Chinese Journal of Oceanology and Limnology

, Volume 35, Issue 6, pp 1442–1453 | Cite as

Taxonomic revision of Gelidium tsengii and Gelidium honghaiwanense sp. nov. (Gelidiales, Rhodophyta) from China based upon molecular and morphological data analyses

  • Xulei Wang (王旭雷)
  • Bangmei Xia (夏邦美)
  • Antonella Bottalico
  • Guangce Wang (王广策)


The taxonomic relationship of Chinese Gelidium tsengii and Gelidium johnstonii was ambiguous. For almost 20 years they have been regarded as distinct taxa and until 2002 G. johnstonii was considered as a misapplied name of G. tsengii. In this study, herbarium specimens that initially attributed to G. tsengii and fresh G. tsengii specimens were used to address the taxonomic issues. In phylogenetic studies, G. tsengii from Dayawan, China, near the type locality of G. tsengii and G. johnstonii from Sonora, Mexico, the type locality of G. johnstonii, formed a monophyletic group with maximum support in rbcL and COI genes analyses, indicating that they were genetically identical. In morphological studies, G. tsengii was similar to G. johnstonii in branching pattern, inner structures and fructiferous organs. Consequently, we considered that semi-circular outline of G. tsengii could no longer be treated as a discrimating feature. G. johnstonii had priority of publication and according to the International Code of Botanical Nomenclature, G. tsengii was proposed as a synonym of G. johnstonii. Gelidium honghaiwanense sp. nov. was described from Guangdong, China on the basis of morphological and molecular data. For vegetative structures, it was characterized by flattened upright frond, regular two-three times branches pinnate or alternate and clavate ultimate branchlets. For reproductive structures, the tetrasporangial sori were in the apical part of branches and the tetrasporangial branchlets were distichously distributed along second order branches. The present study clarified the relationship between G. tsengii and G. johnstonii from Guangdong and added a new Gelidium species to the Chinese algal flora.


COI Gelidium johnstonii phylogeny rbcL taxonomy 


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Supplementary material

343_2017_5340_MOESM1_ESM.doc (74 kb)
Tab.S1. Specimens of Gelidium spp. examined in the present study.
343_2017_5340_MOESM2_ESM.doc (49 kb)
Tab.S2. New sequences obtained in the present study.


