Effect of temperature on bacterial community in petroleum hydrocarbon-contaminated and uncontaminated Antarctic soil
It is generally accepted that bacterial diversity in a community confers resistance to environmental perturbation. Communities with high bacterial diversity are less likely to be impacted by environmental changes such as warming. As such, hydrocarbon-contaminated Antarctic soil that are typically characterised by low bacterial diversity and highly selective taxonomic composition are expected to be more sensitive to changes in temperature than uncontaminated Antarctic soil. To test this hypothesis, we evaluated the response of bacterial community structure to warming of hydrocarbon-contaminated and uncontaminated soil collected from Casey Station, Windmill Island, East Antarctica by using microcosms incubated at 5, 10 and 15 °C over a period of 12 weeks. Our results showed that shifts occurred in the bacterial community in relation to the incubation temperatures in both the hydrocarbon-contaminated and uncontaminated soil, with a stronger response observed in the contaminated soil. Taxa referred as comprising hydrocarbon-degrading genera such as Rhodococcus, was the most prevalent genus in the contaminated soil after incubation at 15 °C, accounting for approximately 32–50% of the total detected genera. However, there were no significant differences in the selected functional genes, potentially suggesting high levels of metabolic plasticity in the studied soil bacterial communities. Overall, we showed that hydrocarbon contamination in soil might lead to lower bacterial community stability against environmental perturbation such as temperature variation.
KeywordsAntarctic soil Bacterial community plasticity Functional gene abundance Soil microcosm
The HC and UC Antarctic soils were kindly provided by the Australian Antarctic Division through Australian Antarctic Science Project #4036 (Remediation of Petroleum Contaminants in the Antarctic and subantarctic). This work was funded by UMRG (RP007-2012B) and YPASM fellowship (IMUR121/12). The authors thank University of Malaya and International Medical University for providing research facility and support.
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
- Abdo Z, Schüette UME, Bent SJ, Williams CJ, Forney LJ, Joyce P (2006) Statistical methods for characterizing diversity of microbial communities by analysis of terminal restriction fragment length polymorphisms of 16S rRNA genes. Environ Microbiol 8:929–938. https://doi.org/10.1111/j.1462-2920.2005.00959.x CrossRefPubMedGoogle Scholar
- Adams BJ, Bardgett RD, Ayres E, Wall DH, Aislabie J, Bamforth S, Bargagli R, Cary C, Cavacini P, Connell L, Convey P, Fell JW, Frati F, Hogg ID, Newsham KK, O’Donnell A, Russell N, Seppelt RD, Stevens MI (2006) Diversity and distribution of Victoria Land biota. Soil Biol Biochem 38:3003–3018CrossRefGoogle Scholar
- Adriaenssens EM, Guerrero LD, Makhalanyane TP, Aislabie JM, Cowan DA (2014) Draft genome sequence of the aromatic hydrocarbon-degrading bacterium Sphingobium sp. Strain Ant17, isolated from Antarctic soil. Genome Announc. https://doi.org/10.1128/genomea.00212-14 CrossRefPubMedPubMedCentralGoogle Scholar
- Anderson MJ (2001) A new method for non-parametric multivariate analysis of variance. Austral Ecol 26:32–46Google Scholar
- Anderson MJ, Gorley RN, Clarke KR (2008) PERMANOVA+ for PRIMER: guide to software and statistical methods. PRIMER-E, PlymouthGoogle Scholar
- Bennett JR, Shaw JD, Terauds A, Smol JP, Aerts R, Bergstrom DM, Blais JM, Cheung WWL, Chown SL, Lea MA, Nielsen UN, Pauly D, Reimer KJ, Riddle MJ, Snape I, Stark JS, Tulloch VJ, Possingham HP (2015) Polar lessons learned: long-term management based on shared threats in Arctic and Antarctic environments. Front Ecol Environ 13:316–324. https://doi.org/10.1890/140315 CrossRefGoogle Scholar
- Dias RL, Ruberto L, Calabró A, Balbo AL, Del Panno MT, Mac Cormack WP (2015) Hydrocarbon removal and bacterial community structure in on-site biostimulated biopile systems designed for bioremediation of diesel-contaminated Antarctic soil. Polar Biol 38:677–687. https://doi.org/10.1007/s00300-014-1630-7 CrossRefGoogle Scholar
