Plant Cell Reports

, Volume 36, Issue 11, pp 1801–1814 | Cite as

The genetic characteristics in cytology and plant physiology of two wheat (Triticum aestivum) near isogenic lines with different freezing tolerances

  • Wenqiang Wang
  • Qunqun Hao
  • Wenlong Wang
  • Qinxue Li
  • Wei Wang
Original Article


Key message

Freezing tolerance in taft plants relied more upon an ABA-independent- than an ABA-dependent antifreeze signaling pathway.


Two wheat (Triticum aestivum) near isogenic lines (NIL) named tafs (freezing sensitivity) and taft (freezing tolerance) were isolated in the laboratory and their various cytological and physiological characteristics under freezing conditions were studied. Proplastid, cell membrane, and mitochondrial ultrastructure were less damaged by freezing treatment in taft than tafs plants. Chlorophyll, ATP, and thylakoid membrane protein contents were significantly higher, but malondialdehyde content was significantly lower in taft than tafs plants under freezing condition. Antioxidant capacity, as indicated by reactive oxygen species accumulation and antioxidant enzyme activity, and the relative gene expression were significantly greater in taft than tafs plants. Soluble sugars and abscisic acid (ABA) contents were significantly higher in taft plants than in tafs plants under both normal and freezing conditions. The upregulated expression levels of certain freezing tolerance-related genes were greater in taft than tafs plants under freezing treatment. The addition of sodium tungstate, an ABA synthesis inhibitor, led to only partial freezing tolerance inhibition in taft plants and the down-regulated expression of some ABA-dependent genes. Thus, both ABA-dependent and ABA-independent signaling pathways are involved in the freezing tolerance of taft plants. At the same time, freezing tolerance in taft plants relied more upon an ABA-independent- than an ABA-dependent antifreeze signaling pathway.


Near isogenic lines Freezing tolerance Genetic characteristics Antioxidant Abscisic acid 



Near isogenic lines




Nitroblue tetrazolium




Superoxide dismutase




Ascorbate peroxidase


Guaiacol peroxidase








Abscisic acid


Quantitative real-time polymerase chain reaction



This work was supported by the National Natural Science Foundation of China (No. 31370304) and by Funds of Shangdong “Double Tops” Program.

Compliance with ethical standards

Conflict of interest

We declare that we do not have any commercial or associative interest that represents a conflict of interest in connection with the work submitted.

Supplementary material

299_2017_2195_MOESM1_ESM.tif (1.8 mb)
Fig. S1. Thylakoid polypeptides of tafs and taft under control conditions (25°C) and freezing treatment (–20°C) (a), and relative signal densities of corresponding samples of SDS-PAGE in panels (b). M represents molecular mass marker used for SDS-PAGE. Values are mean ± SD based on three replicates. Error bars indicate standard deviations (TIFF 1795 kb)


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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Wenqiang Wang
    • 1
  • Qunqun Hao
    • 2
  • Wenlong Wang
    • 1
  • Qinxue Li
    • 1
  • Wei Wang
    • 1
  1. 1.State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life SciencesShandong Agricultural UniversityTai’anChina
  2. 2.State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of AgronomyShandong Agricultural UniversityTai’anChina

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