Journal of Oceanology and Limnology

, Volume 36, Issue 3, pp 726–737 | Cite as

Chlorophenols in marine organisms from the southern coast of Hangzhou Bay, China, and an assessment of risks posed to human health

  • Dan Zheng (郑丹)
  • Haifeng Jiao (焦海峰)
  • Huiying Zhong (钟惠英)
  • Jishi Qiu (邱纪时)
  • Xiaojun Yan (严小军)Email author
  • Qingyuan Duan (段清源)
  • Liyue Chai (柴丽月)


The composition of chlorophenols in marine organisms from the southern coast of Hangzhou Bay, China, was analyzed and the health risks posed to humans assessed. A total of 19 chlorophenols from 16 types of marine organism were analyzed across nine survey sections in Hangzhou Bay. The chlorophenols were analyzed by gas chromatography-mass spectrometry using a DB-5MS quartz capillary column. The concentrations of monochlorophenol, dichlorophenol, trichlorophenol, tetrachlorophenol, and pentachlorophenol ranged from below the detection limit (ND) to 132 μg/kg, ND–51.0 μg/kg, ND–42.5 μg/ kg, ND–69.0 μg/kg, and ND–9.06 μg/kg, respectively. Additionally, concentration differences between each type of chlorophenol were not significant (P>0.05). However, significant differences were found between monochlorophenol (F=8.13, P<0.01) and total chlorophenol (F=5.19, P<0.01) concentrations. As the noncarcinogenic risk indices were <0.1 (10-5–10-2) for all of the organisms, no high risk was posed by 2-chlorophenol, 2,4-dichlorophenol, 2,4,6-trichlorophenol, 2,4,5-trichlorophenol, 2,3,4,6-tetrachlorophenol, and pentachlorophenol to humans consuming marine organisms from the study area. Furthermore, the carcinogenic risks posed by 2,4,6-trichlorophenol and pentachlorophenol were lower than limits set by the International Commission on Radiological Protection and the US Environmental Protection Agency. However, the noncarcinogenic and carcinogenic risks posed by chlorophenols in marine organisms from four of the survey sections (Sizaopu, Niluoshan, Longshan Town and Xinhong zha) were higher than the other survey sections.


Hangzhou Bay chlorophenols marine organisms health risk 


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The authors wish to thank the many colleagues that assisted with the collection and analysis of marine organism samples.


