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Banana MaBZR1/2 associate with MaMPK14 to modulate cell wall modifying genes during fruit ripening

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Abstract

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Banana MaBZR1/2 interact with MaMPK14 to enhance the transcriptional inhibition of cell wall modifying genes including MaEXP2, MaPL2 and MaXET5.

Abstract

Fruit ripening and softening, the major attributes to perishability in fleshy fruits, are modulated by various plant hormones and gene expression. Banana MaBZR1/2, the central transcription factors of brassinosteroid (BR) signaling, mediate fruit ripening through regulation of ethylene biosynthesis, but their possible roles in fruit softening as well as the underlying mechanisms remain to be determined. In this work, we found that MaBZR1/2 directly bound to and repressed the promoters of several cell wall modifying genes such as MaEXP2, MaPL2 and MaXET5, whose transcripts were elevated concomitant with fruit ripening. Moreover, yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays indicated that MaBZR1/2 physically interacted with a mitogen-activated protein kinase MaMPK14, and this interaction strengthened the MaBZR1/2-mediated transcriptional inhibitory abilities. Collectively, our study provides insight into the mechanism of MaBZR1/2 contributing to fruit ripening and softening, which may have potential for banana molecular improvement.

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Abbreviations

1-MCP:

1-Methylcyclopropene

BAK1:

BRI1-associated receptor kinase 1

BES1:

Brassinosteroid Insensitive 1-EMS-Suppressor 1

BiFC:

Bimolecular fluorescence complementation

BIN2:

Brassinosteroid insensitive 2

BR:

Brassinosteroid

BRI1:

Brassinosteroid insensitive 1

BZR1:

Brassinazole resistant 1

EMSA:

Electrophoretic mobility shift assay

EXP:

Expansin

MAPK:

Mitogen-activated protein kinase

MAPKK:

Mitogen-activated protein kinase kinase

MAPKKK:

Mitogen-activated protein kinase kinase kinase

PCR:

Polymerase chain reaction

PL:

Pectate lyase

TF:

Transcription factor

XET:

Xyloglucan endotransglycosylase

Y2H:

Yeast two-hybrid

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Acknowledgements

This work was funded by the National Natural Science Foundation of China (Grant no. 31401922, 31772021), Guangdong Special Support Program (Grant no. 2017TQ04N512), China Agriculture Research System (Grant no. CARS-31-11), and Guangdong Provincial Special Fund For Modern Agriculture Industry Technology Innovation Teams.

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Author notes

  1. Wei Shan and Yu-Fan Guo contributed equally to this work.

Authors and Affiliations

  1. Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Engineering Research Center of Southern Horticultural Products Preservation, Ministry of Education, College of Horticulture, South China Agricultural University, Guangzhou, 510642, People’s Republic of China

    Wei Shan, Yu-Fan Guo, Wei Wei, Jian-Ye Chen, Wang-Jin Lu & Jian-Fei Kuang

  2. Guangdong Food and Drug Vocational College, Longdongbei Road 321, Tianhe District, Guangzhou, 510520, People’s Republic of China

    Xin-Guo Su

  3. Hainan Key Laboratory of Banana Genetic Improvement, Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Haikou, 570102, People’s Republic of China

    De-Bao Yuan

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  1. Wei Shan
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Contributions

JK and XS conceived and designed the research. WS and YG carried out most of the experiments. WW performed some of the experiments. JC, WL, and DY analyzed the data. JK, XS, JC and WL wrote the manuscript. All the authors read and approved the manuscript.

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Correspondence to Xin-Guo Su or Jian-Fei Kuang.

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Shan, W., Guo, YF., Wei, W. et al. Banana MaBZR1/2 associate with MaMPK14 to modulate cell wall modifying genes during fruit ripening. Plant Cell Rep 39, 35–46 (2020). https://doi.org/10.1007/s00299-019-02471-5

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