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Epibrassinolide Application Regulates Some Key Physio-biochemical Attributes As Well As Oxidative Defense System in Maize Plants Grown Under Saline Stress

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Abstract

It was aimed to investigate the ameliorative effect of exogenously applied 24-epibrassinolide (EBR) on some key growth parameters and mineral elements in two salt-stressed maize (PR 32T83 and PR 34N24) cultivars. A factorial experiment was designed with two electrical permeability (EC) levels (1.1 and 8.0 dS/m) and two levels (1.5 and 2.0 µM) of EBR supplied as a seed treatment, foliar spray, or both in combination. The foliar application of EBR was done once a week during the experiment. After 42 days of these treatments, the plants were harvested to assess growth, water relations, and oxidative and antioxidative systems. Salt stress markedly reduced plant fresh and dry weights, maximum fluorescence yield of PS-II, chlorophyll contents, leaf water potential, and leaf K and Ca, but it increased membrane permeability, the activities of superoxide dismutase (SOD; EC 1.15.1.1), peroxidase (POD; EC. 1.11.1.7), and catalase (CAT; EC. 1.11.1.6) enzymes, and the contents of proline and glycine betaine, leaf sap osmotic pressure, lipid peroxidation, hydrogen peroxide, and leaf Na and Cl. However, both seed treatment and foliar application of EBR to the maize plants exposed to saline conditions enhanced key growth attributes, water relations, and the activities of various antioxidant enzymes as well as the levels of proline, but they reduced electrolyte leakage, and H2O2 and MDA contents. Saline stress reduced leaf N, Ca2+, K+, and P contents as compared to those in the non-stressed plants. Both seed treatment and foliar application of EBR reduced Na+ and Cl− concentrations, but increased those of N, Ca2+, K+, and P. Foliar application of EBR was more effective in increasing nutrient levels of plants grown at the high saline regime compared to the seed treatment of EBR. The study clearly indicates that both seed treatment and foliar application of EBR at the rate of 2.0 µM can overcome the detrimental effect of salinity stress on maize growth, which was found to be significantly linked to reduced concentrations of Na, Cl, MDA, and H2O2 as well as EL and increased activities of key antioxidant enzymes in the maize plants.

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Acknowledgements

This research was supported by The Scientific and Technical Research Council of Turkey (TUBITAK-TOVAG-114R068).

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Authors and Affiliations

  1. Soil Science and Plant Nutrition Department, Harran University, Sanliurfa, Turkey

    Cengiz Kaya & Salih Aydemir

  2. Department of Botany, GC University Faisalabad, Faisalabad, Pakistan

    Nudrat Aisha Akram

  3. Pakistan Academy of Sciences, Islamabad, Pakistan

    Muhammad Ashraf

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  1. Cengiz Kaya
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  4. Muhammad Ashraf
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CK and SA conducted the experimentation and NAA conducted data analysis. CK and NAA also jointly wrote up the manuscript. MA helped in designing the study and edited critically the whole manuscript.

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Correspondence to Cengiz Kaya.

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Kaya, C., Aydemir, S., Akram, N.A. et al. Epibrassinolide Application Regulates Some Key Physio-biochemical Attributes As Well As Oxidative Defense System in Maize Plants Grown Under Saline Stress. J Plant Growth Regul 37, 1244–1257 (2018). https://doi.org/10.1007/s00344-018-9830-y

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  • DOI: https://doi.org/10.1007/s00344-018-9830-y

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