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Identification and Characterization of ATP/ADP Isopentenyltransferases (ATP/ADP PpIPTs) Genes in Peach

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Abstract

ATP/ADP isopentenyltransferase (IPTs) genes encode key enzymes involved in cytokinin synthesis. In this study, the functions of ATP/ADP PpIPTs in peach were investigated. According to the genome sequence, we have found and verified that there are four members of this gene family in peach, namely, PpIPT1, PpIPT3, PpIPT5, and PpIPT7. Overexpression of each of these genes in Arabidopsis resulted in increased levels of cytokinins in the transgenic plants, confirming their roles in cytokinin synthesis. Numerous altered phenotypes were observed in the transgenic plants, including vigorous growth and enhanced salt resistance. ATP/ADP PpIPTs were expressed in tissues throughout the plant, but the expression patterns differed between the genes. Only PpIPT3 was upregulated within 2 h after the application of nitrate to N-deprived peach seedlings, and the increase was resistant to pre-treatment of a specific nitrate metabolism inhibitor. Results showed that ATP/ADP PpIPT expression levels decreased significantly in pulp within 2 weeks after flowering and remained low. However, pulp cytokinin levels were quite high during this time. Only PpIPT5 in seed increased significantly within 2 weeks after flowering, which was consistent with cytokinin levels during early fruit development, suggesting that PpIPT5 in seed is the key gene for cytokinin biosynthesis during early fruit development. ATP/ADP PpIPT expression also increased significantly during later fruit development in seed.

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Acknowledgements

This work was supported by China Agriculture Research System; CARS-31-3-03; http://119.253.58.231/.

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Authors and Affiliations

  1. Beijing Academy of Forestry and Pomology Sciences, Beijing Academy of Agriculture & Forestry Sciences/Key Laboratory of Urban Agriculture (North China), Ministry of Agriculture, Beijing, 100093, People’s Republic of China

    Min-ji Li & Qin-ping Wei

  2. College of Horticulture Science and Engineering/State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, 271018, Shandong, People’s Republic of China

    Fu-tian Peng, Wen Yu, Jing-jing Luo & Yong-fei Zhao

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  1. Min-ji Li
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  2. Qin-ping Wei
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Correspondence to Fu-tian Peng.

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Li, Mj., Wei, Qp., Peng, Ft. et al. Identification and Characterization of ATP/ADP Isopentenyltransferases (ATP/ADP PpIPTs) Genes in Peach. J Plant Growth Regul 38, 416–430 (2019). https://doi.org/10.1007/s00344-018-9851-6

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