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Application of Streptomyces pactum Act12 Enhances Drought Resistance in Wheat

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Abstract

The use of beneficial microbes to improve drought resistance in crops has great application potential in agricultural production, yet the effects of actinomycetes upon crop resistance to drought are rarely reported. Streptomyces pactum Act12 is a known multi-functional biocontrol agent of soil-borne diseases in several horticultural crops and medicinal plants. Here, we systematically analyzed how Act12 treatment affects drought resistance in drought-sensitive wheat (Triticum aestivum L.) cultivar Xinong 979 by considering both its effects and underlying mechanisms. After seed exposure to a cell-free culture filtrate of Act12, we measured several plant growth variables, osmotic adjustment and antioxidant capacity, cell membrane peroxidation, and drought resistance-related gene expression in wheat seedlings under drought stress conditions simulated by polyethylene glycol 6000. Results showed that, under drought stress, wheat seedling exposure to Act12 cell-free filtrate facilitated plant growth, with significant increases in shoot fresh weight (21.3%), shoot length (10.3%), and root length (13.6%). Act12 treatment also significantly increased total soluble sugar content in wheat leaves while decreasing their malondialdehyde content by 20.5%. Under non-drought conditions, Act12 treatment increased the content of both proline and glutathione in wheat leaves; however, both were lowered in Act12-treated plants compared with non-treated plants at 96 h of drought stress. Further analysis revealed that Act12 treatment increased the content of leaf abscisic acid and upregulated the expression levels of several drought resistance-related genes, such as EXPA2, EXPA6, P5CS, and SnRK2. These results suggest that application of S. pactum Act12 can enhance the osmotic adjustment and antioxidant capacity of plants via induction of abscisic acid accumulation and up-regulation of drought resistance-related gene expression, thereby mitigating drought stress impact in wheat.

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Acknowledgements

This study was funded by the National Natural Science Fund Youth Project (31600407), the Fundamental Research Fund for the Central Universities (Z109021616), and the National Key Technology R&D Program (2012BAD14B11).

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  1. Haiyang Li and Qiao Guo contributed equally to this paper.

Authors and Affiliations

  1. College of Natural Resources and Environment, Northwest A&F University, Yangling, 712100, China

    Haiyang Li, Qiao Guo, Yuexi Jing, Zhe Liu, Zehao Zheng, Yifan Sun, Quanhong Xue & Hangxian Lai

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  1. Haiyang Li
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Li, H., Guo, Q., Jing, Y. et al. Application of Streptomyces pactum Act12 Enhances Drought Resistance in Wheat. J Plant Growth Regul 39, 122–132 (2020). https://doi.org/10.1007/s00344-019-09968-z

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