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Effects of iron and humic acid on competition between Microcystis aeruginosa and Scenedesmus obliquus revealed by HPLC analysis of pigments

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Abstract

Iron is a vital micronutrient for growth of bloom-forming Microcystis aeruginosa and competition with other algae, and its availability is affected by humic acid. The effect of iron and humic acid on growth and competition between M. aeruginosa and Scenedesmus obliquus was assessed. The results showed the growth of M. aeruginosa and S. obliquus in mono-cultures was inhibited by humic acid at low iron concentrations (0.01 mg/L); the maximum inhibition ratios were 67.84% and 38.31%, respectively. The inhibition of humic acid on the two species was significantly alleviated when iron concentrations were 1.00 mg/L, with the maximum inhibition rate reduced to 5.82% for M. aeruginosa and to 23.06% for S. obliquus. S. obliquus was the dominant species in mixed cultures, and the mutual inhibition between M. aeruginosa and S. obliquus at low iron concentration was greater than that at high iron concentration. The inhibition of S. obliquus on M. aeruginosa was reduced at low iron concentrations; it increased at high iron concentrations, as concentrations of humic acid rose.

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Data Availability Statement

Data are available on request to the authors.

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Acknowledgment

We thank Elaine Monaghan, BSc (Econ), from Liwen Bianji, Edanz Editing China (http://www.liwenbianji.cn/ac), for editing the English text of draft of this manuscript.

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Authors and Affiliations

  1. College of Natural Resources and Environment, Northwest A&F University, Yangling, 712100, China

    Miaomiao Zhao, Qiang He, Chongtai Chen, Yaqi Tian, Jing Wei, Haiming Wu & Ming Li

  2. Collaborative Innovation Center of Water Security for Water Source Region of Mid-line of South-to-North Diversion Project of Henan Province, Nanyang Normal University, Nanyang, 473061, China

    Pengfei Duan

  3. Scientific Laboratory of Heyang Agricultural Environment and Farmland Cultivation, Ministry of Agriculture and Rural Affairs, Heyang, 715300, China

    Ming Li

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Supported by the Sichuan Science and Technology Program (No. 2019YFH0127), the Joint Foundation of Shaanxi (No. 2019JLM-59), the Scientific and Technological Innovation Team of Water Ecological Security for Water Source Region of Mid-line of South-to-North Diversion Project of Henan Province (No. 17454)

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Zhao, M., He, Q., Chen, C. et al. Effects of iron and humic acid on competition between Microcystis aeruginosa and Scenedesmus obliquus revealed by HPLC analysis of pigments. J. Ocean. Limnol. 39, 525–535 (2021). https://doi.org/10.1007/s00343-020-0012-y

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