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Sicwinv1, a Cell Wall Invertase from Sesame, Is Involved in Anther Development

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Abstract

Male sterility is an effective phenotype for sesame hybrid production. Cell wall invertase (CWINV) plays a crucial role in providing carbohydrates for male gametophyte development, and anther-specific repression of CWINV genes in different species causes male sterile plants. In this study, a sesame CWINV gene defined as Sicwinv1 was cloned. Sequence analysis indicated that it contained the conserved β-fructosidase motif and a cysteine catalytic site as well as the glycosylation motif of CWINV proteins. Subcellular localization indicated that Sicwinv1 was localized in the cell wall. Transient overexpression of Sicwinv1 in N. benthamiana plants dramatically increased Sicwinv1 activity in the cell wall of Sicwinv1 overexpression leaves. RT-PCR revealed that Sicwinv1 was particularly expressed in the stamen. Further promoter analysis in Arabidopsis confirmed the anther-specific expression pattern and showed that GUS staining was mainly detected in the anther tapetum. The quantitative GUS assay indicated that the Sicwinv1 promoter drove GUS expression and was correlated with the anther development stage. Consistently, in situ hybridization revealed that Sicwinv1 was expressed in the tetrad stage and microspore stage in sesame and that Sicwinv1 RNA mainly accumulated in the tapetum, and tetrad, microspore, indicating a role of Sicwinv1 in anther development and male fertility. Our results might be an important first step toward unravelling the roles of sesame CWINV and point to a potential for utilizing this gene and its promoter for engineering male sterility for hybrid production in sesame.

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Acknowledgements

This research was supported through funding from the National Natural Science Foundation of China (No. 31701468), China’s National Agricultural Research System (CARS-15) and Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences (CAAS-ASTIP-2013-OCRI).

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Authors and Affiliations

  1. Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture of the People’s Republic of China, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, 430062, China

    Ting Zhou, Guocun Hao, Yuanxiao Yang, Hongyan Liu, Minmin Yang & Yingzhong Zhao

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  1. Ting Zhou
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  2. Guocun Hao
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  3. Yuanxiao Yang
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  4. Hongyan Liu
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  5. Minmin Yang
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  6. Yingzhong Zhao
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Contributions

TZ and YZ designed the research and wrote the manuscript; TZ, GH and YY performed the experiments; HL and MY prepared the materials. All authors read and approved the final manuscript.

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Correspondence to Yingzhong Zhao.

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344_2019_9932_MOESM1_ESM.docx

Fig. S1 Promoter sequence of Sicwinv1. The anther- and/or pollen-specific cis-element was marked with a yellow shadow. (DOCX 15 KB)

344_2019_9932_MOESM2_ESM.tif

Fig. S2 PCR analysis of ProSicwinv1 transgenic lines. Eleven transgenic lines were obtained. WT, wild type; 2, 3, 9, 11, 12, 14, 15, 17, 21, 22, 24 represents 11 transgenic lines. (TIF 961 KB)

344_2019_9932_MOESM3_ESM.tif

Fig. S3 GUS staining of ProSicwinv1-12 transgenic plants. A-B, GUS expression in inflorescence and two-week-old seedlings of ProSicwinv1-12 transgenic line. C, GUS staining in two-week old wild type seedling. (TIF 10851 KB)

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Zhou, T., Hao, G., Yang, Y. et al. Sicwinv1, a Cell Wall Invertase from Sesame, Is Involved in Anther Development. J Plant Growth Regul 38, 1274–1286 (2019). https://doi.org/10.1007/s00344-019-09932-x

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  • DOI: https://doi.org/10.1007/s00344-019-09932-x

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