Abstract
Key Message
OsiSAP1, an A20/AN1 zinc-finger protein, confers water-deficit stress tolerance at different stages of growth by affecting expression of several endogenous genes in transgenic rice.
Abstract
Transgenic lines have been generated from rice constitutively expressing OsiSAP1, an A20/AN1 zinc-finger containing stress-associated protein gene from rice, driven by maize UBIQUITIN gene promoter and evaluated for water-deficit stress tolerance at different stages of growth. Their seeds show early germination and seedlings grow better under water-deficit stress compared to non-transgenic (NT) rice. Leaves from transgenic seedlings showed lesser membrane damage and lipid peroxidation under water-deficit stress. Relatively lower rate of leaf water loss has been observed in detached intact leaves from transgenic plants during late vegetative stage. Delayed leaf rolling and higher relative water content were also observed in transgenic plants under progressive water-deficit stress during reproductive developmental stage. Although reduction in grain yield is observed under unstressed condition, the relative water-deficit stress-induced yield losses are lower in transgenic rice vis-à-vis NT plants thereby resulting in yield loss protection. Transcriptome analysis suggests that overexpression of OsiSAP1 in transgenic rice results in altered expression of several endogenous genes including those coding for transcription factors, membrane transporters, signaling components and genes involved in metabolism, growth and development. A total of 150 genes were found to be more than twofold up-regulated in transgenic rice of which 43 genes are known to be involved in stress response. Our results suggest that OsiSAP1 is a positive regulator of water-deficit stress tolerance in rice.
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Abbreviations
- ABA:
-
Abscisic acid
- MDA:
-
Malondialdehyde
- MS:
-
Murashige and Skoog
- NT:
-
Non-transgenic
- RGM:
-
Rice growth medium
- SAP:
-
Stress associated protein
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Acknowledgments
This work is supported by grants from the Department of Biotechnology, Government of India. PKD and KSK are grateful to University Grants Commission and Council of Scientific and Industrial Research, respectively, for providing research fellowships during the course of this study.
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Supplementary Fig. 1 Confirmation of transgenic (S2, S3, S10, S105) plants overexpressing OsiSAP1 by PCR of genomic DNA using primers for hygromycin resistance gene (hpt). NT, untransformed control plant; +Ve, plasmid for plant transformation was used as template
Supplementary Fig. 2 Effect of hyperosmotic stress (400 mM mannitol) on seed germination of non-transgenic (NT) and transgenic (S2, S3, S10, S105) rice overexpressing OsiSASP1. Dehusked rice seeds were surface-sterilized and sown on cotton bed saturated with 400 mannitol. Taking radicle and plumule emergence as criteria for seed germination, number of germinated seeds was counted for eight consecutive days. Standard error bar is shown for variation in two independent experiments
Supplementary Fig. 3 Rate of leaf water loss under water-deficit stress in non-transgenic (NT) and transgenic (S2, S3, S10, S105) rice overexpressing OsiSAP1. Data points represent mean ± SE for relative fresh weight of detached intact leaves in two independent experiment (N = 5)
Supplementary tables
Supplementary Table 1 Nucleotide sequences for primers used for confirmation of transgene integration in transgenic rice and evaluation of gene expression analysis by Q-PCR
Supplementary Table 2 Segregation analysis of hygromycin resistance gene (hpt) in T1 generation of transgenic rice overexpressing OsiSAP1
Supplementary Table 3 Microarray expression values and functional categories of genes up-regulated (fold change ≥ 2 and P value < 0.05) in transgenic rice (line S3) overexpressing OsiSAP1 as compared to non-transgenic (NT) plant
Supplementary Table 4 Microarray expression values and functional categories of genes down-regulated (fold change ≥ 2 and P value < 0.05) in transgenic rice (line S3) overexpressing OsiSAP1 as compared to non-transgenic (NT) plant
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Dansana, P.K., Kothari, K.S., Vij, S. et al. OsiSAP1 overexpression improves water-deficit stress tolerance in transgenic rice by affecting expression of endogenous stress-related genes. Plant Cell Rep 33, 1425–1440 (2014). https://doi.org/10.1007/s00299-014-1626-3
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DOI: https://doi.org/10.1007/s00299-014-1626-3