Rb(I)/Se(IV) co-dopant into hydroxyapatite; their structural, morphological, and antibacterial effectiveness for biomedical applications


Co-substituted hydroxyapatite (HAP) with cationic rubidium (Rb+) and anionic selenite (\({\text{SeO}}_{3}^{2 - }\)) was synthesized at different contributions of Rb+ ions. The chemical formula of the composition is [RbxCa(10–0.5x)(PO4)5(SeO3)(OH)2], where 0 ≤ x ≤ 0.8 with step 0.2. The structure and morphology of the as-prepared compositions were investigated upon the concentration of (Rb+) ions. It was mentioned that the roughness average (Ra) starts from 26.2 nm and grows up, reaching 42.6 nm. This noticeable roughness degree due to Rb insertions is reflected in adhesive ability in the biological environment. Rubidium dopant has a significant effect on particle size that inhibits size growth. The internal porosity could be classified as micropores except at x = 0.6 and 0.8, which are considered macro-pores. Rb/Se-HAP compositions' cell viability was investigated through the human osteoblast cell line (HFB4), and the life ratio achieved around 98.1 ± 3.9%. Furthermore, the additional Rb+ ions promote antibacterial activity, whereas the inhibition zone started from 1.6 ± 0.2 and 1.3 ± 0.3 mm and grew up to 5.3 ± 0.9 and 4.9 ± 0.8 mm for x = 0.0 and 0.8, against E. coli and S. aureus, respectively. Thus, the reported stoichiometric compositions are considered to be promising materials for biomedical applications.

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The authors extend their appreciation to the Deanship of Scientific Research at King Khalid University for supporting this work through research groups program under Grant number R.G.P.1/180/41

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Awwad, N.S., Ahmed, M.K., Afifi, M. et al. Rb(I)/Se(IV) co-dopant into hydroxyapatite; their structural, morphological, and antibacterial effectiveness for biomedical applications. Appl. Phys. A 127, 194 (2021). https://doi.org/10.1007/s00339-021-04337-8

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  • Hydroxyapatite
  • antibacterial
  • Cell viability
  • Selenite
  • Rb