  1. Boo G H, Kim K M, Nelson W A, Riosmena-Rodríguez R, Yoon K J, Boo S M. 2014. Taxonomy and distribution of selected species of the agarophyte genus Gelidium (Gelidiales, Rhodophyta). Journal of Applied Phycology, 26(2): 1243–1251.CrossRefGoogle Scholar
  2. Boo G H, Park J K, Boo S M. 2013. Gelidiophycus (Rhodophyta: Gelidiales): a new genus of marine algae from East Asia. Taxon, 62(6): 1105–1116.CrossRefGoogle Scholar
  3. Chang C F, Xia E Z. 1986. On Gelidium vagum Okam. and G. grubbae Fan. Oceanol. Limnol. Sinica, 17(6): 521–526. (in Chinese with English abstract)Google Scholar
  4. Darriba D, Taboada G L, Doallo R, Posada D. 2012. jModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9(8): 772.CrossRefGoogle Scholar
  5. Fan K C. 1951. The genera Gelidium and Pterocladia of Taiwan. Taiwan Fish. Res. Inst. Lab. Bio. Rep., 2: 1–22.Google Scholar
  6. Fan K C. 1961. Two new species of Gelidium from China. Botanica Marina, 2 (3-4): 247–249.CrossRefGoogle Scholar
  7. Freshwater D W, Fredericq S, Hommersand M H. 1995. A molecular phylogeny of the Gelidiales (Rhodophyta) based on analysis of plastid rbcL nucleotide sequences. Journal of Phycology, 31(4): 616–632.CrossRefGoogle Scholar
  8. Freshwater D W, Rueness J. 1994. Phylogenetic relationships of some European Gelidium (Gelidiales, Rhodophyta) species, based on rbcL nucleotide sequence analysis. Phycologia, 33(3): 187–194.CrossRefGoogle Scholar
  9. Freshwater D W, Tudor K, O’Shaughnessy K, Wysor B. 2010. DNA barcoding in the red algal order Gelidiales: comparison of COI with rbcL and verification of the “barcoding gap”. Cryptogam. Algol., 31(4): 435–449.Google Scholar
  10. Geraldino P J L, Yang E C, Boo S M. 2006. Morphology and molecular phylogeny of Hypnea flexicaulis (Gigartinales, Rhodophyta) from Korea. Algae, 21(4): 417–423.CrossRefGoogle Scholar
  11. Grubb V M. 1932. Marine algae of Korea and China, with notes on the distribution of Chinese marine algae. J. Bot., 70(837): 245–251.Google Scholar
  12. Grusz A L, Freshwater D W. 2014. Studies of Costa Rican Gelidiales (Florideophyceae). II. Two Pacific taxa including Gelidium microglossum, n. sp. Pacific Science, 68(1): 97–110.CrossRefGoogle Scholar
  13. Guindon S, Gascuel O. 2003. A simple, fast, and accurate algorithm to estimate large phylogenies by Maximum Likelihood. Systematic Biology, 52(5): 696–704.CrossRefGoogle Scholar
  14. Guiry M D, Guiry G M. 2015. AlgaeBase. World-wide electronic publication, National University of Ireland, Galway. Scholar
  15. Jeanmougin F, Thompson J D, Gouy M, Higgins D G, Gibson T J. 1998. Multiple sequence alignment with Clustal X. Trends in Biochemical Sciences, 23(10): 403–405.CrossRefGoogle Scholar
  16. Kim K M, Boo S M. 2012. Phylogenetic relationships and distribution of Gelidium crinale and G. pusillum (Gelidiales, Rhodophyta) using cox1 and rbcL sequences. Algae, 27(2): 83–94.CrossRefGoogle Scholar
  17. Kim K M, Gerung G S, Boo S M. 2011a. Two-gene sequences and morphology of Gelidium zollingeri (Kutzing) comb. nov. (Gelidiales, Rhodophyta). Algae, 26(1): 33–40.CrossRefGoogle Scholar
  18. Kim K M, Hwang I K H, Park J K, Boo S M. 2011b. A new agarophyte species, Gelidium eucorneum sp. nov. (Gelidiales, Rhodophyta), based on molecular and morphological data. Journal of Phycology, 47(4): 904–910.CrossRefGoogle Scholar
  19. Kim K M, Hwang I K, Yoon H S, Boo S M. 2012. Four novel Gelidium species (Gelidiales, Rhodophyta) discovered in Korea: G. coreanum, G. jejuensis, G. minimum and G. prostratum. Phycologia, 51(4): 461–474.CrossRefGoogle Scholar
  20. Kützing F T. 1847. Diagnosen einiger neuen ausländischen Algenspecies, welche sich in der Sammlung des Herrn Kammerdirectors Klenze in Laubach befinden. Flora, 30: 773–776.Google Scholar
  21. Lamouroux J V F. 1813. Essai sur les genres de la famille des thalassiophytes non articulées. Annales du Muséum National d’Histoire Naturelle [Paris], 20: 21–47, 115-139, 267-293.Google Scholar
  22. Millar A J K, Freshwater D W. 2005. Morphology and molecular phylogeny of the marine algal order Gelidiales (Rhodophyta) from New South Wales, including Lord Howe and Norfolk Islands. Australian Systematic Botany, 18(3): 215.CrossRefGoogle Scholar
  23. Nelson W A, Farr T J, Broom J E S. 2006. Phylogenetic diversity of New Zealand Gelidiales as revealed by rbcL sequence data. Journal of Applied Phycology, 18(3-5): 653–661.CrossRefGoogle Scholar
  24. Norris J N. 2014. Marine Algae of the Northern Gulf of California II: Rhodophyta. Smithsonian Institution Scholarly Press, Washington, DC. p.1-574.Google Scholar
  25. Okamura K. 1934. On Gelidium and Pterocladia of Japan. Journal of Imperial Fisheries Institute, 29(2): 47–67.Google Scholar
