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The low fertility of Chinese white poplar: dynamic changes in anatomical structure, endogenous hormone concentrations, and key gene expression in the reproduction of a naturally occurring hybrid

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We report that low fertility during intraspecific hybridization in Chinese white poplar was caused by prefertilization barriers, reduced ovules, and embryonic abortion. Hormone concentrations and gene expression patterns were also evaluated during the fertilization process.

Abstract

Hybrid vigor holds tremendous potential for yield increases and trait improvement; however, some hybridization combinations within Populus show very low fertility. To explore the causes of this low fertility in intraspecific hybridization of Chinese white poplar, we examined anatomical structure, hormone levels and expression of key genes in two unique crossing combinations of Populus × tomentosa “Pt02” × P. × tomentosa “LM50”, and (P. × tomentosa × P. alba cv. bolleana “Ptb”) × P. × tomentosa “LM50”. The seed set potential in the intraspecific hybridization P. × tomentosa “Pt02” × P. × tomentosa “LM50” was quite low, which was likely caused by prefertilization barriers, reduced ovule numbers, and embryonic abortion in ovaries. During intraspecific hybridization, we found reduced indoleacetic acid (IAA) in pistils, which may cause pollen tube deformations and increased IAA in heart-stage embryos, which may affect embryo development. Gibberellin A3 (GA3) decreased from the zygote dormancy stage to globular-stage embryos, which may be caused by failure of fertilization in specific embryos. The maximum zeatin (Z) concentration was found in heart-stage embryos, but Z concentrations quickly decreased, which may affect endosperm development. Increasing concentrations of abscisic acid (ABA) during zygote dormancy and eight-cell proembryo stages likely induced abscission of the infructescence. High ABA concentrations also regulated embryo maturity. Measurement of genes expression showed that high expression of SRK and/or SLG may result in rejection of pollen by stigmatic papillae through a mechanism, reminiscent of self-incompatibility. Also, low expression of LEC1 and FUS3 may cause embryonic abortion. Identification and eventual bypassing of these barriers may allow future genetic improvement of this key woody crop species.

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Acknowledgments

This work was supported by grants from the following sources: Fundamental Research Funds for Central Universities (No. JC2011-3), and the Project of the National Natural Science Foundation of China (No. 30872042, 31170622).

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Authors and Affiliations

  1. National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, 100083, Beijing, People’s Republic of China

    Kaifeng Ma, Yuepeng Song, Zhen Huang, Liyuan Lin, Zhiyi Zhang & Deqiang Zhang

  2. Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, Beijing, 100083, People’s Republic of China

    Kaifeng Ma, Yuepeng Song, Zhen Huang, Liyuan Lin, Zhiyi Zhang & Deqiang Zhang

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  1. Kaifeng Ma
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  2. Yuepeng Song
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  3. Zhen Huang
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  4. Liyuan Lin
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  5. Zhiyi Zhang
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Correspondence to Deqiang Zhang.

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Communicated by J. S. Shin.

Deceased: Zhiyi Zhang.

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Ma, K., Song, Y., Huang, Z. et al. The low fertility of Chinese white poplar: dynamic changes in anatomical structure, endogenous hormone concentrations, and key gene expression in the reproduction of a naturally occurring hybrid. Plant Cell Rep 32, 401–414 (2013). https://doi.org/10.1007/s00299-012-1373-2

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