An endoplasmic reticulum-localized Coffea arabica BURP domain-containing protein affects the response of transgenic Arabidopsis plants to diverse abiotic stresses
The Coffea arabica BURP domain-containing gene plays an important role in the response of transgenic Arabidopsis plants to abiotic stresses via regulating the level of diverse proteins.
Although the functions of plant-specific BURP domain-containing proteins (BDP) have been determined for a few plants, their roles in the growth, development, and stress responses of most plant species, including coffee plant (Coffea arabica), are largely unknown. In this study, the function of a C. arabica BDP, designated CaBDP1, was investigated in transgenic Arabidopsis plants. The expression of CaBDP1 was highly modulated in coffee plants subjected to drought, cold, salt, or ABA. Confocal analysis of CaBDP1-GFP fusion proteins revealed that CaBDP1 is localized in the endoplasmic reticulum. The ectopic expression of CaBDP1 in Arabidopsis resulted in delayed germination of the transgenic plants under abiotic stress and in the presence of ABA. Cotyledon greening and seedling growth of the transgenic plants were inhibited in the presence of ABA due to the upregulation of ABA signaling-related genes like ABI3, ABI4, and ABI5. Proteome analysis revealed that the levels of several proteins are modulated in CaBDP1-expressing transgenic plants. The results of this study underscore the importance of BURP domain proteins in plant responses to diverse abiotic stresses.
KeywordsAbiotic stress Arabidopsis thaliana BURP Coffea arabica Endoplasmic reticulum
We thank Dr. Jung-Hyun Lee for technical assistance to grow coffee plants in a green house. This work was supported by a grant from the Next-Generation BioGreen21 Program (PJ01103601), Rural Development Administration, Republic of Korea.
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Conflict of interest
The authors have no conflict of interest to declare.
- Fuentes D, Meneses M, Nunes-Nesi A, Araújo WL, Tapia R, Gómez I et al (2011) A deficiency in the flavoprotein of Arabidopsis mitochondrial complex II results in elevated photosynthesis and better growth in nitrogen-limiting conditions. Plant Physiol 157:1114–1127CrossRefPubMedPubMedCentralGoogle Scholar
- ICO Annual Review 2012/13. ISSN 1473-3331, 1-36Google Scholar
- Matus JT, Aquea F, Espinoza C, Vega A, Cavallini E, Santo SD et al (2014) Inspection of the grapevine BURP superfamily highlights an expansion of RD22 genes with distinctive expression features in berry development and ABA-mediated stress responses. PLoS ONE 9:e110372CrossRefPubMedPubMedCentralGoogle Scholar
- Park J, Cui Y, Kang BH (2015) AtPGL3 is an Arabidopsis BURP domain protein that is localized to the cell wall and promotes cell enlargement. Frontier Plant Sci 6:412Google Scholar