  1. Besser J M, Wang N, Dwyer F J, Mayer Jr F L, Ingersoll C G. 2005. Assessing contaminant sensitivity of endangered and threatened aquatic species: part II. Chronic toxicity of copper and pentachlorophenol to two endangered species and two surrogate species. Archives of Environmental Contamination and Toxicology, 48 (2): 155–165.Google Scholar
  2. Buikema Jr A L, McGinniss M J, Cairns J. 1979. Phenolics in aquatic ecosystems: a selected review of recent literature. Marine Environmental Research, 2 (2): 87–181.CrossRefGoogle Scholar
  3. Capito B, Potter C, Edwards W. 1997. Comprehensive environmental response compensation and liability act (CERCLA) administrative record management system (ARMS) user’s guide. Revista Geologica de Chile, 35 (2): 279–305.Google Scholar
  4. Chen H L, Yao J, Wang F, Zhou Y, Chen K, Zhuang R S, Choi M M F, Zaray G. 2010. Toxicity of three phenolic compounds and their mixtures on the gram-positive bacteria Bacillus subtilis in the aquatic environment. Science of the Total Environment, 408 (5): 1 043–1 049.CrossRefGoogle Scholar
  5. Cooper G S, Jones S. 2008. Pentachlorophenol and cancer risk: focusing the lens on specific chlorophenols and contaminants. Environmental Health Perspectives, 116 (8): 1 001–1 008.CrossRefGoogle Scholar
  6. Crosby D G. 2009. Environmental chemistry of pentachlorophenol. Pure and Applied Chemistry, 53 (5): 1 051–1 080.CrossRefGoogle Scholar
  7. Czaplicka M. 2004. Sources and transformations of chlorophenols in the natural environment. Science of the Total Environment, 322 (1–3): 21–39.CrossRefGoogle Scholar
  8. de Morais P, Stoichev T, Basto M C, Vasconcelos M T S D. 2012. Extraction and preconcentration techniques for chromatographic determination of chlorophenols in environmental and food samples. Talanta, 89 (2): 1–11.CrossRefGoogle Scholar
  9. Di Giulio R T, Washburn P C, Wenning R J, Winston G W, Jewell C S. 1989. Biochemical responses in aquatic animals: a review of determinants of oxidative stress. Environmental Toxicology and Chemistry, 8 (12): 1 103–1 123.CrossRefGoogle Scholar
  10. do Nascimento N R, Nicola S M C, Rezende M O O, Oliveira T A, Öberg G. 2004. Pollution by hexachlorobenzene and pentachlorophenol in the coastal plain of São Paulo State, Brazil. Geoderma, 121 (3–4): 221–232.CrossRefGoogle Scholar
  11. Dong J, Li X L, Luan T G, Zou S C, Lin L. 2009. Phenol pollution in the sediments of the Pearl River estuary area and its potential risk assessment to the eco-security. Journal of Safety and Environment, 9 (5): 113–116.Google Scholar
  12. Dorsey W C, Tchounwou P B. 2004. Pentachlorophenolinduced cytotoxic, mitogenic, and endocrine-disrupting activities in channel catfish, Ictalurus punctatus. International Journal of Environmental Research and Public Health, 1 (2): 90–99.CrossRefGoogle Scholar
  13. Feng M. 2014. Study on Distribution, Eco-toxicological Effects and Health Risk Assessment of Chlorophenol Contaminants in Dongting Lake. China University of Geosciences, Beijing. p.1-37. (in Chinese)Google Scholar
  14. Häggblom M M, Bossert I D. 2003. Halogenated organic compounds-a global perspective. In: Haggblom M M, Bossert I D eds. Dehalogenation: Microbial Processes and Environmental Applications. Kluwer Academic Publishers, Dordrecht, Netherlands. p.3-29.Google Scholar
  15. Jiang Q T, Lee T K M, Chen K, Wong H L, Zheng J S, Giesy J P, Lo K K W, Yamashita N, Lam P K S. 2005. Human health risk assessment of organochlorines associated with fish consumption in a coastal city in China. Environmental Pollution, 136 (1): 155–165.CrossRefGoogle Scholar
  16. Jin X W, Zha J M, Xu Y P, Giesy J P, Richardson K L, Wang Z J. 2012a. Derivation of predicted no effect concentrations (PNEC) for 2, 4, 6-trichlorophenol based on Chinese resident species. Chemosphere, 86 (1): 17–23.CrossRefGoogle Scholar
  17. Jin X W, Zha J M, Xu Y P, Giesy J P, Wang Z J. 2012b. Toxicity of pentachlorophenol to native aquatic species in the Yangtze River. Environmental Science and Pollution Research, 19 (3): 609–618.CrossRefGoogle Scholar
  18. Johnson W W, Finley M T. 1980. Handbook of acute toxicity of chemicals to fish and aquatic invertebrates. In: US Dept Interior. Fish and Wildlife Service. Resource Publish, Virginia, United States. p.8-87.Google Scholar
  19. Karci A. 2014. Degradation of chlorophenols and alkylphenol ethoxylates, two representative textile chemicals, in water by advanced oxidation processes: the state of the art on transformation products and toxicity. Chemosphere, 99 (3): 1–18.CrossRefGoogle Scholar
  20. Kondo T, Yamamoto H, Tatarazako N, Kawabe K, Koshio M, Hirai N, Morita M. 2005. Bioconcentration factor of relatively low concentrations of chlorophenols in Japanese medaka. Chemosphere, 61 (9): 1 299–1 304.CrossRefGoogle Scholar
  21. Liu H H, Xu Y J, Deng X X, Zhang X X, Zhang H W, Sun Y, Tian X H, Gong X H. 2013. Risk assessment of OCP S and PCB S in organisms in Laizhou Bay offshore areas, Bohai Sea. Oceanologia et Limnologia Sinica, 44 (5): 1 325–1 332. (in Chinese with English abstract)Google Scholar