- Espínola F, Dionisi HM, Borglin S, Brislawn CJ, Jansson JK, Mac Cormack WP, Carroll J, Sjöling S, Lozada M (2018) Metagenomic analysis of subtidal sediments from polar and subpolar coastal environments highlights the relevance of anaerobic hydrocarbon degradation processes. Microb Ecol 75:123–139CrossRefPubMedGoogle Scholar
- Ferguson SH, Powell SM, Snape I, Gibson JAE, Franzmann PD (2008) Effect of temperature on the microbial ecology of a hydrocarbon-contaminated Antarctic soil: implications for high temperature remediation. Cold Reg Sci Technol 53:115–129. https://doi.org/10.1016/j.coldregions.2007.04.006 CrossRefGoogle Scholar
- Isbell F, Craven D, Connolly J, Loreau M, Schmid B, Beierkuhnlein C, Bezemer TM, Bonin C, Bruelheide H, de Luca E, Ebeling A, Griffin JN, Guo Q, Hautier Y, Hector A, Jentsch A, Kreyling J, Lanta V, Manning P, Meyer ST, Mori AS, Naeem S, Niklaus PA, Polley HW, Reich PB, Roscher C, Seabloom EW, Smith MD, Thakur MP, Tilman D, Tracy BF, van der Putten WH, van Ruijven J, Weigelt A, Weisser WW, Wilsey B, Eisenhauer N (2015) Biodiversity increases the resistance of ecosystem productivity to climate extremes. Nature 526:574–577. https://doi.org/10.1038/nature15374 CrossRefPubMedGoogle Scholar
- Muangchinda C, Chavanich S, Viyakarn V, Watanabe K, Imura S, Vangnai AS, Pinyakong O (2015) Abundance and diversity of functional genes involved in the degradation of aromatic hydrocarbons in Antarctic soils and sediments around Syowa Station. Environ Sci Pollut Res 22:4725–4735. https://doi.org/10.1007/s11356-014-3721-y CrossRefGoogle Scholar
- Vazquez S, Monien P, Pepino Minetti R, Jurgens J, Curtosi A, Villalba Primitz J, Frickenhaus S, Abele D, Mac Cormack W, Helmke E (2017) Bacterial communities and chemical parameters in soils and coastal sediments in response to diesel spills at Carlini Station, Antarctica. Sci Total Environ 605–606:26–37CrossRefPubMedGoogle Scholar
- Vázquez S, Nogales B, Ruberto L, Hernandez E, Christie-Oleza J, Lo Balbo A, Bosch R, Lalucat J, Mac Cormack W (2009) Bacterial community dynamics during bioremediation of diesel oil-contaminated Antarctic soil. Microb Ecol 57:598–610. https://doi.org/10.1007/s00248-008-9420-9 CrossRefPubMedGoogle Scholar
- Vázquez S, Nogales B, Ruberto L, Mestre C, Christie-Oleza J, Ferrero M, Bosch R, Mac Cormack WP (2013) Characterization of bacterial consortia from diesel-contaminated Antarctic soils: towards the design of tailored formulas for bioaugmentation. Int Biodeterior Biodegrad 77:22–30. https://doi.org/10.1016/j.ibiod.2012.11.002 CrossRefGoogle Scholar
- Ward NL, Challacombe JF, Janssen PH, Henrissat B, Coutinho PM, Wu M, Xie G, Haft DH, Sait M, Badger J, Barabote RD, Bradley B, Brettin TS, Brinkac LM, Bruce D, Creasy T, Daugherty SC, Davidsen TM, DeBoy RT, Detter JC, Dodson RJ, Durkin AS, Ganapathy A, Gwinn-Giglio M, Han CS, Khouri H, Kiss H, Kothari SP, Madupu R, Nelson KE, Nelson WC, Paulsen I, Penn K, Ren Q, Rosovitz MJ, Selengut JD, Shrivastava S, Sullivan SA, Tapia R, Thompson LS, Watkins KL, Yang Q, Yu C, Zafar N, Zhou L, Kuske CR (2009) Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils. Appl Environ Microbiol 75:2046–2056. https://doi.org/10.1128/aem.02294-08 CrossRefPubMedPubMedCentralGoogle Scholar
- Wertz S, Degrange V, Prosser JI, Poly F, Commeaux C, Guillaumaud N, Le Roux X (2007) Decline of soil microbial diversity does not influence the resistance and resilience of key soil microbial functional groups following a model disturbance. Environ Microbiol 9:2211–2219. https://doi.org/10.1111/j.1462-2920.2007.01335.x CrossRefPubMedGoogle Scholar
- Yang S, Wen X, Shi Y, Liebner S, Jin H, Perfumo A (2016) Hydrocarbon degraders establish at the costs of microbial richness, abundance and keystone taxa after crude oil contamination in permafrost environments. Sci Rep 6:37473. https://doi.org/10.1038/srep37473 CrossRefPubMedPubMedCentralGoogle Scholar