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Metabolic Conversion of Castasterone and Brassinolide into Their Glucosides in Higher Plants

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Abstract

Castasterone (CS) and brassinolide (BL) were administered to mung bean (Vigna radiata) explants, Arabidopsis thaliana seedlings, and cultured Catharanthus roseus cells, and the glucosylated metabolites were analyzed using LC/MS/MS. In mung bean and C. roseus, CS-2-O-glucoside (CS-2G), -3-O-glucoside (CS-3G), -22-O-glucoside (CS-22G), and -23-O-glucoside (CS-23G) were identified as metabolites of CS, whereas BL-2G, BL-3G, and BL-23G were identified as metabolites of BL. In A. thaliana, CS and BL were converted into their respective 2-O- and 23-O-glucosides. Of the metabolites identified with BL and CS administration, BL-23G was the predominant metabolite in mung bean and A. thaliana, whereas the 3-O-glucoside of BL was abundant in C. roseus. This is the first report of the metabolic conversion of CS into CS-2G, CS-3G, CS-22G, and CS-23G, and of BL into BL-2G and BL-3G. Our results indicate that the glucosylation profiles of BL and CS vary with plant species, and that the glucosylation of CS is rather limited quantitatively, compared with that of BL.

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Authors and Affiliations

  1. RIKEN, Plant Science Center, Tsurumi, Yokohama, 230-0045, Japan

    Kazuo Soeno, Shozo Fujioka, Sayoko Hiranuma, Hideharu Seto & Shigeo Yoshida

  2. RIKEN, Discovery Research Institute, Wako-shi, Saitama, 351-0198, Japan

    Shozo Fujioka, Hideharu Seto & Shigeo Yoshida

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  1. Kazuo Soeno
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  2. Shozo Fujioka
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  3. Sayoko Hiranuma
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  4. Hideharu Seto
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  5. Shigeo Yoshida
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Correspondence to Kazuo Soeno.

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Soeno, K., Fujioka, S., Hiranuma, S. et al. Metabolic Conversion of Castasterone and Brassinolide into Their Glucosides in Higher Plants. J Plant Growth Regul 25, 195–202 (2006). https://doi.org/10.1007/s00344-006-0005-x

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  • DOI: https://doi.org/10.1007/s00344-006-0005-x

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