  22. Liu Q N. 2013. Study on pollution status and health risk assessment of molluscicides and byproduct in Dongting Lake. Hebei Normal University, Shijiazhuang. p.1-54. (in Chinese)Google Scholar
  23. Luo Q, Zha J M, Lei B L, Xu Y P, Wang Z J. 2009. Review of the aquatic ecotoxicology and water quality criteria of three chlorophenols. Acta Scientiae Circumstantiae, 29 (11): 2 241–2 249.Google Scholar
  24. Ma Y B, Han J, Guo Y Y, Lam P K S, Wu R S S, Giesy J P, Zhang X W, Zhou B S. 2012. Disruption of endocrine function in in vitro H295R cell-based and in in vivo assay in zebrafish by 2, 4-dichlorophenol. Aquatic Toxicology, 106-107: 173–181.CrossRefGoogle Scholar
  25. Meng X Z, Zeng E Y, Yu L P, Mai B X, Luo X J, Ran Y. 2007. Persistent halogenated hydrocarbons in consumer fish of China: regional and global implications for human exposure. Environmental Science and Technology, 41 (6): 1 821–1 827.CrossRefGoogle Scholar
  26. Metcalfe J L, Hayton A. 1989. Comparison of leeches and mussels as biomonitors for chlorophenol pollution. Journal of Great Lakes Research, 15 (4): 654–668.CrossRefGoogle Scholar
  27. Murcia M D, Gomenz M, Gomez E. 2007. Degradation of 4-chlorophenol using Pseudomonas putida. Journal of Chemical Engineering, 10: 2–4.Google Scholar
  28. Murray H E, Ray L E, Giam C S. 1981. Analysis of marine sediment, water and biota for selected organic pollutants. Chemosphere, 10 (11-12): 1 327–1 334.CrossRefGoogle Scholar
  29. Olaniran A O, Igbinosa E O. 2011. Chlorophenols and other related derivatives of environmental concern: properties, distribution and microbial degradation processes. Chemosphere, 83 (10): 1 297–1 306.CrossRefGoogle Scholar
  30. Peng Y Q, Chen J H, Lu S Y, Huang J X, Zhang M M, Buekens A, Li X D, Yan J H. 2016. Chlorophenols in municipal solid waste incineration: a review. Chemical Engineering Journal, 292: 398–414.CrossRefGoogle Scholar
  31. Qiu J S, Zhong H Y, Zhu X Y, Sang W G, Duan Q Y, Chai L Y. 2016. Characteristics and ecological risk of selected chlorophenols in seawater of the south Hangzhou Bay. Marine Environmental Science, 35 (2): 231–236. (in Chinese with English abstract)Google Scholar
  32. Rogers I H, Lecings C D, Lockhart W L, Norstrom R J. 1989. Observations on overwintering juvenile Chinook salmon (Oncorhynchus tshawytscha) exposed to bleached Kraft mill effluent in the Upper Fraser River, British Columbia. Chemosphere, 19 (12): 1 853–1 868.CrossRefGoogle Scholar
  33. Strenge D L, Chamberlain P J. 1995. Multimedia environmental pollutant assessment system (MEPAS ®): exposure pathway and human health impact assessment models. Bettelle Memorial Institute, Ohio, U.S. p.2.15-5.19.Google Scholar
  34. Stringer R, Johnston P. 2001. Chlorine and the Environment: An Overview of the Chlorine Industry. Kluwer Academic Publishers, Dordrecht, Netherlands. p.14-38.CrossRefGoogle Scholar
  35. U.S. Environmental Protection Agency. 1992. Guidelines for Exposure Assessment Published on May 29, 1992. Federal Register, 57 (104): 22 888–22 938.Google Scholar
  36. Vlastos D, Antonopoulou M, Konstantinou I. 2016. Evaluation of toxicity and genotoxicity of 2-chlorophenol on bacteria, fish and human cells. Science of the Total Environment, 551-552: 649–655.CrossRefGoogle Scholar
  37. Whelan G, Strenge D L, Droppo Jr J G, Steelman B L, Buck J W. 1987. The remedial action priority system (RAPS): mathematical formulations. Journal of the Australian Traditional-Medicine Society, 19 (1): 13–18.Google Scholar
  38. Yang C F, Lee C M. 2008. Pentachlorophenol contaminated groundwater bioremediation using immobilized Sphingomonas cells inoculation in the bioreactor system. Journal of Hazardous Materials, 152 (1): 159–165.CrossRefGoogle Scholar
  39. Yin D Q, Hu S Q, Jin H J, Yu L W. 2003. Deriving freshwater quality criteria for 2, 4, 6-trichlorophenol for protection of aquatic life in China. Chemosphere, 52 (1): 67–73.CrossRefGoogle Scholar
  40. Zheng W W, Yu H, Wang X, Qu W D. 2012. Systematic review of pentachlorophenol occurrence in the environment and in humans in China: not a negligible health risk due to the re-emergence of schistosomiasis. Environment International, 42: 105–116.CrossRefGoogle Scholar
  41. Zhong H Y, Liu H, Chai L Y, Zheng D, Yang J F, Qiu J S, Qiu J Q. 2016. Determination of 19 chlorophenols and their sodium salts by gas chromatography-mass spectrometry in aquatic products. Journal of Analytical Science, 32 (3): 371–376. (in Chinese with English abstract)CrossRefGoogle Scholar

Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Dan Zheng (郑丹)
    • 1
    • 2
  • Haifeng Jiao (焦海峰)
    • 1
    • 2
  • Huiying Zhong (钟惠英)
    • 2
  • Jishi Qiu (邱纪时)
    • 2
  • Xiaojun Yan (严小军)
    • 1
    Email author
  • Qingyuan Duan (段清源)
    • 2
  • Liyue Chai (柴丽月)
    • 2
  1. 1.School of Marine ScienceNingbo UniversityNingboChina
  2. 2.Ningbo Academy of Oceanology and FisheryNingboChina

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