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A guanine insert in OsBBS1 leads to early leaf senescence and salt stress sensitivity in rice (Oryza sativa L.)

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Abstract

Key message

A rice receptor-like kinase gene OSBBS1/OsRLCK109 was identified; this gene played vital roles in leaf senescence and the salt stress response.

Abstract

Early leaf senescence can cause negative effects on rice yield, but the underlying molecular regulation is not fully understood. bilateral blade senescence 1 (bbs1), an early leaf senescence mutant with a premature senescence phenotype that occurs mainly performing at the leaf margins, was isolated from a rice mutant population generated by ethylmethane sulfonate (EMS) treatment. The mutant showed premature leaf senescence beginning at the tillering stage and exhibited severe symptoms at the late grain-filling stage. bbs1 showed accelerated dark-induced leaf senescence. The OsBBS1 gene was cloned by a map-based cloning strategy, and a guanine (G) insertion was found in the first exon of LOC_Os03g24930. This gene encodes a receptor-like cytoplasmic kinase and was named OsRLCK109 in a previous study. Transgenic LOC_Os03g24930 knockout plants generated by a CRISPR/Cas9 strategy exhibited similar early leaf senescence phenotypes as did the bbs1 mutant, which confirmed that LOC_Os03g24930 was the OsBBS1 gene. OsBBS1/OsRLCK109 was expressed in all detected tissues and was predominantly expressed in the main vein region of mature leaves. The expression of OsBBS1 could be greatly induced by salt stress, and the bbs1 mutant exhibited hypersensitivity to salt stress. In conclusion, this is the first identification of OsRLCKs participating in leaf senescence and playing critical roles in the salt stress response in rice (Oryza sativa L.).

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Abbreviations

bp:

Base pair

CAT:

Catalase

G:

Guanine

POD:

Peroxidase

qRT-PCR:

Reverse transcription-quantitative polymerase chain reaction

RLK:

Receptor-like kinase

RLCK:

Receptor-like cytoplasmic kinase

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

WT:

Wild type

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Acknowledgements

We thank Professor Liu Yaoguang for providing the CRISPR/Cas9 system and Professor Wu Liang for the assistance during vector construction. This work was supported by the Science and Technology Office of Zhejiang Province (2012C12901-2, 2016C32085 and 2016C02050-6).

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Authors and Affiliations

  1. Department of Agronomy, Zhejiang University, Hangzhou, 310058, China

    Dong-Dong Zeng, Cheng-Cong Yang, Ran Qin, Md. Alamin, Er-Kui Yue, Xiao-Li Jin & Chun-Hai Shi

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  1. Dong-Dong Zeng
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  2. Cheng-Cong Yang
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  3. Ran Qin
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  4. Md. Alamin
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  5. Er-Kui Yue
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  7. Chun-Hai Shi
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Contributions

ZDD performed the main experiments and wrote the manuscript. SCH designed the experiment, and edited the final manuscript. YCC and QR measured the physiological indices. AM, YEK and JXL performed the vector constructions and DNA sequencing. All authors have read and approved the final manuscript.

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Correspondence to Chun-Hai Shi.

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The authors declare that they have no conflict of interest.

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Communicated by Zeng-Yu Wang.

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Zeng, DD., Yang, CC., Qin, R. et al. A guanine insert in OsBBS1 leads to early leaf senescence and salt stress sensitivity in rice (Oryza sativa L.). Plant Cell Rep 37, 933–946 (2018). https://doi.org/10.1007/s00299-018-2280-y

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