae and sceletium alkaloids. Nat Prod Rep 26:363–381
Kang G, Liu G, Peng X, Wei L, Wang C, Zhu Y, Guo T (2013) Increasing the starch content and grain weight of common wheat by overexpression of the cytosolic AGPase large subunit gene. Plant Physiol Biochem 73:93–98
Li N, Zhang S, Zhao Y, Li B, Zhang J (2011) Over-expression of AGPase genes enhances seed weight and starch content in transgenic maize. Planta 233(2):241–250
Li X, Wang C, Cheng J, Zhang J, da Silva JA, Liu X, Duan X, Li T, Sun H (2014) Transcriptome analysis of carbohydrate metabolism during bulblet formation and development in Lilium davidii var. unicolor. BMC Plant Biol 14:358
Lytovchenko A, Fernie AR (2002) Carbon assimilation and metabolism in potato leaves deficient in plastidial phosphoglucomutase. Planta 215(5):802–811
Ma R, Xu S, Zhao Y, Xia B, Wang R (2016) Selection and validation of appropriate reference genes for quantitative real-time PCR analysis of gene expression in Lycoris aurea. Front Plant Sci 7:536
Miller WB, Langhans RW (1990) Low temperature alters carbohydrate metabolism in Easter Lily bulbs. HortSciences 25(4):463–465
Nagata T, Saitou K (2009) Regulation of expression of d3-type cyclins and adp-glucose pyrophosphorylase genes by sugar, cytokinin and aba in sweet potato (ipomoea batatas lam.). Plant Prod Sci 12(4):434–442
Nakkanong K, Yang JH, Zhang MF (2012) Starch accumulation and starch related genes expression in novel inter-specific in bred squash line and their parents during fruit development. Sci Hortic 136:1–8
Ranwala AP, Miller WB (2000) Purification and characterization of an endoamy-lase from tulip (Tulipa gesneriana) bulbs. Physiol Plantarum 109(4):388–395
Ren ZM, Xia YP, Zhang D, Li Y, Wu Y (2017) Cytological analysis of the bulblet initiation and development in Lycoris species. Sci Hortic 218:72–79
Riou-Khamlichi C, Menges M, Healy JMS, Murray JAH (2000) Sugar control of the plant cell cycle: differential regulation of Arabidopsis D-type cyclin gene expression. Mol Cell Biol 20:4513–4521
Shi S, Qiu Y, Wu L, Fu C (2006) Interspecific relationships of lycoris (amaryllidaceae) inferred from inter-simple sequence repeat data. Sci Hortic 110(3):285–291
Stitt M, Zeeman SC (2012) Starch turnover: pathways, regulation and role in growth. Curr Opin Plant Biol 15(3):282–292
Tsai HL, Lue WL, Lu KJ, Hsieh MH, Wang SM, Chen J (2009) Starch synthesis in Arabidopsis is achieved by spatial cotranscription of core starch metabolism genes. Plant Physiol 151(3):1582–1595
Vriet C, Welham T, Brachmann A, Pike M, Pike J, Perry J, Wang TL (2010) A suite of Lotus japonicus starch mutants reveals both conserved and novel features of starch metabolism. Plant Physiol 154(2):643–655
Xu J, Zha M, Li Y, Ding Y, Chen L, Ding C (2015) The interaction between nitrogen availability and auxin, cytokinin, and strigolactone in the control of shoot branching in rice (oryza sativa, l.). Plant Cell Rep 34(9):1647–1662
Yu SM, Kuo YH, Sheu G, Sheu YJ, Liu L-F (1991) Metabolic derepression of α-amylase gene expression in suspension-cultured cells of rice. J Biol Chem 266:21131–21137
Zuo H, Zhang RQ, Yang ZL, Yang X (2007) Biological characteristics and annual nutrient variation in the corm of Lycoris radiata. Acta Agric Univ Jiangxiensis 29(4):598–602 (in Chinese)
This work was supported by the Shanghai Agriculture Applied Technology Development Program, China (GrantNo. G2016060106) and The Youth Talent Development Plan of Shanghai Municipal Agricultural System, China (Grant No. 20180110).
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Xu, Jx., Li, Qz., Yang, Ly. et al. Changes in Starch Synthesis and Metabolism Within Developing Bulbs of Lycoris radiata During the Vegetative Growth Stage. J Plant Growth Regul 39, 785–794 (2020). https://doi.org/10.1007/s00344-019-10022-1
- Lycoris radiata bulbs
- Starch metabolism
- D-type cyclins