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Effects of vitamin C deficiency or excess on growth performance, anti-oxidative response and fatty acid composition of juvenile abalone Haliotis discus hannai Ino

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Abstract

A 240-day feeding trial was conducted to investigate the effects of dietary vitamin C on growth performance, anti-oxidative response, and fatty acid composition of juvenile abalone Haliotis discus hannai Ino (initial body weight: 0.93±0.00 g). Three semi-purified experimental diets were formulated containing 0.00, 94.52, and 9 649.58 mg/kg of vitamin C supplied as L-ascorbyl-2-monophosphate. The results show that there was no significant difference in weight gain ratio, daily increment in shell length, and survival rate among the three treatments. Adding dietary vitamin C (9 649.58 mg/kg) significantly increased the activities of superoxide dismutase (SOD), glutathine peroxidase (GPX), glitathione-S-transferase (GST), glutathione reductase (GR), alkaline phosphatase (AKP), and lysozyme in viscera (P<0.05). In muscle, activities of phenoloxidase, catalase, SOD, GST, GR, and AKP were increased in the treatment with 9 649.58 mg/kg of dietary vitamin C (P<0.05). The highest concentrations of ascorbic acid in viscera and muscle were found in the group with 9 649.58 mg/kg of dietary vitamin C (P<0.05). The contents of crude protein and crude lipid in the soft body were significantly increased in the 9 649.58 mg/kg group (P<0.05). Dietary vitamin C supplementation significantly decreased the contents of saturated fatty acids (14:0, 16:0, and 18:0), and increased the composition of 18:2n-6 and 22:6n-3 in the soft body of abalone (P<0.05). Therefore, although there were no significant effects on the growth performance, dietary vitamin C supplementation improved the anti-oxidation and immune responses, increased specific unsaturated fatty acid (i.e., 16:1, 18:1n-7, 18:1n-9, 18:2n-6 and 22:6n-3), and decreased specific saturated fatty acid (i.e., 14:0, 16:0 and 18:0) contents in the soft body of abalone.

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Author notes

  1. LI Xinxin and FENG Xiuni contributed equally to this work and should be regarded as co-first authors.

Authors and Affiliations

  1. Key Laboratory of Mariculture (Ministry of Education), Key Laboratory of Aquaculture Nutrition and Feeds (Ministry of Agriculture), Fisheries College, Ocean University of China, Qingdao, 266003, China

    Xinxin Li, Kai Luo, Shuoli Ma, Wenbing Zhang & Kangsen Mai

  2. Jiaozhou Fisheries Technology Promotion Station, Qingdao, 266003, China

    Xiuni Feng

  3. College of Animal Science and Technology, Yunnan Agricultural University, Kunming, 650201, China

    Junming Deng

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  1. Xinxin Li
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Correspondence to Wenbing Zhang.

Additional information

Supported by the National Key R&D Program of China (No. 2018YFD0900400) and the Earmarked Fund for Modern Agro-industry Technology Research System (No. CARS-49)

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Li, X., Feng, X., Luo, K. et al. Effects of vitamin C deficiency or excess on growth performance, anti-oxidative response and fatty acid composition of juvenile abalone Haliotis discus hannai Ino. J. Ocean. Limnol. 38, 1936–1944 (2020). https://doi.org/10.1007/s00343-019-9183-9

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