  26. Okamura K. 1935. Taiwan-san Tengusa ni Tsuite Japan. Assoc. Adv. Sci., 10: 441–443. (in Japanese)Google Scholar
  27. Ronquist F, Huelsenbeck J P. 2003. MrBayes 3: bayesian phylogenetic inference under mixed models. Bioinformatics, 19(12): 1572–1574.CrossRefGoogle Scholar
  28. Santelices B. 1988. Taxonomic studies on Chinese Gelidiales (Rhodophyta). In: Abbott I A ed. Taxonomy of Economic Seaweeds: with Reference to Some Pacific and Caribbean Species. California Sea Grant College, University of California, La Jolla, CA. 2: 91–107.Google Scholar
  29. Saunders G W. 2005. Applying DNA barcoding to red macroalgae: a preliminary appraisal holds promise for future applications. Philosophical transactions of the Royal Society B: Biological Sciences, 360(1462): 1879–1888.CrossRefGoogle Scholar
  30. Setchell W A, Gardner N L. 1924. New Marine Algae from the Gulf of California. California Academy of Sciences, San Francisco. p.695-949.Google Scholar
  31. Shimada S. 2000. A systematic study of the order Gelidiales (Rhodophyta) from Japan. Nova Hedwigia, 36: 759–774.Google Scholar
  32. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30(12): 2725–2729.CrossRefGoogle Scholar
  33. Taylor WR. 1943. Marine algae from Haiti collected by H. H. Bartlett in 1941. Michigan Academy of Science, 28: 143–163.Google Scholar
  34. Thomas D T, Freshwater D W. 2001. Studies of Costa Rican Gelidiales (Rhodophyta): four Caribbean taxa including Pterocladiella beachii sp. nov. Phycologia, 40(4): 340–350.CrossRefGoogle Scholar
  35. Tronchin E M, Freshwater D W. 2007. Four Gelidiales (Rhodophyta) new to southern Africa, Aphanta pachyrrhiza gen. et sp. nov., Gelidium profundum sp. nov., Pterocladiella caerulescens and P. psammophila sp. nov. Phycologia, 46(3): 325–348.Google Scholar
  36. Tseng C K, Chang C F, Xia E Z. 1980. Studies on some marine red algae from Hong Kong. In: Morton B S, Tseng C K eds. Proceedings of the First International Marine Biological Workshop: The Marine Flora and Fauna of Hong Kong and Southern China. Hong Kong University Press, Hong Kong. p.57–84.Google Scholar
  37. Tseng C K, Chang T J, Chang C F, Xia E Z, Xia B M, Dong M L, Yang Z D. 1962. Economic Seaweeds of China. Science press, Beijing, China. p.1–198. (in Chinese)Google Scholar
  38. Tseng C K, Cheng P L. 1954. Studies on the marine algae of Tsingtao, I. Acta Bot. Sin., 3(1): 105–120.Google Scholar
  39. Tseng C K, Li L C. 1935. Some marine algae from Tsingtao and Chefoo, Shantung. Bull. Fan. Mem. Inst. Biol. (Bot.), 6(4): 183–235.Google Scholar
  40. Tseng C K. 1938. Notes on some Chinese marine algae. Lingnan Science Journal, 17(4): 591–604.Google Scholar
  41. Tseng C K. 1983. Common Seaweeds of China. Science Press, Beijing, China. p.1–316.Google Scholar
  42. Wang X L, Wang G C, Xia B M. 2016. A new record of genus Gelidium in the Xisha Islands of China, G. pusillum var. pacificum Taylor (Gelidiales, Rhodophyta). Journal of Tropical Oceanography, 35(2): 76–82. (in Chinese with English abstract)Google Scholar
  43. Xia B M, Tseng C K, Wang Y Q. 2002. Synopsis of the Chinese species of Gelidium (Gelidiales, Rhodophyta). Taxonomy of Economic Seaweeds with Reference to Some Pacific Species, 8: 183–205.Google Scholar
  44. Xia B M, Wang Y Q, Xia E Z, Li W X, Ding Z F. 2004. Flora Algarum Marinarum Sinicarum Tomus II Rhodophyta No. III Gelidiales Cryptonemiales Hildenbrandiales. Science Press, Beijing, China. 203p. (in Chinese)Google Scholar
  45. Yamada Y. 1941. Notes on some Japanese algae IX. Scientific papers of the Institute of Algological Research, Hokkaido Imperial University, 2(2): 195–215.Google Scholar
  46. Zhang J F, Xia E Z. 1988. Chinese species of Gelidium Lamouroux and other Gelidials (Rhodophyta), with key, list, and distribution of the common species. In: Abbott I A ed. Taxonomy of Economic Seaweeds: with Reference to some Pacific and Caribbean Species. California Sea Grant College Program, California. p.109–113.Google Scholar

Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Xulei Wang (王旭雷)
    • 1
    • 2
    • 3
  • Bangmei Xia (夏邦美)
    • 4
  • Antonella Bottalico
    • 5
  • Guangce Wang (王广策)
    • 1
    • 2
  1. 1.Key Laboratory of Experimental Marine Biology, Institute of OceanologyChinese Academy of SciencesQingdaoChina
  2. 2.Laboratory for Marine Biology and BiotechnologyQingdao National Laboratory for Marine Science and TechnologyQingdaoChina
  3. 3.University of Chinese Academy of SciencesBeijingChina
  4. 4.Department of Marine Organism Taxonomy and Phylogeny, Institute of OceanologyChinese Academy of SciencesQingdaoChina
  5. 5.Department of BiologyUniversity of Bari A. MoroBariItaly

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