Journal of Oceanology and Limnology

, Volume 36, Issue 3, pp 1002–1012 | Cite as

Effects of probiotic on microfloral structure of live feed used in larval breeding of turbot Scophthalmus maximus

  • Yan Jiang (姜燕)
  • Zheng Zhang (张正)
  • Yingeng Wang (王印庚)Email author
  • Yayun Jing (景亚运)
  • Meijie Liao (廖梅杰)
  • Xiaojun Rong (荣小军)
  • Bin Li (李彬)
  • Guiping Chen (陈贵平)
  • Hesen Zhang (张和森)


The effects of an exogenous probiotic (Bacillus amyloliquefaciens) on microbial community structure of Branchionus plicatils and Artemia sinica were evaluated in this study during turbot (Scophthalmus maximus) larval breeding. The analysis and comparison of the microfloral composition of live feed with probiotic was conducted using the Illumina HiSeq PE250. The abundance of microbial species and diversity of microflora in live feed with B. amyloliquefaciens were higher than those in the control. The microfloral composition was similar among the three replicate experimental groups of B. plicatils compared with the control after enrichment. Lactococcus, Pseudoalteromonas, and Alteromonas were always dominant. Additionally, some other bacterial species became dominant during the enrichment process. The microbial community during nutrient enrichment of A. sinica was rather similar among the three control replicates. Relative abundance of Cobetia sp., the most dominant species, was 54%–65.2%. Similarity in the microbial community was still high after adding B. amyloliquefaciens. Furthermore, Pseudoalteromonas and Alteromonas replaced Cobetia as the dominant species, and the abundance of Cobetia decreased to 4.3%–25.3%. Mean common ratios at the operational taxonomic unit level were 50%–60% between the two B. plicatils and A. sinica treatments. Therefore, the microbial community structure changed after adding B. amyloliquefaciens during nutrient enrichment of B. plicatils or A. sinica and tended to stabilize. Additionally, the abundance of Vibrio in any kind of live feed was not significantly different from that in the control. These results will help improve the microflora of B. plicatils and A. sinica and can be used to understand the multiple-level transfer role of probiotic species among probiotic products, microflora of live feed, and fish larvae.


Branchionus plicatils Artemia sinica microfloral structure Bacillus amyloliquefaciens Scophthalmus maximus larval breeding 


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Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Yan Jiang (姜燕)
    • 1
  • Zheng Zhang (张正)
    • 1
  • Yingeng Wang (王印庚)
    • 1
    Email author
  • Yayun Jing (景亚运)
    • 1
  • Meijie Liao (廖梅杰)
    • 1
  • Xiaojun Rong (荣小军)
    • 1
  • Bin Li (李彬)
    • 1
  • Guiping Chen (陈贵平)
    • 1
  • Hesen Zhang (张和森)
    • 2
  1. 1.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Laboratory for Marine Fisheries Science and Food Production ProcessesQingdao National Laboratory for Marine Science and TechnologyQingdaoChina
  2. 2.Qingdao General Aquatic Co. Ltd.QingdaoChina

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