Arabidopsis TCX8 functions as a senescence modulator by regulating LOX2 expression

Abstract

Key message

TCX8 localizes to nucleus and has transcriptional repression activity. TCX8 binds to the promoter region of LOX2 encoding lipoxygenase, causing JA biosynthesis suppression, and thereby delays plant senescence.

Abstract

Conserved CXC domain-containing proteins are found in most eukaryotes. Eight TCX proteins, which are homologs of animal CXC–Hinge–CXC (CHC) proteins, were identified in Arabidopsis, and three of them, TSO1, TCX2/SOL2 and TCX3/SOL1, have been reported to affect cell-cycle control. TCX8, one of the TCX family proteins, was believed to be a TF but its precise function has not been reported. Yeast two-hybrid screening revealed TCP20, a TF that binds to the promoter of LOX2 encoding lipoxygenase, as a strong candidate for interaction with TCX8. We confirmed that TCX8 directly interacts with TCP20 using in vitro pull-down assay and in vivo BiFC and observed that TCX8, as a TF, localizes to nucleus. Using EMSA and by analyzing phenotypes of TCX8-overexpression lines, we demonstrated that TCX8 regulates the expression of LOX2 by binding to either cis-element of LOX2 promoter to which TCP20 or TCP4 binds, affecting JA biosynthesis, and thereby delaying plant senescence. Our study provides new information about the role of TCX8 in modulating plant senescence through regulating LOX2 expression.

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Abbreviations

GUS:

β-Glucuronidase

JA:

Jasmonic acid

LOX:

Lipoxygenase

LUC:

Luciferase

TCX:

Tesmin/TSO1-like CXC domain-containing protein

TF:

Transcription factor

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Acknowledgements

This work was supported by a Grant (PJ01367001 to J.S.S.) from the Next-Generation BioGreen21 Program funded by the Rural Development Administration and by Grants (2019R1F1A1060014 to J.S.S. and 2020R1A6A1A03044344 to J.C.H.) from the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT, Republic of Korea. This work was also partially supported by Korea University.

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MN, SYK and JSS conceived the study; MN and SYK designed experiments; MN, JSS and SYK performed experiments; JCH provided Arabidopsis TF library and helped yeast screening; MN and JSS wrote the manuscript.

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Correspondence to Soo Youn Kim or Jeong Sheop Shin.

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Communicated by Youn-Il Park.

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Noh, M., Shin, J.S., Hong, J.C. et al. Arabidopsis TCX8 functions as a senescence modulator by regulating LOX2 expression. Plant Cell Rep (2021). https://doi.org/10.1007/s00299-021-02663-y

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Keywords

  • Arabidopsis thaliana
  • LOX2
  • Senescence
  • TCP4
  • TCP20
  • TCX8