Chinese Journal of Oceanology and Limnology

, Volume 35, Issue 6, pp 1524–1530 | Cite as

Peritrophin-like protein from Litopenaeus vannamei (LvPT) involved in white spot syndrome virus (WSSV) infection in digestive tract challenged with reverse gavage

  • Shijun Xie (谢世筠)
  • Fuhua Li (李富花)
  • Xiaojun Zhang (张晓军)
  • Jiquan Zhang (张继泉)
  • Jianhai Xiang (相建海)
Aquaculture and Fisheries


The peritrophic membrane plays an important role in the defense system of the arthropod gut. The digestive tract is considered one of the major tissues targeted by white spot syndrome virus (WSSV) in shrimp. In this study, the nucleotide sequence encoding peritrophin-like protein of Litopenaeus vannamei (LvPT) was amplified from a yeast two-hybrid library of L. vannamei. The epitope peptide of LvPT was predicted with the GenScript OptimumAntigen™ design tool. An anti-LvPT polyclonal antibody was produced and shown to specifically bind a band at ~27 kDa, identified as LvPT. The LvPT protein was expressed and its concentration determined. LvPT dsRNA (4 μg per shrimp) was used to inhibit LvPT expression in shrimp, and a WSSV challenge experiment was then performed with reverse gavage. The pleopods, stomachs, and guts were collected from the shrimp at 0, 24, 48, and 72 h post-infection (hpi). Viral load quantification showed that the levels of WSSV were significantly lower in the pleopods, stomachs, and guts of shrimp after LvPT dsRNA interference than in those of the controls at 48 and 72 hpi. Our results imply that LvPT plays an important role during WSSV infection of the digestive tract.


Litopenaeus vannamei digestive tract peritrophin-like protein dsRNA interference binding specificity white spot syndrome virus (WSSV) reverse gavage 


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We sincerely thank the Guangtai Company (Hainan, China) for providing the postlarval shrimp for this study.


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Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Shijun Xie (谢世筠)
    • 1
    • 2
  • Fuhua Li (李富花)
    • 1
    • 3
  • Xiaojun Zhang (张晓军)
    • 1
  • Jiquan Zhang (张继泉)
    • 1
  • Jianhai Xiang (相建海)
    • 1
    • 3
  1. 1.Key Laboratory of Experimental Marine Biology, Institute of OceanologyChinese Academy of SciencesQingdaoChina
  2. 2.University of Chinese Academy of SciencesBeijingChina
  3. 3.Laboratory for Marine Biology and BiotechnologyQingdao National Laboratory for Marine Science and TechnologyQingdaoChina

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